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Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation.
Wang ZP, Xing HL, Dong L, Zhang HY, Han CY, Wang XC, Chen QJ
Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0.
PubMed

Plasmids from Article

ID Plasmid Purpose  
71286pHEE401Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
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71287pHEE401EEgg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
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71288pHEE2E-TRIEgg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC
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91715pKEE401CRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Kan resistance
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91716pXEE401BCRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Bar resistance
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91717pXEE401BGCRISPR/Cas9-mediated genome editing in Arabidopsis, Bar and glyphosate-resistance
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