pET30-R5-GFP
(Plasmid #17838)

• Depositing Lab: Jay Keasling
• Publication: Shaikh et al Anal Chem. 2008 Feb 1. 80(3):886-90.

Backbone

• Vector backbone: pET30
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5422
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Cloning in E.coli DH10B Protein expression in E.coli BLR(DE3)
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pET30-GFP
• Alt name: pET30-R5-GFP
• Species: Aequorea victoria
• Insert Size (bp): 779
• GenBank ID: AJ851288
• Tags / Fusion Proteins:
  • His (N terminal on backbone)
  • S-tag (N terminal on backbone)
  • R5 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Shaikh, A. S., Tang, Y. J., Mukhopadhyay, A., Keasling, J. D. “Isotopomer distributions in amino acids from a highly expressed protein as a proxy for those from total protein”. Anal Chem. 2008; 80(3):886-890