165,775 results

1. pyEvolvR (enCas9-PolI5M)

   Plasmid

   #158073

   Purpose

   Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   John Dueber

   Article

   Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

   Insert

   enCas9-PolI5M
   

   Expression

   Bacterial and Yeast

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221-NPC1

   Plasmid

   #132295

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   NPC1 (NPC1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 14, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pP{ELAV-GeneSwitch}

   Plasmid

   #83957

   Purpose

   Expresses conditional RU486-dependent GAL4 protein under control of the elav promotor

   Depositor

   Haig Keshishian

   Article

   Osterwalder et al Proc Natl Acad Sci U S A. 2001 Oct 23;98

   Insert

   fusion protein consisting of the GAL4-DBD, human progesterone receptor-ligand-binding domain, and P65-AD (GAL4, RELA Human, Budding Yeast)
   

   Expression

   Insect

   Promoter

   elav

   Available Since

   Dec. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-HP1beta

   Plasmid

   #17651

   Depositor

   Tom Misteli

   Article

   Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

   Insert

   HP1 beta (CBX1 Human)
   

   Tags

   GFP and His

   Expression

   Mammalian

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETcon(-)

   Plasmid

   #41522

   Depositor

   Andrew Scharenberg

   Type

   Empty backbone

   Tags

   3x(G4S) Linker, G3S Linker, HA, Myc Tag, and Stuf…

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-HP1gamma

   Plasmid

   #17650

   Depositor

   Tom Misteli

   Article

   Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

   Insert

   HP1 gamma (CBX3 Human)
   

   Tags

   GFP and His

   Expression

   Mammalian

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. 476-Desmocollin 2-myc

   Plasmid

   #32233

   Depositor

   Kathleen Green

   Article

   Ishii et al J Invest Dermatol. 2001 Jul;117(1):26-35

   Insert

   Desmocollin 2 (DSC2 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   human beta-actin

   Available Since

   Oct. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pFLAG_G495R MDA5

   Plasmid

   #216799

   Purpose

   Expresses a single point mutant of melanoma differentiation-associated protein 5

   Depositor

   Sun Hur

   Insert

   melanoma differentiation-associated protein 5 (IFIH1 Human)
   

   Expression

   Mammalian

   Mutation

   G495R

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti CMV/TO Hygro empty (w214-1)

   Plasmid

   #17484

   Purpose

   3rd gen lentiviral empty control vector, CMV/TO promoter, Hygro 

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. RHCglo

    Plasmid

    #80169

    Purpose

    Minigene reporter for identification and analysis of cis elements and trans factors affecting pre-mRNA splicing

    Depositor

    Thomas Cooper

    Article

    Singh et al Biotechniques. 2006 Aug;41(2):177-81.

    Insert

    segments of chicken cardiac troponin T (Tnnt2) introns 4 and 5 flanking an artifical exon
    

    Expression

    Mammalian

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCD5-H1MI15-CO-GCN4-TEV-sfGFP-TS

    Plasmid

    #158741

    Purpose

    Expresses influenza A virus trimeric hemagglutinin A/H1/MI/15 fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

    Insert

    Hemagglutinin A/MI/45/15 H1N1
    

    Tags

    GCN4-TEV-sfGFP-TwinStrep

    Expression

    Mammalian

    Mutation

    AA61-469

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. BPK764

    Plasmid

    #65767

    Purpose

    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Bacterial

    Promoter

    T7 (x2)

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-C1-MKLP1

    Plasmid

    #70145

    Purpose

    mammalian expression of MKLP1 with an eGFP fusion

    Depositor

    Masanori Mishima

    Article

    Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

    Insert

    MKLP1 (KIF23 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAcGHLT-B-DDB1

    Plasmid

    #48638

    Purpose

    Expresses Full Lenght DDB1 in Insect Cells (ampR)

    Depositor

    Ning Zheng

    Article

    Li et al Cell. 2006 Jan 13;124(1):105-17.

    Insert

    DDB1 (DDB1 Human)
    

    Expression

    Insect

    Available Since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pCMV-PEmaxRNaseHtrunc

    Plasmid

    #207858

    Purpose

    Mammalian expression of PEmaxDRNaseH prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PEmaxDRNaseH
    

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    M-MLVRTDRNAseHrt

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRL443

    Plasmid

    #70261

    Purpose

    Shuttle vector suitable for mobilizing plasmids to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strains.

    Depositor

    Peter Wolk

    Article

    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

    Type

    Empty backbone

    Available Since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBABE-neo largeTcDNA

    Plasmid

    #1780

    Purpose

    Retroviral expression of Large T Antigen. Used to create immortalized cell lines.

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    large T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHSP02

    Plasmid

    #117259

    Purpose

    Genome editing for Lactobacillus plantarum WCSF1

    Depositor

    Sheng Yang

    Article

    Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

    Insert

    Cas9, promotor P11-guide-RNA, homologous arms of Lp_0537
    

    Use

    Other

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNA 3C myc scfvFLAG

    Plasmid

    #234990

    Purpose

    Expression of transmembrane region of Neuraminidase protein fused to an anti FLAG scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG protein

    Depositor

    Monica Gonzalez-Magaldi, Daniel Leahy

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    NA-3C-myc-scfvFLAG
    

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 EYFP H148Q/I152L

    Plasmid

    #25872

    Depositor

    Peter Haggie

    Article

    Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

    Insert

    EYFP H148Q/I152L
    

    Expression

    Mammalian

    Mutation

    EYFP from pEYFP (Clontech) with H148Q and I152L m…

    Available Since

    Aug. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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