159,371 results

1. eGFP-MYO5B

   Plasmid

   #239265

   Purpose

   Expression of N terminal GFP tagged Myo5B

   Depositor

   David Pellman

   Article

   Zhao et al Dev Cell. 2023 May 22;58(10):847-865.e10

   Insert

   MYO5B (MYO5B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-26b-NbVHH05-HA-His

   Plasmid

   #171567

   Purpose

   Bacterial expression of HA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-HA-His)

   Depositor

   Hidde Ploegh, Norbert Perrimon

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   NbVHH05-HA-His (UBE2J1 Human)
   

   Use

   Tags

   HA-tag/His-tag and PelB signal peptide

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. EF1a_mCherry_P2A_Hygro

   Plasmid

   #135003

   Purpose

   Lentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAS_4xMma PylT_FLAG-AbKRS-chIPYE

   Plasmid

   #140020

   Purpose

   Plasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   AbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…

   Promoter

   EF1

   Available since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. MTS-mCherry-GFP1-10-Hyg-N1

   Plasmid

   #91957

   Purpose

   Expresses mCherry-GFP1-10 in mammalian cell mitochondria

   Depositor

   Rong Li

   Article

   Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

   Insert

   MTS-mCherry-GFP1-10
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA1-Tat

   Plasmid

   #138478

   Purpose

   Expresses HIV Tat for efficient sgRNA packaging.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   Tat
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. p3xFLAG-CMV10-hFIP200

   Plasmid

   #24300

   Depositor

   Noboru Mizushima

   Article

   Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

   Insert

   FIP200 (RB1CC1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-ABEmax-SpRY

   Plasmid

   #195279

   Purpose

   Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) without EGFP

   Depositor

   Carsten Lederer, Petros Patsali

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpRY without EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. P427-FBP_13

   Plasmid

   #139704

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtug7, pUBQ1:NhH3H:tNos, p35S:NnHisps:t35S, pAtELF4:NnLuz:trbcse9
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   various

   Available since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVP143

    Plasmid

    #222080

    Purpose

    Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and AeBlue for plasmid eviction.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1

    Insert

    dCas9-PmCDA1, sfGFP, sacB and AeBlue
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. ZIF-NLS-EGFP*-HOTag6-T2A-CEL-EGFP-YAP

    Plasmid

    #217611

    Purpose

    Lentiviral vector for constitutive expression of YAP/SPARK-ON (SFFV promoter)

    Depositor

    Xiaokun Shu

    Article

    Chung et al ACS Cent Sci. 2023 Jul 7;9(7):1466-1479.

    Insert

    ZIF-NLS-EGFP*-HOTag6-T2A-CEL-EGFP-YAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTECH-chAcK3RS(IPYE)

    Plasmid

    #104069

    Purpose

    Expression plasmid encoding chAcK3RS(IPYE) and pylT

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Plpp chAcK3RS(IPYE); PproK pyl
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains activating mutations V31I, T56P, H62Y, A…

    Promoter

    Plpp and PproK

    Available since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-HA-deLACCO1-NGR

    Plasmid

    #208024

    Purpose

    Control biosensor for extracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    deLACCO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBIG1a

    Plasmid

    #80611

    Purpose

    pBIG1a - Step1 vector a (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. OMMGFP 1-10

    Plasmid

    #164119

    Purpose

    Expresses the non-fluorescent GFP1-10 moiety on the cytosolic face of the OMM

    Depositor

    Marisa Brini, Tito Calì

    Article

    Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

    Insert

    OMMGFP 1-10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMRX-IP-TMEM192-mKeima

    Plasmid

    #215358

    Purpose

    Probe to evaluate lysophagic activity

    Depositor

    Tamotsu Yoshimori

    Article

    Shima et al J Cell Biol. 2023 Dec 4;222(12):e2022040

    Insert

    transmembrane protein 192 (TMEM192 Human)
    

    Use

    Retroviral

    Tags

    mKeima

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 Flag-HIF1B (#930)

    Plasmid

    #99916

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Chen et al Nature. 2016 Nov 3;539(7627):112-117. do

    Insert

    HIF1B (ARNT Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21a-SerRS-His

    Plasmid

    #124118

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Seryl-tRNA ligase (serS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET StrepII-StrepII TEV LIC cloning vector (2RRT)

    Plasmid

    #55215

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    2xStrep II and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1_RFP

    Plasmid

    #206177

    Purpose

    mammalian expression of RFP

    Depositor

    Christopher Goodnow

    Article

    Russell et al Proc Natl Acad Sci U S A. 2021 Aug 24;11

    Insert

    RFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge