pCMV p16 INK4A
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10916||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5446
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namep16 INK4
SpeciesH. sapiens (human)
Insert Size (bp)472
Entrez GeneCDKN2A (a.k.a. ARF, CDK4I, CDKN2, CMM2, INK4, INK4A, MLM, MTS-1, MTS1, P14, P14ARF, P16, P16-INK4A, P16INK4, P16INK4A, P19, P19ARF, TP16)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
A human pl6ink4 cDNA was obtained by PCR amplification of a HeLa cell cDNA library with a 5' primer (5'-GGAATTCACCACCATGGAGCCTTCGGCTGAC-3') and a 3' primer (5'-GGAATTCTCGAGTCAATCGGGGATATCTGAGGGACC-3'). A 472-bp fragment was amplified, purified on a low-melting agarose gel, and cloned directly into pGEM-T (Promega). The resulting plasmid was then used to isolate an EcoRI/Xho I fragment containing the pl6ink4 coding region, which was cloned into pcDNA3 (Invitrogen) to construct pCMV.pl6ink4.
The insert corresponds to isoform 1 but is missing the first 8 amino acids.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV p16 INK4A was a gift from Bob Weinberg (Addgene plasmid # 10916 ; http://n2t.net/addgene:10916 ; RRID:Addgene_10916)
For your References section:Growth suppression by p16ink4 requires functional retinoblastoma protein. Medema RH, Herrera RE, Lam F, Weinberg RA. Proc Natl Acad Sci U S A. 1995 Jul 3. 92(14):6289-93. 10.1073/pnas.92.14.6289 PubMed 7603984