166,339 results

1. pGEX6P1-N-His

   Plasmid

   #119755

   Purpose

   Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal 10x His tag.

   Depositor

   Andrew Jackson, Martin Reijns

   Type

   Empty backbone

   Tags

   10xHis and GST

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO-Tet-On-YAP1-shRNA1

   Plasmid

   #176846

   Purpose

   lentiviral vector for doxycycline-inducible human YAP1 knockdown

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   YAP1-Sh1 (YAP1 Human)
   

   Use

   Lentiviral

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti6-GFP

   Plasmid

   #35637

   Depositor

   Daniel Haber

   Article

   Singh et al Cell. 2012 Feb 17;148(4):639-50.

   Insert

   GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 26, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pCXN2-BCL6 (CXN-BCL6)

   Plasmid

   #40346

   Depositor

   Alexander Dent

   Article

   Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

   Insert

   BCL6 (BCL6 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. Human Beta-catenin GFP

   Plasmid

   #71367

   Purpose

   mammalian expression of human Beta-catenin GFP

   Depositor

   Alpha Yap

   Article

   Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

   Insert

   Beta-catenin (CTNNB1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3-Sox2

   Plasmid

   #101761

   Purpose

   Sox2 promoter reporter

   Depositor

   Yuh-Shan Jou

   Article

   Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

   Insert

   SRY-box 2 promoter (SOX2 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVRC8400-SARS-CoV-2-RBD-AVI

   Plasmid

   #160476

   Purpose

   Expresses SARS-CoV-2 RBD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

   Insert

   SARS-CoV-2-RBD
   

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Available Since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-puro-CMV-VC3AI

   Plasmid

   #78907

   Purpose

   Indicator of caspase-3-like protease activity

   Depositor

   Binghui Li

   Article

   Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

   Insert

   VC3AI
   

   Use

   Lentiviral

   Tags

   HA and Myc

   Mutation

   S320A, deletion of AA1-200

   Promoter

   CMV

   Available Since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET24c(+)-EFTu

   Plasmid

   #31172

   Depositor

   Linda Spremulli

   Article

   Zhang et al FEBS Lett. 1996 Aug 12. 391(3):330-2.

   Insert

   E. coli elongation factor Tu (tufB E. coli)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pCDH-CMV

    Plasmid

    #72265

    Purpose

    Express gene of interest under CMV promoter

    Depositor

    Kazuhiro Oka

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 25, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. mRuby3-C1

    Plasmid

    #127808

    Purpose

    mRuby3 C1 Cloning vector

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

    Insert

    mRuby3
    

    Expression

    Mammalian

    Available Since

    June 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO_LAMP1_HaloTag9

    Plasmid

    #175541

    Purpose

    Lyzosomal expression of HaloTag9 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    LAMP1-HaloTag9
    

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pwB3b

    Plasmid

    #32106

    Depositor

    Christopher Buck

    Article

    Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

    Inserts

    BKV VP1
    BKV VP3
    

    Expression

    Mammalian

    Mutation

    codon modified

    Available Since

    Oct. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO_CalR_HaloTag9_KDEL

    Plasmid

    #175540

    Purpose

    Expression of HaloTag9 in the endoplasmic reticulum of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    CalR-HaloTag9-KDEL
    

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJMP1337

    Plasmid

    #119270

    Purpose

    for cloning new sgRNAs

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. PGK plasmid

    Plasmid

    #169744

    Purpose

    Unregulated "open-loop" PGK promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    PGK promoter

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSAM_AraC

    Plasmid

    #91569

    Purpose

    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.

    Depositor

    Harry Mobley

    Article

    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

    Insert

    AraC/PBAD
    

    Expression

    Bacterial

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT3TS-Tol2

    Plasmid

    #31831

    Depositor

    Stephen Ekker

    Article

    Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

    Insert

    Tol2 Transposase
    

    Use

    Cloning vector

    Promoter

    Lac

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL4-UPRE-luc2P-Hygro

    Plasmid

    #101788

    Purpose

    Luciferase reporter plasmid containing three tandem repeats of the unfolded protein response element (UPRE)

    Depositor

    Seiichi Oyadomari

    Article

    Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

    Insert

    3xUPRE
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Promoter

    minimal TATA-box promoter with low basal activity

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAP01

    Plasmid

    #59981

    Purpose

    The function of this material is to facilitate the capture and expression of bacterial gene clusters.

    Depositor

    Bradley Moore

    Article

    Yamanaka et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

    Type

    Empty backbone

    Use

    Synthetic Biology; Transformation-associated reco…

    Tags

    no

    Expression

    Bacterial and Yeast

    Promoter

    no

    Available Since

    Nov. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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