159,371 results

1. HiBiT-FZD8

   Plasmid

   #195846

   Purpose

   Expresses human FZD8 with an N-terminal HiBiT

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD8 (FZD8 Human)
   

   Use

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBbS8c-ddcpf1-rfp

   Plasmid

   #153038

   Purpose

   For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   Ascpf1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E993A

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-C1-CAMKIIalpha

   Plasmid

   #21226

   Depositor

   Tobias Meyer

   Article

   Shen et al Neuron. 1998 Sep . 21(3):593-606.

   Insert

   CAMKIIalpha (Camk2a Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Lab plasmid KS 0001.

   Promoter

   Available since

   July 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE

   Plasmid

   #130995

   Purpose

   AAV vector to drive the expression of soma-targeted ChRmine-mScarlet under the control of human synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Has Service

   AAV8

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   mScarlet-Kv2.1

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNatB (pACYCduet-naa20-naa25)

   Plasmid

   #53613

   Purpose

   Allows expression of the fission yeast NatB complex - chloramphenicol marker

   Depositor

   Dan Mulvihill

   Article

   Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

   Inserts

   naa20 (naa20 Fission Yeast)
   naa25 (naa25 Fission Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TFORF3464

   Plasmid

   #144940

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   KLF7 (KLF7 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXG237

   Plasmid

   #141281

   Purpose

   ATF4 translational reporter

   Depositor

   Martin Kampmann

   Article

   Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

   Insert

   uORF1/2 of ATF4 followed by mApple
   

   Use

   Lentiviral

   Tags

   mApple

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE30-His-AlaRS

   Plasmid

   #124103

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Alanyl-tRNA ligase (alaS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Myosin-IIA-GFP

   Plasmid

   #38297

   Depositor

   Matthew Krummel

   Article

   Jacobelli et al J Immunol. 2009 Feb 15;182(4):2041-50.

   Insert

   Myosin II A (Myh9 Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC18T-mini-Tn7T-Gm-eyfp

    Plasmid

    #65031

    Purpose

    GmR mini-Tn7 vector, mobilizable, for YFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    eyfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAG1188 mCherry-cagedFIREtag

    Plasmid

    #205177

    Purpose

    Expresses mCherry-cagedFIREtag in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    mCherry-FIREtag-DEVDG-MoA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV.U6.shRNA-Sdc1-1376

    Plasmid

    #195574

    Purpose

    Expresses shRNA to knockdown mouse Syndecan-1

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    shRNA, eGFP (Sdc1 Synthetic)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3 RLUC POLIRES FLUC

    Plasmid

    #45642

    Purpose

    Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

    Depositor

    Nahum Sonenberg

    Article

    Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

    Insert

    RLUC - IRES - FLUC
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p3015b

    Plasmid

    #209317

    Purpose

    Expresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.

    Depositor

    Thomas Hooven

    Article

    Gopalakrishna et al Commun Biol. 2023 Jun 9;6(1):620. doi: 1

    Insert

    Group B Streptococcus-compatible single guide RNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Xyl/tet promoter that is constitutively active

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-ERBB3-V5/HIS

    Plasmid

    #201104

    Purpose

    expression of human ERBB3 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human ERBB3 receptor tyrosine kinase, full length, wildtype (ERBB3 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. (294) pcDNA3.1-myc-OGA(1-400)

    Plasmid

    #168095

    Purpose

    Myc tagged truncated human OGA catalytic domain of residues 1-400

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    myc-OGA(1-400)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE mCherry-Rab11A

    Plasmid

    #228035

    Purpose

    Mammalian lentiviral expression of mCherry-labeled Rab11.

    Depositor

    Wade Harper

    Insert

    Rab11A (RAB11A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPAmCherry3-C1

    Plasmid

    #31933

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M18L/A58T/K69N/L84F/M137I/E146D/S148L/I165L/Q167A…

    Promoter

    CMV

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TFORF1447

    Plasmid

    #143278

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR5A2 (NR5A2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTF-lacZ-rfp

    Plasmid

    #153037

    Purpose

    CRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    CRISPR array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge