163,584 results

1. pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn

   Plasmid

   #214102

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dn

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   PE-Plus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-TLR10-YFP

   Plasmid

   #13643

   Depositor

   Doug Golenbock

   Insert

   Toll-Like Receptor 10 (TLR10 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. AiP13777: pAAV-AiE0682h-minBG-iCre(R297T)-BGHpA (Alias: CN3777)

   Plasmid

   #214847

   Purpose

   Expression of iCre(R297T) in striatal Sst neurons

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

   Insert

   iCre(R297T)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   R297T

   Promoter

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. His6-SpyCatcher-mi3

   Plasmid

   #113043

   Purpose

   Expresses His6-SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

   Depositor

   Mark Howarth

   Article

   Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

   Insert

   His6-SpyCatcher-mi3
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMVTO-KRAS-G12D

   Plasmid

   #179656

   Purpose

   KRAS active mutant (G12D mutation)

   Depositor

   Xiaojing Gao

   Article

   Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

   Insert

   KRAS G12D
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G12D

   Promoter

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLSB-NAT

   Plasmid

   #166698

   Purpose

   Combined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.

   Depositor

   Robin Allshire

   Article

   Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

   Inserts

   tRNA promoter : sgRNA cassette
   adh15 promoter : Cas9 codon-optimised for S. pombe
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   adh15 promoter and tRNA promoter

   Available Since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Yiplac211 proGAL1-TsCC(A)-RGG-GFP-RGG-RGG

   Plasmid

   #176395

   Purpose

   Expresses GFP tagged triple RGG scaffold with N-terminal TsCC(A) coiled coil. Expression induced in media containing galactose.

   Depositor

   Matthew Good

   Article

   Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

   Insert

   GAL1 promoter, TsCC(A)-RGG-GFP-RGG-RGG
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pfa6a PhoCl-TsCC(B)::KANMX

   Plasmid

   #185906

   Purpose

   Plasmid for cloning PhoCl fused to TsCC(B) into yeast.

   Depositor

   Matthew Good

   Article

   Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

   Insert

   PhoCl-TsCC(B)
   

   Use

   Tags

   PhoCl fluorophore with C-terminal TsCC(B)

   Expression

   Yeast

   Mutation

   Promoter

   SP6

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-DIO-CAG-hM4Di-P2A-dTomato

   Plasmid

   #202037

   Purpose

   AAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM4Di-2A-dTomato

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Insert

   hM4Di
   

   Use

   AAV

   Tags

   dTomato

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS316-OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)

    Plasmid

    #198417

    Purpose

    Expresses both OsTIR1F74A and mAID-Nb(VHHGFP4) under the control of ADH1 promoter in budding yeast

    Depositor

    Takumi Kamura

    Article

    Ogawa et al PLoS Genet. 2023 Jun 14;19(6):e1010731.

    Insert

    OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 promoter

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-4T-1-3xFLAG

    Plasmid

    #129570

    Purpose

    Empty backbone for FLAG tag

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR_EF1a-->GssmycLaG16LaG2_P2A_NLSIFP

    Plasmid

    #162228

    Purpose

    Lentiviral vector for the expression of Myc GFP nanbody LaG16LaG2 with IFP NLS in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    LaG16LaG2 and IFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Nuclear cpGFP

    Plasmid

    #215707

    Purpose

    Expresses cpGFP in the nucleus

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    cpGFP
    

    Use

    Lentiviral

    Tags

    FLAG, HA, and NLS

    Expression

    Mammalian

    Mutation

    circularly permuted GFP

    Promoter

    CMV

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV mGAD65(delE1) GFP WPRE SV40pA

    Plasmid

    #177316

    Purpose

    To produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.

    Depositor

    Hirokazu Hirai

    Article

    Hoshino et al Mol Brain. 2021 Feb 15;14(1):33. doi: 10

    Insert

    mGAD65(delE1) promoter, GABAergic interneurons specific promoter
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-Nopt

    Plasmid

    #231704

    Purpose

    Mammalian expression vector encoding a codon-optimized N gene from vesicular stomatitis virus, strain Indiana.

    Depositor

    Ron Geller

    Article

    Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1

    Insert

    VSV N gene (codon-optimized ) (VSIVgp1 vesicular stomatitis virus)
    

    Use

    Vsv expression

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    CMV

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDC191

    Plasmid

    #115792

    Purpose

    Expresses Rat synaptobrevin 2

    Depositor

    Axel Brunger

    Article

    Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

    Insert

    Synaptobrevin2 (Vamp2 Rat)
    

    Use

    Tags

    hexa histidine

    Expression

    Bacterial

    Mutation

    S28C and C103S (please see comments below)

    Promoter

    T7

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDC158

    Plasmid

    #115793

    Purpose

    Expresses Rat SNAP25

    Depositor

    Axel Brunger

    Article

    Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

    Insert

    SNAP25 (Snap25 Rat)
    

    Use

    Tags

    hexa histidine

    Expression

    Bacterial

    Mutation

    c84s, c85s, c90s, c92s

    Promoter

    T7

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEasyCas9

    Plasmid

    #184909

    Purpose

    Expresses the Streptococcus pyogenes Cas9 protein

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

    Plasmid

    #107941

    Purpose

    Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized enAsCas12a (E174R/S542R/K548R)
    

    Use

    Tags

    3x HA and NLS (nucleoplasmin)

    Expression

    Mammalian

    Mutation

    E174R, S542R and K548R

    Promoter

    CAG

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXR003_puroR

    Plasmid

    #219819

    Purpose

    hU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)

    Depositor

    Philipp Maass

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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