165,775 results

1. pMito-MtrxRFP2

   Plasmid

   #172324

   Purpose

   fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)

   Depositor

   Huiwang Ai

   Article

   Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

   Insert

   mitochondrial expression of human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
   

   Tags

   His-tag and Mitochondria localization sequence

   Expression

   Mammalian

   Promoter

   CMV, SP6

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJL43.1 - Pglh-2::MosTase::glh-2utr

   Plasmid

   #19332

   Depositor

   Erik Jorgensen

   Article

   Christian et al

   Insert

   Pglh-2::Mos1 transposase
   

   Expression

   Worm

   Available Since

   Oct. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG ERT2-Cre-ERT2 lox

   Plasmid

   #183447

   Purpose

   CreOFF tamoxifen-inducible Cre

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   ERT2-Cre-ERT2
   

   Expression

   Mammalian

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJRD215

   Plasmid

   #167555

   Purpose

   Backbone plasmid to pRMA154

   Depositor

   Renato Morona

   Article

   Morona et al J Bacteriol. 1994 Feb;176(3):733-47. doi

   Type

   Empty backbone

   Use

   Broad host range mobilisable

   Available Since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-PE2-P2A-hMLH1dn

   Plasmid

   #174827

   Purpose

   Mammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PE2-P2A-hMLH1dn
   

   Tags

   SV40 bpNLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript; deletion of residues 754-…

   Promoter

   CMV

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. APJ-DuET

   Plasmid

   #213185

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   APJ (APLNR Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJL1 sortase

   Plasmid

   #106288

   Purpose

   Expresses sortase in Ecoli off a T7 promoter

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   sortase A pentamutant
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA1-Tat

   Plasmid

   #138478

   Purpose

   Expresses HIV Tat for efficient sgRNA packaging.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   Tat
   

   Expression

   Mammalian

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221_SLC9A3

   Plasmid

   #132234

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC9A3 (SLC9A3 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pTIPI_mIGg2a_Anti-HA [12CA5]

    Plasmid

    #210656

    Purpose

    Mammalian expression of Anti-HA [12CA5] mouse IgG2a heavy chain; to be used with pTIPI_mK_Anti-HA [12CA5] light chain (Plasmid 210657) to make the antibody.

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    Anti-HA [12CA5] mouse IgG2a heavy chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Mouse IgG2a heavy chain

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pTIPI_mK_Anti-HA [12CA5]

    Plasmid

    #210657

    Purpose

    Mammalian expression of Anti-HA [12CA5] mouse kappa light chain; to be used with pTIPI_mIGg2a_Anti-HA [12CA5] heavy chain (Plasmid 210656) to make the antibody.

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    Anti-HA [12CA5] kappa light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Mouse kappa light chain

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. GFP-rab7 WT

    Plasmid

    #12605

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB7 (Homo sapiens) (RAB7A Human)
    

    Tags

    GFP and HA

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCardinal-Lifeact-7

    Plasmid

    #54663

    Purpose

    Localization: Actin, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Type

    Empty backbone

    Tags

    mCardinal-Lifeact

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pHR-PD-1-SNAP

    Plasmid

    #223576

    Purpose

    Lentiviral expression of human PD-1 with SNAP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

    Insert

    PD-1 (PDCD1 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    SNAP

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. tdTomato-H2B-6

    Plasmid

    #58101

    Purpose

    Localization: Nucleus/Histones, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Article

    Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

    Insert

    H2B (H2BC11 Human)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available Since

    Dec. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-Pam-FL

    Plasmid

    #42570

    Depositor

    Vijaya Ramesh

    Article

    Santos et al J Neurosci Res. 2006 Feb 1;83(2):222-32.

    Insert

    human MYCBP2 (MYCBP2 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Pam-FL clone lacks alternatively spliced exons 52…

    Promoter

    CMV

    Available Since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiGuide-puro-NLS-GFP

    Plasmid

    #185473

    Purpose

    Mammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. mCherry-Caveolin-C-10

    Plasmid

    #55008

    Purpose

    Localization: Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Article

    Hanson et al Traffic. 2013 Jun;14(6):663-77. doi: 10.

    Insert

    Caveolin (CAV1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5/FRT-HA-hM3D(Gq)

    Plasmid

    #45547

    Purpose

    Expresses hM3D (Gq) neuronal activator

    Depositor

    Bryan Roth

    Article

    Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

    Insert

    hM3D (Gq)
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    July 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSPObooster

    Plasmid

    #216160

    Purpose

    Plasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strains

    Depositor

    Grant Brown

    Article

    Loll-Krippleber et al Yeast. 2024 Sep 9. doi: 10.1002/yea.3978

    Inserts

    MKT1(30G) (MKT1 Budding Yeast)
    RME1(ins-308A) (RME1 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    MKT1 gene containing an aspartic acid to glycine …

    Promoter

    endogenous promoter

    Available Since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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