|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26681||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4050
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameKaryopherin Alpha 5
Alt nameKPNA 5
Alt nameImportin Alpha 6
SpeciesH. sapiens (human)
Insert Size (bp)1620
Entrez GeneKPNA5 (a.k.a. IPOA6, SRP6)
/ Fusion Protein
- T7 (MASMTGGQQMG) (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SmaI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SV40 late poly A (Common Sequencing Primers)
Please note that an IS1 transposase sequence was found in the plasmid backbone during Addgene's quality control. The EcoRI digest indicates a sub-population of the stock contains this additional IS1 element. The depositor noted that this sequence should NOT affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMVTNT-T7-KPNA5 was a gift from Bryce Paschal (Addgene plasmid # 26681 ; http://n2t.net/addgene:26681 ; RRID:Addgene_26681)
For your References section:Karyopherin alpha7 (KPNA7), a divergent member of the importin alpha family of nuclear import receptors. Kelley JB, Talley AM, Spencer A, Gioeli D, Paschal BM. BMC Cell Biol. 2010 . 11():63. 10.1186/1471-2121-11-63 PubMed 20701745