|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19930||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5437
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namecyclin-dependent kinase inhibitor 2A p19ARF
SpeciesH. sapiens (human)
Insert Size (bp)426
Entrez GeneCDKN2A (a.k.a. ARF, CDK4I, CDKN2, CMM2, INK4, INK4A, MLM, MTS-1, MTS1, P14, P14ARF, P16, P16-INK4A, P16INK4, P16INK4A, P19, P19ARF, TP16)
/ Fusion Protein
- myc (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Note that there are several isoforms of ARF. Addgene encourages scientists to read the associated paper carefully before requesting this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-myc-ARF was a gift from Yue Xiong (Addgene plasmid # 19930 ; http://n2t.net/addgene:19930 ; RRID:Addgene_19930)
For your References section:ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a locus deletion impairs both the Rb and p53 tumor suppression pathways. Zhang Y, Xiong Y, Yarbrough WG. Cell. 1998 Mar 20. 92(6):725-34. 10.1016/S0092-8674(00)81401-4 PubMed 9529249