161,813 results

1. pAL119-TK

   Plasmid

   #21911

   Depositor

   Maria Castro

   Article

   Dewey et al Nat Med. 1999 Nov . 5(11):1256-63.

   Insert

   HSV Type 1 thymidine kinase
   

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF1983

   Plasmid

   #143562

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TBX5 (TBX5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. N1-Tq-Ca-FLITS

   Plasmid

   #191454

   Purpose

   Genetically encoded calcium sensor (GECI) Tq-Ca-FLITS that reports with lifetime and intensity changes

   Depositor

   Dorus Gadella

   Article

   van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

   Insert

   Tq-Ca-FLITS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRVV669

   Plasmid

   #108283

   Purpose

   Donor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9

   Depositor

   Richard Mann

   Article

   Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

   Insert

   PhiC31 attP sites
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBI-MCS-EYFP

   Plasmid

   #58855

   Purpose

   The tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFP

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   enhanced yellow fluorescent protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-BEC enhancer-minBG-iCre(R279T)-4x6T

   Plasmid

   #236727

   Purpose

   Brain Endothelial Cell Enhancer with 4x6T miRNA targeting cassette For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors.

   Depositor

   Xiangmin Xu

   Article

   Velazquez-Rivera et al Neuron. 2025 May 21;113(10):1562-1578.e6

   Inserts

   cis-regulatory elements 89763
   iCre(R297T)
   4x6T miRNA targeting cassette
   

   Use

   AAV

   Tags

   Expression

   Mutation

   R297T

   Promoter

   Available since

   July 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-HaloAKAR2.2-T2A-EGFP

   Plasmid

   #216490

   Purpose

   Expression of chemigenetic PKA biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

   Depositor

   Jin Zhang

   Article

   Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

   Insert

   HaloAKAR2.2
   

   Use

   Tags

   T2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAcBac3-EcLeu TAG-EGFP**

   Plasmid

   #228785

   Purpose

   EcLeu aaRS/tRNA system for TAG suppression with EGFP containing two stop codons for dual suppression

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcLeuRS
   EcLtR
   EGFP**
   

   Use

   Baculoviral

   Tags

   Expression

   Mammalian

   Mutation

   TAG suppressor

   Promoter

   Available since

   May 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-hNM2B

   Plasmid

   #236533

   Purpose

   For expression of NM2B (human) in mammalian cells

   Depositor

   Sandra Citi

   Article

   Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

   Insert

   MYH10 (MYH10 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQAT46

    Plasmid

    #223418

    Purpose

    T-DNA vector for Mb2Cas12a-RVRR based mutagenesis for monocot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate ZmUbi1; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-Mb2Cas12a-RVRR-ZmUbi-crRNA scaffold
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQAT43

    Plasmid

    #223415

    Purpose

    T-DNA vector for temperature tolerance LbCas12a-D156R based mutagenesis for monocot plants; TTTV PAM; LbCas12a-D156R and the crRNA were driven by separate ZmUbi1 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-LbCas12a-D156R-ZmUbi-crRNA scaffold
    

    Use

    CRISPR; T-dna vector

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAcBac3-pAAFRS-8xYtR-EGFP-3TAA- 40TGA-152TAG-TEV-3xUAA

    Plasmid

    #228788

    Purpose

    EcTyr aaRS/tRNA system for pAAF incorporation with EGFP containing three stop codons for triple suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcTyrRS
    EcYtR
    EGFP***
    TEV protease
    

    Use

    Baculoviral

    Tags

    Expression

    Mammalian

    Mutation

    TAG suppressor

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAcBac3-EcTrp-TGA-EGFP**

    Plasmid

    #228786

    Purpose

    EcTrp aaRS/tRNA system for TGA suppression with EGFP containing two stop codons for dual suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcTrpRS
    EcWtR
    EGFP**
    

    Use

    Baculoviral

    Tags

    Expression

    Mammalian

    Mutation

    TGA suppressor

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. OSCA1.2-peGFP-N1

    Plasmid

    #228585

    Purpose

    Expresses OSCA1.2-eGFP in mammalian cells

    Depositor

    Huanghe Yang

    Article

    Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

    Insert

    hyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. TMEM63A-peGFP-N1

    Plasmid

    #228590

    Purpose

    Expresses mouse TMEM63A-eGFP in mammalian cells

    Depositor

    Huanghe Yang

    Article

    Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

    Insert

    TMEM63A/CSC1-like protein 1 isoform 1 (Tmem63a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJJB1439

    Plasmid

    #218589

    Purpose

    Express peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniol

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    GES
    

    Use

    Tags

    ePTS1

    Expression

    Yeast

    Mutation

    Erg20(F96W, N127W)

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBa.MARK2-Halo

    Plasmid

    #224414

    Purpose

    Mammalian expression of MARK2

    Depositor

    Marvin Bentley

    Article

    Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

    Insert

    MARK2 (MARK2 Human)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta actin

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. L2383-FRB-GPA-NLuc

    Plasmid

    #222902

    Purpose

    Positive control receptor chain for Nanoluciferase signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-20GS-NanoLuc
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRetroX-TRE3G-IRSp53

    Plasmid

    #221317

    Purpose

    Dox-inducible expression of IRSp53 in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    IRSp53 (Baiap2 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. TACR1-DuET

    Plasmid

    #213385

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    TACR1 (TACR1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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