159,333 results

1. dCas9-5xGCN4-P2A-BFP

   Plasmid

   #184438

   Purpose

   dCas9 fused with 5 copies of GCN4 epitope

   Depositor

   Rhys Allan

   Article

   Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

   Insert

   dCas9-5xGCN4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKAM-BFP-Puro

   Plasmid

   #101864

   Purpose

   Lentiviral empty backbone, dual BFP and puro markers

   Depositor

   Archibald Perkins

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-dGBP1-TagBFP

   Plasmid

   #80086

   Purpose

   GFP-dependent dGBP1-TagBFP, for expression in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

   Insert

   dGBP1-TagBFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3/er-(n2)oxStayGold(c4)v2.0

   Plasmid

   #186296

   Purpose

   A new version of er-(n2)oxStayGold(c4), which gives a higher expression level. The StayGold gene has codon optimization for mammalian expression. For brighter ER imaging if permitted.

   Depositor

   Atsushi Miyawaki

   Article

   Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

   Insert

   er-(n2)oxStayGold(c4)v2.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AICSDP-36: AAVS1-mEGFP (PGK)

   Plasmid

   #114404

   Purpose

   Homology arms and linker-mEGFP sequence for internal insertion of PGK-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFP

   Depositor

   Allen Institute for Cell Science

   Insert

   AAVS1 Homology Arms with PGK driven mEGFP (AAVS1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   Sept. 4, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. ARHGEF2A-c055

   Plasmid

   #205103

   Purpose

   ARHGEF2 protein expression

   Depositor

   Paul Brennan

   Insert

   ARHGEF2 (ARHGEF2 Human)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. ARHGEF2A-c052

   Plasmid

   #205102

   Purpose

   ARHGEF2 protein expression

   Depositor

   Paul Brennan

   Insert

   ARHGEF2 (ARHGEF2 Human)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. RHOAA-c035

   Plasmid

   #205118

   Purpose

   RHOA protein expression

   Depositor

   Paul Brennan

   Insert

   RHOA (RHOA Human)
   

   Use

   Tags

   6His-TEV and AviTag

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. SpCas9-ABE8e(V106W)

   Plasmid

   #198547

   Purpose

   Expresses human codon-optimized SpCas9-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-ABE8e(V106W)-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-ABE8e(V106W)
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUGW-Cytosol-TurboID

    Plasmid

    #200961

    Purpose

    3rd gen lentiviral plasmid for expressing cytosol localized TurboID with EGFP

    Depositor

    Keriann Backus

    Article

    Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

    Insert

    Cytosol-TurboID
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHIV-H2B-eGFP

    Plasmid

    #91776

    Purpose

    Expresses H2B protein tagged with eGFP

    Depositor

    Maria Pia Cosma

    Article

    Sottile et al Sci Rep. 2016 Nov 9;6:36863. doi: 10.103

    Insert

    Human Histone H2B / eGFP (H2BC11 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. NylCK-TS_pET28a

    Plasmid

    #215423

    Purpose

    Expression construct for NylCK-TS gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylCK-TS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S111G/A137L

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-DIO-ChRmine-mScarlet-Kv2.1-WPRE

    Plasmid

    #130999

    Purpose

    Double floxed soma-targeted ChRmine-mScarlet under the control of Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    mScarlet-Kv2.1

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19/mChe-PM

    Plasmid

    #183168

    Purpose

    Construct for transient expression of plasma membrane-targeted mCherry in plants.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    mCherry-PM
    

    Use

    Tags

    OsRac3, O. sativa rac-like GTP-binding protein 3

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. p101_CRISPRai_PiggyBac

    Plasmid

    #213777

    Purpose

    Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.

    Depositor

    Howard Chang

    Article

    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Inserts

    VPR-dSaCas9
    dSpCas9-KRAB-BFP
    zeocin
    Tet-On transactivator
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EF1a (EF-1-alpha intron a) and Tet_On_TRE

    Available since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDule2-pCNF

    Plasmid

    #85495

    Purpose

    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

    Depositor

    Ryan Mehl

    Article

    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

    Insert

    para-cyanophenylalanine Mj synthetase
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32L L65V F108W Q109M D158G I159P

    Promoter

    lpp (constitutive)

    Available since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. p2T-CMV-evoCjCas9-ABE8e-BlastR

    Plasmid

    #194060

    Purpose

    Mammalian expression of evoCjCas9 ABE8e

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-ABE8e
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAVS1-PDi-CRISPRn

    Plasmid

    #73500

    Purpose

    Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    Cas9
    rtTA
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and TRE

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLR16-Phes*

    Plasmid

    #98783

    Purpose

    Suicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).

    Depositor

    Anat Herskovits

    Article

    Argov et al Appl Environ Microbiol. 2017 Mar 2;83(6)

    Insert

    pheS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A309G

    Promoter

    Available since

    Jan. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRISPR-Cas3

    Plasmid

    #209427

    Purpose

    Plasmid for CASCADE-Cas3 based genome engineering of streptomycetes

    Depositor

    Tilmann Weber

    Article

    Whitford et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

    Insert

    codon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PtipA; crRNA under control of PermE*

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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