165,688 results

1. pHR_SFFv_4D5-High-CAR_RHL002

   Plasmid

   #164825

   Purpose

   Lentiviral vector for constitutive expression of AntiHER2 4D5-High CAR

   Depositor

   Wendell Lim

   Article

   Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

   Insert

   AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
   

   Use

   Lentiviral

   Promoter

   SFFV

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pActin-miRFP670nano3

   Plasmid

   #184665

   Purpose

   Human beta-actin labelled with near-infrared fluorescent protein miRFP670nano3

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   miRFP670nano3-human beta-actin
   

   Tags

   miRFP670nano3

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENN239_CRISPRcharm_Kv1

   Plasmid

   #220838

   Purpose

   CRISPRcharm Kv1 expression in mammalian cells

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   CRISPRcharm Kv1
   

   Use

   CRISPR

   Tags

   P2A-TagBFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

   Plasmid

   #190033

   Purpose

   vector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promoters

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized hfCas13X
   

   Expression

   Mammalian

   Mutation

   Y672A,Y676A

   Promoter

   CBh, SV40, hU6

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC57-mitoTPNOX

   Plasmid

   #87854

   Purpose

   This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

   Insert

   mitoTPNOX
   

   Tags

   C-terminal FLAG tag with a linker and mitochondri…

   Expression

   Bacterial

   Available Since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB_EF1a_HA-tNGFR_PuroR

   Plasmid

   #212652

   Purpose

   piggyBac vector to express HA-tNGFR with puromycin resistance

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   tNGFR
   

   Use

   Piggybac

   Tags

   HA tag

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3xFLAG-dCas9/pCMV-7.1

   Plasmid

   #47948

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A (catalyticall…

   Promoter

   CMV

   Available Since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJSK664

   Plasmid

   #205130

   Purpose

   Expression of GFP-2xPX (from SnxA) in mammalian cells

   Depositor

   Jason King

   Article

   Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

   Insert

   tandem 2xPX domain of SnxA
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA4/TO-CDC25A

   Plasmid

   #216511

   Purpose

   Expresses CDC25A in human cells under a tetracycline/doxycycline-inducible promoter

   Depositor

   John Diffley

   Article

   Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

   Insert

   CDC25A (CDC25A Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC18T-mini-Tn7T-Gm-dsRedExpress

    Plasmid

    #65032

    Purpose

    GmR mini-Tn7 vector, mobilizable, for RFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    dsRedExpress
    

    Expression

    Bacterial

    Available Since

    Aug. 14, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pHSP02

    Plasmid

    #117259

    Purpose

    Genome editing for Lactobacillus plantarum WCSF1

    Depositor

    Sheng Yang

    Article

    Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

    Insert

    Cas9, promotor P11-guide-RNA, homologous arms of Lp_0537
    

    Use

    Other

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW-tTRE-scFv-SunTag

    Plasmid

    #193138

    Purpose

    Expresses scFv intracellular antibody component of the SunTag system under Dox regulation

    Depositor

    Brad Rosenberg

    Article

    Danziger et al PLoS Pathog. 2022 Apr 14;18(4):e1010464.

    Insert

    scFv-SunTag
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    tight TRE promoter

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSD080A

    Plasmid

    #218138

    Purpose

    Lentiviral vector expressing evolved degron SD40 fused to eGFP with mCherry control.

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    SD40-eGFP-IRES2-mCherry
    

    Use

    Lentiviral

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AiP11390-pAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1390)

    Plasmid

    #163505

    Purpose

    DLX2.0 (3xCore version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain GABAergic interneurons. The enhancer core has 100% seq conservation between mouse and human

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Has Service

    AAV PHP.eB

    Insert

    SYFP2
    

    Use

    AAV

    Promoter

    minBetaGlobin

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHCMV_BoDV1_G

    Plasmid

    #207256

    Purpose

    Mammalian expression of Borna disease virus 1 G protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    BoDV1_G
    

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJL1

    Plasmid

    #69496

    Purpose

    pJL1 expression vector with sfGFP

    Depositor

    Michael Jewett

    Article

    Martin et al Nat Commun. 2018 Mar 23;9(1):1203. doi:

    Insert

    Super folder GFP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. BPK764

    Plasmid

    #65767

    Purpose

    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Bacterial

    Promoter

    T7 (x2)

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti-pegRNA-puro backbone

    Plasmid

    #214085

    Purpose

    Lentiviral backbone plasmid to insert pegRNAs of interest. The cloning site is BsmbI.

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6, EF1a

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcs2-MTS-cox8A-GFP-IRES-mCherry

    Plasmid

    #226103

    Purpose

    Stability reporter construct of the COX8A mitochondrial targeting sequence (MTS) for transient expression in mammalian cells

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Insert

    COX8A (COX8A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    encodes only for mitochondrial targeting sequence…

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE-ERBB3

    Plasmid

    #116735

    Purpose

    Lentiviral expression of ERBB3

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ERBB3 (ERBB3 Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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