159,352 results

1. pJ404-DogCatcher-sfGFP

   Plasmid

   #171930

   Purpose

   Express DogCatcher-superfolder GFP protein in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   DogCatcher-sfGFP
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. RpH-ILV-3xALFA

   Plasmid

   #225135

   Purpose

   RpH-ILV probe strongly colocalises with lysosomal markers.

   Depositor

   Massimiliano Stagi

   Article

   Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

   Insert

   TMEM192 (TMEM192 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. mito-EcSTH

   Plasmid

   #211921

   Purpose

   Mitochondrial localization of EcSTH

   Depositor

   Binghui Li

   Article

   Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

   Insert

   mito-EcSTH
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Frb-EGFP-HOTag6

   Plasmid

   #106919

   Purpose

   Express Frb-EGFP-HOTag6 fusion in mammalian cells.

   Depositor

   Xiaokun Shu

   Article

   Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

   Insert

   Frb-EGFP-HOTag6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21a-LeuRS-His

   Plasmid

   #124113

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Leucyl-tRNA ligase (leuS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQC-CherryOFF-GFP

   Plasmid

   #129101

   Purpose

   Reporter vector

   Depositor

   Fangliang Zhang

   Article

   Birnbaum et al Cell Chem Biol. 2018 Aug 16;25(8):1038-1

   Insert

   CherryOFF
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   G-> A

   Promoter

   CMV

   Available since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TEVSH

   Plasmid

   #125194

   Purpose

   His-tagged TEV protease with solubility increasing mutations

   Depositor

   Helena Berglund

   Article

   van den Berg et al J Biotechnol. 2006 Feb 10;121(3):291-8.

   Insert

   TEV_SH
   

   Use

   Tags

   V5 His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21a-CysRS-His

   Plasmid

   #124107

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Cysteinyl-tRNA ligase (cysS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCW57-GFP-P2A-MCS (Neo)

   Plasmid

   #89181

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. nesprin TSmod 25

    Plasmid

    #125555

    Purpose

    Nesprin-2G force sensor with cerulean-F25-venus TSmodule

    Depositor

    Daniel Conway

    Article

    Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

    Insert

    mini-nesprin-2G (Syne2 Mouse)
    

    Use

    Tags

    cerulean, venus

    Expression

    Mammalian

    Mutation

    TSmod changed from mTFP to cerulean, flageliform …

    Promoter

    CMV

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. p663-UBC-miniTurbo-V5-PRKACB_IDG-K

    Plasmid

    #170602

    Purpose

    Gateway destination clone of PRKACB (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-PRKACB (PRKACB Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21a-GlyRS-His

    Plasmid

    #124110

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Glycyl-tRNA ligase (glyS, glyQ Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21a-ProRS-His

    Plasmid

    #124117

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Prolyl-tRNA ligase (proS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. qTAG-C-dTAG-Puro

    Plasmid

    #207727

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pET21a-TrpRS-His

    Plasmid

    #124120

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Tryptophanyl-tRNA ligase (trpS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV.GFAP.iGABASnFR

    Plasmid

    #112172

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    GFAP

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-C1-BBS2

    Plasmid

    #218712

    Purpose

    Expresses N-terminally EGFP-tagged BBS2 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    BBS2 (BBS2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMM553

    Plasmid

    #68543

    Purpose

    NanoLuc expressed constitutively from PBT1311, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Insert

    NanoLuc
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1 mCherry

    Plasmid

    #128073

    Purpose

    puro exchanged to mCherry

    Depositor

    Oskar Laur

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    no

    Promoter

    Available since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-N1_hTRIM32

    Plasmid

    #69541

    Purpose

    Expresses human TRIM32 fused to c-terminal EGFP

    Depositor

    Martin Dorf

    Article

    Fu et al PLoS Pathog. 2015 Jun 9;11(6):e1004960.

    Insert

    tripartite motif containing 32 (TRIM32 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge