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(Plasmid #45482)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 45482 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pcDNA4/TO (modified)
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 11000
  • Vector type
    Mammalian Expression ; Tetracycline Inducible
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
  • Alt name
    transient receptor potential cation channel, subfamily M, member 7
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • GenBank ID
    NM_021450.2 NP_067425.2
  • Entrez Gene
    Trpm7 (a.k.a. 2310022G15Rik, 4833414K03Rik, 5033407O22Rik, CHA, CHAK, CHAK1, LTR, LTrpC-7, Lt, Ltpr7, Ltrpc7, TRP, TRPPLIK)
  • Promoter Tetracycline-controlled CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer LNCX; CMV-F
  • 3′ sequencing primer BGH-Rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

For the purpose of expressing LTRPC7 in eukaryotic cells, the depositing lab used PCR to produce an epitope tagged expression construct from two overlapping murine LTRPC7 clones. The LTRPC7 coding sequence was modified by removing the initiating methionine and replacing it with a sequence encoding a Kozak sequence, the FLAG tag and the additional sequence GCGGCCGCAT, and by placing a SpeI site just after the stop codon. These modifications result in an expressed protein which started with the following amino acid sequence: MGDYKDDDDKRPH followed by the murine LTRPC7 coding sequence starting at the second amino acid. This construct is expressed from the pcDNA4/TO vector which provides tetracycline-controlled expression from a CMV promotor.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4/TO mTRPM7 was a gift from Andrew Scharenberg (Addgene plasmid # 45482 ; ; RRID:Addgene_45482)
  • For your References section:

    LTRPC7 is a Mg.ATP-regulated divalent cation channel required for cell viability. Nadler MJ, Hermosura MC, Inabe K, Perraud AL, Zhu Q, Stokes AJ, Kurosaki T, Kinet JP, Penner R, Scharenberg AM, Fleig A. Nature. 2001 May 31;411(6837):590-5. 10.1038/35079092 PubMed 11385574