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(Plasmid #17838)


Item Catalog # Description Quantity Price (USD)
Plasmid 17838 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5422
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Cloning in E.coli DH10B Protein expression in E.coli BLR(DE3)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    Aequorea victoria
  • Insert Size (bp)
  • GenBank ID
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • S-tag (N terminal on backbone)
    • R5 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Shaikh, A. S., Tang, Y. J., Mukhopadhyay, A., Keasling, J. D. “Isotopomer distributions in amino acids from a highly expressed protein as a proxy for those from total protein”. Anal Chem. 2008; 80(3):886-890

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30-R5-GFP was a gift from Jay Keasling (Addgene plasmid # 17838 ; ; RRID:Addgene_17838)
  • For your References section:

    Isotopomer distributions in amino acids from a highly expressed protein as a proxy for those from total protein. Shaikh AS, Tang YJ, Mukhopadhyay A, Keasling JD. Anal Chem. 2008 Feb 1. 80(3):886-90. 10.1021/ac071445+ PubMed 18179240