161,709 results

1. pLV6-mPER2-luc

   Plasmid

   #212035

   Purpose

   Expression of murine Per2-luciferase in mammalian cells

   Depositor

   David Hazlerigg

   Article

   Appenroth et al J Biol Rhythms. 2024 Dec;39(6):554-567.

   Insert

   mPer2-luciferase (Per2 Mouse)
   

   Use

   Lentiviral and Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry-CaaX(Hras)

   Plasmid

   #108886

   Purpose

   Expression of membrane localized mCherry

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   mCherry-CaaX(Hras)
   

   Use

   Can be used for mammalian expression or in vitro …

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-PIK3CD

   Plasmid

   #174190

   Purpose

   Expresses PIK3CD in mammalian cells

   Depositor

   Cheng-han Yu

   Article

   Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

   Insert

   PIK3CD (PIK3CD Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223-BRD4

   Plasmid

   #23455

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

5. anxA2-GFP

   Plasmid

   #107196

   Purpose

   Expresses human annexin A2-GFP fusion protein for live cell imaging

   Depositor

   Ursula Rescher, Volker Gerke

   Article

   Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

   Insert

   annexin A2 (ANXA2 Human)
   

   Use

   Tags

   Green Fluorescent Protein

   Expression

   Mammalian

   Mutation

   Ala residue 65 in human sequence replaced by Glu …

   Promoter

   CMV

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRC_RR_VP1_r1c3

   Plasmid

   #65724

   Purpose

   Rescued infectious variant from pAAV2_RaPID library; mCherry insertion in place of the deleted amino acid sequence 453-GTTTQSR

   Depositor

   Junghae Suh

   Article

   Judd et al Mol Ther Nucleic Acids. 2012 Nov 13;1:e5

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF6-CSPG4-myc-his

   Plasmid

   #69037

   Purpose

   Expression of CSPG4 in mammalian cells

   Depositor

   Wensheng Wei

   Article

   Yuan et al Cell Res. 2015 Feb;25(2):157-68. doi: 10

   Insert

   CSPG4 (CSPG4 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1 alpha

   Available since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. GPR26-Tango

   Plasmid

   #66348

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR26 (GPR26 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pOTTC407 - pAAV EF1a eGFP

   Plasmid

   #60058

   Purpose

   An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

   Insert

   enhanced Green Fluorescent Protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. CROPseq-multi-Puro (pRW1441)

    Plasmid

    #216217

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi construct with Puromycin resistance

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBFv-U6.2

    Plasmid

    #138400

    Purpose

    gRNA expression vector for Drosophila

    Depositor

    Shu Kondo

    Article

    Kondo et al Genetics. 2013 Nov;195(3):715-21. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6-2

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. SNAPtag-PTS1

    Plasmid

    #182883

    Purpose

    Express SNAPtag-PTS1 in mammalian cells. SNAPtag targets to peroxisomes

    Depositor

    Pekka Katajisto

    Article

    Bui et al Nat Commun. 2025 Apr 26;16(1):3932. doi:

    Insert

    SNAPtag-PTS1
    

    Use

    Lentiviral

    Tags

    SNAPtag

    Expression

    Mammalian

    Mutation

    no

    Promoter

    CMV

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pATetO 6XHis-Taq DNA Pol

    Plasmid

    #178782

    Purpose

    Inducible expression of Taq DNA polymerase in E. coli

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

    Insert

    Taq DNA Polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBA559

    Plasmid

    #186235

    Purpose

    pTet-LbuCas13a crRNA entry vector

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFB2E

    Plasmid

    #195288

    Purpose

    Bicistronic HSV-TK-EGFP fusion and Puromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    HSV-TK EGFP 2A Puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-BRD4ΔN-mCherry-sspB

    Plasmid

    #121968

    Purpose

    Expresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID.

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-sspB

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1-461

    Promoter

    SFFV

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-OGT_opt

    Plasmid

    #190858

    Purpose

    For mammalian expression of wild-type full-length human ncOGT. The ncOGT is not epitope-tagged, but is codon-optimized for human cells.

    Depositor

    Gerald Hart

    Article

    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codon optimized for expression in human cells.

    Promoter

    CMV

    Available since

    Jan. 9, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pJMK074: CMV QuasAr2-TS-GCaMP6f

    Plasmid

    #72303

    Purpose

    Combines a gCaMP-based calcium indicator and an Arch-based voltage indicator

    Depositor

    Adam Cohen

    Article

    Dempsey et al J Pharmacol Toxicol Methods. 2016 May 13

    Insert

    CaViar
    

    Use

    Lentiviral

    Tags

    TS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tol2-CAG::Palmbow

    Plasmid

    #158993

    Purpose

    Multicolor membrane-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing palmitoylated mCherry, mEYFP or mCerulean upon Cre recombination)

    Depositor

    Jean Livet

    Article

    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

    Insert

    Palmbow
    

    Use

    Tags

    The default expressed EBFP2 is fused to H2B; oth…

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 phiC31

    Plasmid

    #68310

    Purpose

    In vitro transcription vector to generate phiC31 mRNA (i.e. for zebrafish injection).

    Depositor

    Konrad Basler

    Article

    Bischof et al Proc Natl Acad Sci U S A. 2007 Feb 27;10

    Insert

    phiC31
    

    Use

    Rna transcription

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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