159,333 results

1. C1(1-29)-TurboID-V5_pLX304

   Plasmid

   #107175

   Purpose

   expresses V5-tagged TurboID on the mammalian ER membrane, lentiviral vector

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PCDNA3-GFP1-9 T2A mCherry

   Plasmid

   #124430

   Purpose

   Expresses GFP1-9 and T2A mCherry in mammalian cells

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   GFP1-9 T2A mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. MG15-luc (mut p53 binding sites)

   Plasmid

   #16443

   Depositor

   Bert Vogelstein

   Article

   el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

   Insert

   p53-binding site (mutant) (TP53 )
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-HA2-ROC1

   Plasmid

   #19897

   Depositor

   Yue Xiong

   Article

   Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

   Insert

   RING finger protein 1 (ROC1) (RBX1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTP2620

   Plasmid

   #113311

   Purpose

   human Rad50 in baculovirus transfer vector

   Depositor

   Tanya Paull

   Article

   Lee et al J Biol Chem. 2013 May 3;288(18):12840-51

   Insert

   Rad50 (RAD50 Human)
   

   Use

   Tags

   HA, 6xhis

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-SEpHluorin

   Plasmid

   #32001

   Depositor

   Sergio Grinstein

   Article

   Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

   Insert

   mCherry
   

   Use

   Tags

   Superecliptic pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTP813

   Plasmid

   #113308

   Purpose

   human Mre11 in baculovirus transfer vector

   Depositor

   Tanya Paull

   Article

   Bhaskara et al Mol Cell. 2007 Mar 9;25(5):647-61. doi:

   Insert

   Mre11 (MRE11 Human)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. VIPR2-Tango

   Plasmid

   #66521

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   VIPR2 (VIPR2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

9. alpha Synuclein-VC

   Plasmid

   #89471

   Purpose

   expresses human alpha Synuclein fused to the C-terminal part of Venus

   Depositor

   Bradley Hyman, Tiago Outeiro

   Article

   Outeiro et al PLoS One. 2008 Apr 2;3(4):e1867. doi: 10

   Insert

   SNCA (SNCA Human)
   

   Use

   Tags

   C-terminal part of Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB80-hKif5b(1-560)rigor-2xmNeongreen

    Plasmid

    #174649

    Purpose

    Live-cell marker for stable microtubules

    Depositor

    Lukas Kapitein

    Article

    Jansen et al J Cell Biol. 2023 May 1;222(5):e20210610

    Insert

    hKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)
    

    Use

    Tags

    mNeongreen-mNeongreen

    Expression

    Mammalian

    Mutation

    rigor mutation G234A

    Promoter

    Chicken beta-actin

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-smURFP

    Plasmid

    #80349

    Purpose

    Lentiviral transfer vector under the CMV promoter that expresses smURFP.

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Insert

    smURFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFUGW-H1 empty vector

    Plasmid

    #25870

    Purpose

    3rd generation lentiviral vector

    Depositor

    Sally Temple

    Article

    Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    Aug. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmVen-RPA70-IRES-RPA32-IRES-RPA14

    Plasmid

    #211431

    Purpose

    mammalian expression of human RPA70 N-terminally fused to mVenus and RPA32 and RPA14

    Depositor

    Helmut Pospiech

    Insert

    RPA70 - IRES - RPA32 - IRES - RPA14 (RPA1, RPA2, RPA3 Human)
    

    Use

    Tags

    mVenus (on RPA70)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. phage UbiC NLS HA stdMCP stdHalo

    Plasmid

    #104999

    Purpose

    Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labeling

    Depositor

    Jeffrey Chao

    Article

    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

    Insert

    stdMCP-stdHalo
    

    Use

    Lentiviral

    Tags

    HA tag, NLS, Two synonymous copies of the Halo-ta…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    UbiC

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQE60-EFTu-His

    Plasmid

    #124127

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Elongation factor Tu (tufB Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)

    Plasmid

    #214885

    Purpose

    Lentiviral vector encoding TMP-regulatable RfxCas13d-DD fusion

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTol2-nbt-mTagBFP2-P2A-mScarlet

    Plasmid

    #196115

    Purpose

    zebrafish expression of nbt-mTagBFP2-P2A-mScarlet

    Depositor

    Reinhard Köster

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    nbt-mTagBFP2-P2A-mScarlet
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Non tagged Avidin

    Plasmid

    #214835

    Purpose

    This plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al bioRxiv 2024.01.21.576499.

    Insert

    Non tagged Avidin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAcHELIX-sgRNA_entry (CJT94)

    Plasmid

    #181783

    Purpose

    Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-N-Drd1

    Plasmid

    #104358

    Purpose

    Expresses mouse dopamine receptor subtype 1 with EGFP fused to the C-terminus of the receptor. Localizes to primary cilia.

    Depositor

    Kirk Mykytyn

    Article

    Domire et al Cell Mol Life Sci. 2011 Sep;68(17):2951-

    Insert

    Dopamine receptor D1 (Drd1) (Drd1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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