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pBabe-puro-miR-9 sponge
(Plasmid #25040)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 25040 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBabe-puro
  • Backbone manufacturer
    Bob Weinberg, Addgene plasmid 1764
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    miR-9 sponge
  • Insert Size (bp)
    186
  • Tag / Fusion Protein
    • d2EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE 3'
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

8 tandem binding sites to human miR-9. Repeated sequence is TCATACAGCTATATACCAAAGA.

Annealed oligonucleotides for tandem miR-9-binding sites were ligated into the pcDNA5-CMV-d2eGFP vector (Invitrogen) digested with XhoI and ApaI. The gfp mRNA, along with the miR-9 sponge sequence in the 3′ UTR was then subcloned into the pBabe-puro vector digested with BamHI and SalI.

Please note that the total insert size between BamHI and SalI is approximately 1327bp, as it includes d2EGFP, the miR-9 binding sites and a BGH polyA terminator.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe-puro-miR-9 sponge was a gift from Bob Weinberg (Addgene plasmid # 25040 ; http://n2t.net/addgene:25040 ; RRID:Addgene_25040)
  • For your References section:

    miR-9, a MYC/MYCN-activated microRNA, regulates E-cadherin and cancer metastasis. Ma L, Young J, Prabhala H, Pan E, Mestdagh P, Muth D, Teruya-Feldstein J, Reinhardt F, Onder TT, Valastyan S, Westermann F, Speleman F, Vandesompele J, Weinberg RA. Nat Cell Biol. 2010 Mar . 12(3):247-56. 10.1038/ncb2024 PubMed 20173740