163,637 results

1. Mitochondrial cpGFP

   Plasmid

   #215711

   Purpose

   Expresses cpGFP in the mitochondria

   Depositor

   Katharine Diehl

   Article

   Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

   Insert

   cpGFP
   

   Use

   Lentiviral

   Tags

   FLAG, HA, and mitochondrial localization sequence…

   Expression

   Mammalian

   Mutation

   circularly permuted GFP

   Promoter

   CMV

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV2/9 REP-AAP-ΔCAP

   Plasmid

   #205991

   Purpose

   Rep and AAP protein expression for AAV CREATE libraries

   Depositor

   Viviana Gradinaru

   Article

   Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

   Insert

   AAV9 Rep and AAP proteins for CREATE libraries
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-TetO-hNGN2-Neo

   Plasmid

   #99378

   Purpose

   Expresses human NEUROGENIN2 (hNGN2) and neomycin resistance gene under control of TetON promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   hNGN2-P2A-Neo (NEUROG2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF2944

   Plasmid

   #144420

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SATB2 (SATB2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJT305 Pa01 Lenti pEF-attB LSR GFP

   Plasmid

   #184948

   Purpose

   Lentiviral delivery of recombinase landing pad

   Depositor

   Patrick Hsu, Ami Bhatt, Michael Bassik, Lacra Bintu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Pa01 Lenti pEF1a-attB LSR GFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pABE8e-protein

   Plasmid

   #161788

   Purpose

   Rhamnose-inducible expression of 8xHIS-ABE8e in E. coli

   Depositor

   David Liu

   Article

   Huang et al Nat Protoc. 2021 Feb;16(2):1089-1128. do

   Insert

   ABE8e base editor
   

   Use

   Tags

   8xHIS and BPNLS

   Expression

   Bacterial

   Mutation

   Promoter

   pRhaBAD

   Available Since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpCas9-CBE6b-V106W

   Plasmid

   #215825

   Purpose

   Express CBE6b V106W (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6b V106W-SpCas9-2xUGI (cas9 )
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCF806-shABC1

   Plasmid

   #186713

   Purpose

   Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC1 = shARAF.381-shBRAF.2015-shCRAF.2446). The shABC2 set is better than shABC1.

   Depositor

   Christof Fellmann

   Article

   Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

   Insert

   ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-mEGFP(A206K)-paCaMKII

   Plasmid

   #165438

   Purpose

   Expresses photoactivatable CaMKII in mammalian cells to induce synaptic plasticity.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   mEGFP(A206K)-paCaMKII
   

   Use

   Tags

   mEGFP(A206K)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF2031

    Plasmid

    #142906

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PRDM1 (PRDM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-ArchT-BFP2-TSERex

    Plasmid

    #123312

    Purpose

    Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

    Depositor

    Yulong Li

    Article

    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

    Insert

    ArchT-BFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. CaVα2δ1

    Plasmid

    #26575

    Depositor

    Diane Lipscombe

    Article

    Lin et al J Neurophysiol. 2004 Nov . 92(5):2820-30

    Insert

    Cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B

    Plasmid

    #106309

    Purpose

    The plasmid utilizes RT3 sequence to minimize the expression of 3xHaloTag (Methionine mutated to Leucine)-H2B for long-term H2B single-molecule imaging.

    Depositor

    James Zhe Liu

    Article

    Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

    Insert

    6xflag-RT3-3xHaloTag(MtoL)-H2B (H2BC3 Human)
    

    Use

    Tags

    6xflag-3xHaloTag(MtoL)

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. V5-GFP-APEX2-NES_pLX208

    Plasmid

    #202018

    Purpose

    expresses GFP-APEX2 in the mammalian cytosol, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    V5-GFP-APEX2-NES
    

    Use

    Lentiviral

    Tags

    GFP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. scAAV-Cre

    Plasmid

    #177933

    Purpose

    Expresses Cre-recombinase and can be packaged into AAV particles.

    Depositor

    Roland Rad

    Article

    Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

    Insert

    CAG-Cre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF0912

    Plasmid

    #143449

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF831 (ZNF831 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCXLE-hGLIS1

    Plasmid

    #37625

    Purpose

    Non-integrating (episomal) expression of human GLIS1

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    human GLIS1 (GLIS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T110A, A187G, I221T

    Promoter

    CAG

    Available Since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBT279

    Plasmid

    #122609

    Purpose

    express FERNY-BE4max in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    FERNY-BE4max
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. EB1-tdTomato

    Plasmid

    #50825

    Purpose

    expresses EB1-tdTomato fusion in mammalian cells for labeling the growing tips of MTs

    Depositor

    Erik Dent

    Insert

    EB1 (Mapre1 Mouse)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)-GRP78/BiP

    Plasmid

    #32701

    Depositor

    Richard C. Austin

    Article

    Werstuck et al J Clin Invest. 2001 May;107(10):1263-73.

    Insert

    GRP78/BiP (HSPA5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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