163,584 results

1. pAAV-DIO-CAG-hM3Dq-P2A-dTomato

   Plasmid

   #202036

   Purpose

   AAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM3Dq-2A-dTomato

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Insert

   hM3Dq
   

   Use

   AAV

   Tags

   dTomato

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. 2.2

   Plasmid

   #34885

   Depositor

   Irvin Chen

   Article

   Pariente et al Mol Ther. 2007 Nov;15(11):1973-81. Epub

   Insert

   Sindbis virus envelope protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   SGN UTR in E1 domain

   Promoter

   CMV

   Available Since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti X1 Puro DEST (694-6)

   Plasmid

   #17297

   Purpose

   3rd gen lentiviral promoter-less Gateway destination vector, Puro

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi; Destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. GATA3-pCDNA3.1

   Plasmid

   #115271

   Purpose

   Expresses human GATA3 in mammalian cells

   Depositor

   Arne S. Schaefer

   Insert

   GATA3 (GATA3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAJM.661

   Plasmid

   #108532

   Purpose

   TtgRAM + PTtg-YFP Reporter. Nar sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   TtgRAM + PTtg-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   TtgR: R130H A144T A148V

   Promoter

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Dual-CRISPR-Cas9-U6-H1 (pBP48)

   Plasmid

   #218932

   Purpose

   The backbone dual-CRISPR plasmid containing Cas9 that is used for making dual-CRISPR plasmids.

   Depositor

   Baoxu Pang

   Article

   Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   2A-Puro and 3XHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR- flag-Lag9-iCAM-1

   Plasmid

   #205209

   Purpose

   This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG9

   Depositor

   Wendell Lim

   Article

   Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

   Insert

   synCAM iCAM1 and Lag9 anti-GFP nanobody
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. mEos3.2-N1

   Plasmid

   #54525

   Purpose

   Localization: N1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580

   Depositor

   Michael Davidson, Tao Xu

   Article

   Zhang et al Nat Methods. 2012 May 13;9(7):727-9. doi

   Type

   Empty backbone

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. peGFP C3-SPG20

   Plasmid

   #218578

   Purpose

   Expresses GFP-tagged human SPG20 in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

   Insert

   SPG20 (SPART Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-28a mScarlet

    Plasmid

    #178543

    Purpose

    The mScarlet fluorescent protein with an N-terminal 6xHis-tag in pET28a.

    Depositor

    Siegfried Musser

    Article

    Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

    Insert

    mScarlet
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEJS578_DD-dSpyCas9-mCherry-APEX2

    Plasmid

    #108570

    Purpose

    Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Insert

    dSpyCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    APEX2, Destabilization domains, Flag, and mCherry…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. GPR124-Tango

    Plasmid

    #66312

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR124 (ADGRA2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRDA_478

    Plasmid

    #179096

    Purpose

    BE Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    BE3.9 (SpG)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLM433

    Plasmid

    #217293

    Purpose

    For creating CyanoTag vectors by Golden Gate cloning (BspQI version)

    Depositor

    Luke Mackinder

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    mNeonGreen - 3x FLAG

    Expression

    Mutation

    Promoter

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGs-RFP

    Plasmid

    #127347

    Purpose

    Expresses RFP constitutively via the CAGGS promoter in mammalian cells

    Depositor

    Phil Sharp

    Insert

    Red Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS Promoter

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO-Tet-On shYap1-1

    Plasmid

    #193669

    Purpose

    Tet inducible knockdown of Yap1

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    Yap1 (Yap1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe-Kras G12C

    Plasmid

    #58901

    Purpose

    Expresses human Kras with a G12C mutation

    Depositor

    Channing Der

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G12C mutation

    Promoter

    Available Since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUCmini-iCAP-AAV.CAP-B22

    Plasmid

    #175005

    Purpose

    non-standard AAV2 rep-AAV.CAP-B22 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system

    Depositor

    Viviana Gradinaru

    Article

    Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do

    Insert

    Synthetic construct isolate AAV.CAP-B22 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    7 amino acid substitution between VP1 452 and VP1…

    Promoter

    p41

    Available Since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBMN-I-GFP

    Plasmid

    #1736

    Purpose

    This retroviral vector has an IRES-GFP downstream of the cloning sites

    Depositor

    Garry Nolan

    Insert

    IRES-GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCBFD1/HBFD1Bad

    Plasmid

    #53364

    Purpose

    Will convert beta-carotene into astaxanthin in E. coli when expression of the hbfd1 cDNA is induced with arabinose. Use with pAC-BETA or pAC-BETAipi.

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

    Insert

    cbfd1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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