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pB-rtTA

Plasmid

#126034

Purpose

PiggyBac cargo vector expressing rtTA. Also confers resistance to G418.

Depositor

Mauro Calabrese

Article

Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do

Insert

rtTA3-IRES-Neo cassette cloned from Addgene Plasmid #25735

Use

Piggybac

Expression

Mammalian

Promoter

hUbiC

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pJOG536

Plasmid

#105423

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

Nuclear targeting factor (RanGAP1(WPP)-GFP-BLRP)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLX304-G3BP1-Lantern

Plasmid

#250918

Purpose

Expresses Lantern fused to G3BP1 in mammalian cells for targeting to stress granules

Depositor

Peng Zou

Article

Fang et al Research Square 2025 [Preprint]. https:/

Insert

Lantern

Use

Lentiviral

Tags

V5-G3BP1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGGS-CIBN-EGFP-KRasCT

Plasmid

#178527

Purpose

Expresses CIBN-EGFP-KRasCT in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

CIBN-EGFP-KRasCT

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pH2B-mScarlet3-H (aka mYongHong)

Plasmid

#239404

Purpose

Expresses H2B-mScarlet3-H in mammalian cells

Depositor

Zhifei Fu

Article

Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

Insert

H2B (H2BC21 Human)

Tags

mScarlet3-H (aka mYongHong)

Expression

Mammalian

Mutation

M163H in mScarlet3

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pMJ915

Plasmid

#69090

Purpose

Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

Depositor

Jennifer Doudna

Article

Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

Insert

cas9

Tags

MBP, hexa His, and two SV40 NLS

Expression

Bacterial

Promoter

T7

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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Semaphorin7A-CD4d3+4-bio

Plasmid

#73115

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Semaphorin7A with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Semaphorin 7A (SEMA7A Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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TFORF0782

Plasmid

#141698

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

FOXN4 (FOXN4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57.1_SPTLC1_C133W

Plasmid

#181921

Purpose

3rd generation, Inducible lentiviral plasmid for expression of human mutant SPTLC1 C133W

Depositor

Christian Metallo

Article

Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10

Insert

Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1) mutant C133W (SPTLC1 Human)

Use

Lentiviral; Mammalian expression, , doxycycline i…

Expression

Mammalian

Mutation

cysteine at position 133 of the coding region of …

Promoter

tight TRE promoter

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA4-Myc-HisB-Sirt3

Plasmid

#24918

Depositor

Toren Finkel

Article

Ahn et al Proc Natl Acad Sci U S A. 2008 Sep 23. 1

Insert

Sirt3 (SIRT3 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only
pBabe puro RalB wt

Plasmid

#19720

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog B (RALB Human)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pFastBac HTB DPP9L

Plasmid

#166788

Purpose

Insect cell expression vector for DPP9L (protein purification)

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

HIS-TEV

Expression

Insect

Promoter

polyhedrin

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB DPP9S

Plasmid

#166787

Purpose

Insect cell expression vector for DPP9S (protein purification)

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

HIS-TEV

Expression

Insect

Promoter

polyhedrin

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pUC57-mitoTPNOX

Plasmid

#87854

Purpose

This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

Depositor

Vamsi Mootha

Article

Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

Insert

mitoTPNOX

Tags

C-terminal FLAG tag with a linker and mitochondri…

Expression

Bacterial

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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px459 sgFIP200

Plasmid

#175024

Purpose

CRISPR-Cas9 plasmid targeting exon 3 of human FIP200.

Depositor

David Tumbarello

Article

Assar et al Front Cell Dev Biol. 2020 Aug 4;8:733. d

Insert

RB1CC1 (RB1CC1 Human)

Expression

Mammalian

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSP64T (DM#111)

Plasmid

#15030

Depositor

Douglas Melton

Article

Krieg et al Nucleic Acids Res. 1984 Sep 25. 12(18):7

Insert

beta-globin 5' and 3' UTR

Expression

Bacterial

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

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pCfB8379

Plasmid

#126913

Purpose

Plasmid containing integration cassette at X-4 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI and XdCrtYB

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI and XdCrtYB

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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ICAM4-CD4d3+4-bio

Plasmid

#73102

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding ICAM4 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

ICAM4 (ICAM4 Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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V5-GFP-APEX2-NES_pLX208

Plasmid

#202018

Purpose

expresses GFP-APEX2 in the mammalian cytosol, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

V5-GFP-APEX2-NES

Use

Lentiviral

Tags

GFP and V5

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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ZipGFP-Casp3

Plasmid

#81241

Purpose

A ZipGFP-based Caspase-3 protease reporter T2A mCherry

Depositor

Xiaokun Shu

Article

To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

Insert

ZipGFP with caspase cleavage sequence T2A mCherry

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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