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179,813 results

Showing: 6201 - 6220 of 179813 results

  5. pPapi

    Plasmid
    #96921
    Purpose
    expression of S aureus SaCas9 (SauCas9) and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in SpCas9-expressing cell lines for dual Cas9 "Big Papi" screens. aka pXPR207.
    Depositor
    John Doench, David Root
    Publication
    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    June 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    96921-plasmid-map-sequence-id-181715

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  6. TLCV2-RB1

    Plasmid
    #87836
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.
    Depositor
    Adam Karpf
    Publication
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Insert
    sgRB1 (RB1 Human)
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Expression
    Mammalian
    Promoter
    Tight TRE promoter and U6
    Available since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87836-plasmid-map-sequence-id-176310

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  8. pCDNA3-STAT3-Y705F

    Plasmid
    #74434
    Purpose
    Expression of STAT3-Y705F
    Depositor
    Jie Chen
    Publication
    Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Mutations E16K, Y705F
    Promoter
    CMV
    Available since
    May 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74434-plasmid-map-sequence-id-314171

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  12. pHEE2E-TRI

    Plasmid
    #71288
    Purpose
    Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC
    Depositor
    Qi-Jun Chen
    Publication
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA targeting TRY and CPC genes
    sgRNA targeting ETC2 gene
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71288-plasmid-map-sequence-id-166343

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  19. Ii-Str_ssSBP-EGFP

    Plasmid
    #65277
    Purpose
    to synchronize transport of secretory soluble GFP from the ER (RUSH system)
    Depositor
    Franck Perez
    Publication
    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP
    Tags
    IL-2 signal sequence and Streptavidin Binding Pr…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65277-plasmid-map-sequence-id-110485

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  20. GFP-ASH1L

    Plasmid
    #65369
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ASH1L (ASH1L Human)
    Tags
    EmGFP
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    May 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    65369-plasmid-map-sequence-id-437367

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Showing: 6201 - 6220 of 179813 results