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pCMVTNT-T7-KPNA5
(Plasmid #26681)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26681 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMVTNT
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4050
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Karyopherin Alpha 5
  • Alt name
    KPNA 5
  • Alt name
    Importin Alpha 6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1620
  • GenBank ID
    NM_002269
  • Entrez Gene
    KPNA5 (a.k.a. IPOA6, SRP6)
  • Tag / Fusion Protein
    • T7 (MASMTGGQQMG) (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SV40 late poly A
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please note that an IS1 transposase sequence was found in the plasmid backbone during Addgene's quality control. The EcoRI digest indicates a sub-population of the stock contains this additional IS1 element. The depositor noted that this sequence should NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMVTNT-T7-KPNA5 was a gift from Bryce Paschal (Addgene plasmid # 26681 ; http://n2t.net/addgene:26681 ; RRID:Addgene_26681)
  • For your References section:

    Karyopherin alpha7 (KPNA7), a divergent member of the importin alpha family of nuclear import receptors. Kelley JB, Talley AM, Spencer A, Gioeli D, Paschal BM. BMC Cell Biol. 2010 . 11():63. 10.1186/1471-2121-11-63 PubMed 20701745