166,034 results

1. pHR-EF1Alpha-Tet-on 3G

   Plasmid

   #118592

   Purpose

   Expresses Tet-on 3G from the EF1Alpha promoter

   Depositor

   Stanley Qi

   Article

   Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

   Insert

   Tet-on 3G
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2

   Plasmid

   #219548

   Purpose

   NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

   Depositor

   Vladislav Verkhusha

   Article

   Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

   Insert

   NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2
   

   Expression

   Mammalian

   Mutation

   The (SAGG)x4 linker between GAL4(1-65) and the PC…

   Promoter

   CMVd1

   Available Since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCW-Flag-CtIP-T847E

   Plasmid

   #71110

   Purpose

   mammalian expession of Flag-CtlP T847E mutation

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   CtlP
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T847E

   Promoter

   TRE promoter, Tet ON

   Available Since

   Dec. 9, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pKS021 - pCAGGS-3XFLAG-mKate2-SA1-bpA

   Plasmid

   #156440

   Purpose

   For transient expression of mouse SA1 (= STAG1 or SCC3A) tagged with mKate2

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   mouse SA1 (Stag1 Mouse)
   

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CRY2olig-GP

   Plasmid

   #218918

   Purpose

   Expression of CRY2olig-mCherry fused to a codon-optimised poly-GP sequence

   Depositor

   Tatyana Shelkovnikova

   Article

   Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

   Insert

   CRY2olig-mCherry tagged poly-GP
   

   Expression

   Mammalian

   Mutation

   wild-type

   Promoter

   CMV

   Available Since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET21a-Traptavidin

   Plasmid

   #24753

   Depositor

   Mark Howarth

   Article

   Chivers et al Nat Methods. 2010 May;7(5):391-3. doi: 1

   Insert

   Traptavidin
   

   Tags

   His-6

   Expression

   Bacterial

   Mutation

   Streptavidin with 2 amino acid mutations: S52G an…

   Available Since

   June 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pVasa-Cre

   Plasmid

   #15885

   Depositor

   Diego Castrillon

   Article

   Gallardo et al Genesis. 2007 Jun . 45(6):413-7.

   Insert

   mouse vasa promoter (Ddx4 Mouse)
   

   Tags

   Cre

   Expression

   Mammalian

   Available Since

   Aug. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pWXC078

   Plasmid

   #222961

   Purpose

   C. elegans codon-optimized mScarlet-I3 with 3 introns cloned into pPD95.75 backbone

   Depositor

   Oliver Hobert

   Article

   Cao et al Genetics. 2024 Oct 7;228(2):iyae126. doi

   Insert

   mScarlet-I3
   

   Expression

   Worm

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TrkB Acceptor

   Plasmid

   #83953

   Purpose

   FRET acceptor for TrkB mEGFP

   Depositor

   Ryohei Yasuda

   Article

   Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

   Insert

   Phospholipase C gamma 1 (Plcg1 Mouse)
   

   Tags

   mRFP

   Expression

   Mammalian

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p101_CRISPRai_PiggyBac

    Plasmid

    #213777

    Purpose

    Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.

    Depositor

    Howard Chang

    Article

    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Inserts

    VPR-dSaCas9
    dSpCas9-KRAB-BFP
    zeocin
    Tet-On transactivator
    

    Use

    CRISPR

    Promoter

    EF1a (EF-1-alpha intron a) and Tet_On_TRE

    Available Since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pmCherry-Synaptojanin-1-145

    Plasmid

    #223563

    Purpose

    Expresses the short isoform of SYNJ1 (neuronal) with mCherry tagged at the n-terminus

    Depositor

    Pietro De Camilli

    Article

    Mohd Rafiq et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    SYNJ1 (SYNJ1 Human)
    

    Tags

    His and mCherry

    Expression

    Mammalian

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLX208-cytoFLARE1.0-TEV

    Plasmid

    #234512

    Purpose

    Transcriptional reporter for detecting calcium increase in HEK293T (TEVp); the same TEVp involved in FLiCRE2

    Depositor

    Wenjing Wang

    Article

    Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

    Insert

    EGFP-P2A-CaM-V5-TEVp
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 11, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. CMV-CRY2oligo-mCherry-ANXA11(186-505 aa)

    Plasmid

    #164208

    Purpose

    Expresses CRY2oligo-mcherry tagged ANXA11 C terminal under CMV promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Tags

    CRY2oligo-mcherry

    Expression

    Mammalian

    Mutation

    186-505 aa

    Promoter

    CMV

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CMV-CRY2oligo-mcherry-ANXA11(1-185aa)

    Plasmid

    #164207

    Purpose

    Expresses CRY2oligo-mcherry tagged ANXA11 N terminal under CMV promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Tags

    CRY2oligo-mcherry

    Expression

    Mammalian

    Mutation

    1-185 aa

    Promoter

    CMV

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-2xFLAG-2xSTREP-CDK6

    Plasmid

    #172614

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDK6 (CDK6 Human)
    

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-DN-hCUL2-FLAG

    Plasmid

    #15819

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005;399:287-309. doi:

    Insert

    Cullin2 (CUL2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N427

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAT201

    Plasmid

    #134878

    Purpose

    Bacterial expression

    Depositor

    Thomas Howard

    Article

    Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pF155 pcDNA3.1(+)SOD1G93A

    Plasmid

    #26401

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Expression

    Mammalian

    Mutation

    G93A

    Available Since

    Nov. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-Bcl2

    Plasmid

    #195551

    Purpose

    Expresses Bcl2 in mammalian cells

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Bcl2 (Bcl2 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Promoter

    CMV enhancer and promoter

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTS118 His14-Avi-28xGS-anti ALFA tag nanobody

    Plasmid

    #199371

    Purpose

    Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    anti-ALFA nanobody
    

    Tags

    14xHis-Avi

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T5-LacO

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge