161,805 results

1. pCDNA-FLAG-METTL3

   Plasmid

   #160250

   Purpose

   Express FLAG-METTL3 in human cells

   Depositor

   Alessandro Fatica

   Article

   Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

   Insert

   methyltransferase like 3 (METTL3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCF806-shRen.713

   Plasmid

   #186712

   Purpose

   Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Non-targeting control shRNA (shRen.713).

   Depositor

   Christof Fellmann

   Article

   Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

   Insert

   shRen.713
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR_PGK_SNIPR_Hinge Notch SNIPR (Notch2 JMD)

   Plasmid

   #188375

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. OptoEphB2KD-mCherry

   Plasmid

   #92044

   Purpose

   Expresses kinase-dead mutant of OptoEphB2 labeled with mCherry

   Depositor

   Ji Yu

   Insert

   EPHB2 (EPHB2, CRY2 Mustard Weed, Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiCRISPR - EGFP sgRNA 1

   Plasmid

   #51760

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

   Inserts

   Cas9
   Puromycin resistance
   EGFP sgRNA 1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. 426Gal-FUS

   Plasmid

   #29591

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 1, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pCMV-2NLS-Cas9-6XMS2

   Plasmid

   #166033

   Purpose

   Expression of 6X MS2 stem loops fused SpCas9 mRNA for packaging of SpCas9 mRNA within lentiviral particles.

   Depositor

   Yujia Cai

   Article

   Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP

   Plasmid

   #195505

   Purpose

   T-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   pT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)
   

   Use

   CRISPR; Donor vector

   Tags

   mRuby-3xFLAG-NLS-3xSV40-poly(A)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. FKBP:FRB-FBAR

   Plasmid

   #223620

   Purpose

   Transient expression of human FBP17 with mGFP, FRB, FKBP and TagBFP tags in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   FBP17 (FNBP1 Human)
   

   Use

   Tags

   FKBP, TagBFP and mGFP, FRB

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSNAP-macroH2A1.2 Zeo

    Plasmid

    #132428

    Purpose

    Expressing SNAP-tagged histone macroH2A1.2 in mammalian cells

    Depositor

    John M. Greally

    Article

    Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

    Insert

    macroH2A1.2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-CAG-flex-GFP-4x6T

    Plasmid

    #196418

    Purpose

    Cre-dependent AAV expression of GFP preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    GFP
    

    Use

    AAV and Cre/Lox; Astrocyte-selective

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. R26P-M2rtTA

    Plasmid

    #47381

    Purpose

    Rosa 26 targeting vector

    Depositor

    Rudolf Jaenisch

    Article

    Beard et al Genesis. 2006 Jan . 44(1):23-8.

    Inserts

    M2rtTA
    pgk-puro
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pAM5409

    Plasmid

    #132662

    Purpose

    RSF1010-based broad host-range plasmid with RK2 bom site expressing YFP and Sp/Sm resistance

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Inserts

    aadA
    YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PaadA and PconII

    Available since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag p21 WT

    Plasmid

    #16240

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Mar . 3(3):245-52.

    Insert

    p21 (CDKN1A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pN7HELIX

    Plasmid

    #190662

    Purpose

    Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    human codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    CMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3/BLM

    Plasmid

    #111766

    Purpose

    To express human BLM protein in human cells

    Depositor

    Ian Hickson

    Article

    Gaymes et al Oncogene. 2002 Apr 11;21(16):2525-33. do

    Insert

    BLM (BLM Human)
    

    Use

    Tags

    Flag epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pABE8e-protein

    Plasmid

    #161788

    Purpose

    Rhamnose-inducible expression of 8xHIS-ABE8e in E. coli

    Depositor

    David Liu

    Article

    Huang et al Nat Protoc. 2021 Feb;16(2):1089-1128. do

    Insert

    ABE8e base editor
    

    Use

    Tags

    8xHIS and BPNLS

    Expression

    Bacterial

    Mutation

    Promoter

    pRhaBAD

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221_SLC39A1

    Plasmid

    #132058

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC39A1 (SLC39A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCSII-EF/mt-(n1)StayGold

    Plasmid

    #185823

    Purpose

    A soluble photostable fluorescent marker of the mitochondrial matrix. The StayGold gene has codon optimization for mammalian expression.

    Depositor

    Atsushi Miyawaki

    Article

    Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

    Insert

    mt-(n1)StayGold
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR221_EGFP

    Plasmid

    #25899

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    EGFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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