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pDASH-DIS-α2

Plasmid

#242520

Purpose

Donor I vector in DASH and alpha2 vector in GoldenBraid/DASH, with SacB counter-selection, with attBTT and attBCC-FRT

Depositor

Jose Alonso, Anna Stepanova

Article

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDASH-DII-α1

Plasmid

#242515

Purpose

Donor II vector in DASH and alpha1 vector in GoldenBraid/DASH, with attPCC and attPTT-FRT

Depositor

Jose Alonso, Anna Stepanova

Article

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS71_10xHis_MBP_TEV_WT-Cas9_2xNLS

Plasmid

#244833

Purpose

Bacterial expression of SpyCas9 (WT) with 2xSV40 nuclear localization sequence for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpyCas9 (WT)

Use

CRISPR

Tags

10xHis-MBP and 2xSV40 NLS

Expression

Bacterial

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS1_10xHis_MBP_TEV_WT-Cas9

Plasmid

#244825

Purpose

Bacterial expression of SpyCas9 (WT) for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpyCas9 (WT)

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDASH-DII-ω2

Plasmid

#242518

Purpose

Donor II vector in DASH and omega2 vector in GoldenBraid/DASH, with attPCC and attPTT-FRT

Depositor

Jose Alonso, Anna Stepanova

Article

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDASH-AIK

Plasmid

#242525

Purpose

Acceptor vector I, accepting Donor I first, with attPTT-FRT

Depositor

Jose Alonso, Anna Stepanova

Article

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14824: pAAV-AiE0441h_3xC2-minBG-iCre-BGHpA (Alias: CN4824)

Plasmid

#214861

Purpose

Expression of iCre in striatal medium spiny neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

iCre

Use

AAV

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Med24-exon minimal CMV pCDNA5

Plasmid

#212060

Purpose

LR vector for integration of Med24-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Med24-exon (Med24 Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Bxb1-LP-TC

Plasmid

#183754

Purpose

AAVS1 targeting construct with the Bxb1 landing pad cassette.

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

loxP-eGFP-attP-Bsd-lox257

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC30A8

Plasmid

#132136

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC30A8 (SLC30A8 Human)

Expression

Mammalian

Available Since

Nov. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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JWW-2 human chimeric monoclonal antibody

Plasmid

#66749

Purpose

Expresses a murine/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 58-64 . JWW-2 works in ELISA/WB/HPV

Depositor

Richard Roden

Article

Wang et al Clin Vaccine Immunol. 2015 May 13. pii:

Inserts

JWW-2 Heavy Chain
JWW-2 Light Chain

Expression

Mammalian

Promoter

mEF1 and rEF1

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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JWW-1 human chimeric antibody

Plasmid

#66748

Purpose

Expresses a rat/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 17-36. JWW-1 works in ELISA/WB/HPV Neutralization assay.

Depositor

Richard Roden

Article

Wang et al Clin Vaccine Immunol. 2015 May 13. pii:

Inserts

JWW-1 Heavy Chain Gamma
JWW-1 Light Chain Kappa

Expression

Mammalian

Mutation

First 3 amino acids of rat light chain constant r…

Promoter

mEF1 and rEF1

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

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dead Sa Cas9

Plasmid

#138162

Purpose

For orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

dSaCas9

Tags

NLS

Expression

Mammalian

Mutation

D10A + N580A

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLdCN

Plasmid

#84290

Purpose

Express gRNA and Cas9 in Leishmania with Neomycin resistance

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

Insert

gRNA and Cas9

Use

CRISPR; Leishmania

Promoter

L. donovani ribosome RNA promoter

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab5A S34N

Plasmid

#28045

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab5A (RAB5A Human)

Tags

EGFP on N-term

Expression

Mammalian

Mutation

S34N

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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Klc1-GFP

Plasmid

#186617

Purpose

Encodes Kinesin Light Chain 1 fused to GFP

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Kinesin Light Chain 1 (KLC1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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psPax2-D64V-NC-mNeon

Plasmid

#196509

Purpose

mNeon fluorescent protein fused to nucleocapsid protein in psPAX2

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

mNeon

Tags

mNeon

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pNCST-AausFP1

Plasmid

#129499

Purpose

Expresses AausFP1 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausFP1

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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SpdCas9 VPR

Plasmid

#188509

Purpose

Expresses FLAG tagged SpdCas9 VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A/H840A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTcI39-switch

Plasmid

#195557

Purpose

Temperature state switch expressing low-temperature mWasabi and high-temperature mRFP1

Depositor

Mikhail Shapiro

Article

Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

Inserts

mRFP1
mWasabi
TcI39

Tags

6x His and AANDENYADAS degradation tag

Expression

Bacterial

Mutation

A67T

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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