161,851 results

1. pTlpA_mCherry_YafQ/DinJ

   Plasmid

   #229148

   Purpose

   Toxin-Antitoxin (Dinj/YafQ)

   Depositor

   Shrikrishnan Sankaran

   Article

   Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

   Insert

   mCherry, DinJ/YafQ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PtlpA (mCherry), DinJ/YafQ (Native)

   Available since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSFDUET-1_VNp-anti-GFP mAb

   Plasmid

   #214955

   Purpose

   Bacterial expression of anti-GFP mAb light and heavy chain VNp fusions.

   Depositor

   Dan Mulvihill

   Article

   Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

   Inserts

   VNp-anti-GFP mAb heavy chain
   VNp-anti-GFP mAb light chain
   

   Use

   Tags

   VNp6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. PRS_8-jRGECO1a

   Plasmid

   #210399

   Purpose

   AAV-vector for fluorescent calcium measurements in the red spectrum from noradrenergic neurons

   Depositor

   Simon Wiegert

   Article

   Wissing et al PLoS Biol. 2025 Jul 7;23(7):e3003228. do

   Insert

   jRGeco1a
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PRSx8

   Available since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF2209

   Plasmid

   #142175

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CREB3L3 (CREB3L3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pD649-HAsp-PTK7-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156943

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   PTK7 (PTK7 Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiCRISPRv2hyg-Ptgs1

   Plasmid

   #170299

   Purpose

   A knock-out vector for the mouse Ptgs1

   Depositor

   Michiyuki Matsuda

   Article

   Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

   Insert

   A gRNA targeting the mouse Ptgs1 gene.
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGI3EM11

   Plasmid

   #135483

   Purpose

   Agrobacterium T-DNA with SpunH2Bpr driving hph-GFP

   Depositor

   Nicolas Buchler

   Article

   Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

   Insert

   SpunH2Bpr hph-GFP ADH1t
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpunH2Bpr

   Available since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLfa[UAS-Tc'hsp_p-tGFP-SV40]fa

   Plasmid

   #86452

   Purpose

   UAS responder construct for misexpression in Tribolium; UAS drives tGFP expression; (Shuttle plasmid); tGFP can be exchanged by other ORFs

   Depositor

   Gregor Bucher

   Insert

   UAS-Tc'hsp_p-tGFP-SV40
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS-Int

   Plasmid

   #50000

   Purpose

   Expresses the integrase on a suicide plasmid

   Depositor

   Brian Robertson, Peter Sander

   Article

   Springer et al Int J Med Microbiol. 2001 Mar;290(8):669

   Insert

   Integrase from pMV361
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   own

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pET-29b(+)_His-CBD-SpEndoH

    Plasmid

    #218196

    Purpose

    Bacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH

    Depositor

    Florence Pojer

    Insert

    EndoH
    

    Use

    Tags

    tufA-His-CBD

    Expression

    Bacterial

    Mutation

    codon optimized Ecoli, residues 45-313

    Promoter

    T7

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF0500

    Plasmid

    #143709

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PPARGC1A (PPARGC1A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. PRKAG3 gRNA (BRDN0001145578)

    Plasmid

    #77295

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAG3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAG3 (PRKAG3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. PRKAG3 gRNA (BRDN0001147132)

    Plasmid

    #77296

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAG3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAG3 (PRKAG3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. PRKAG3 gRNA (BRDN0001146704)

    Plasmid

    #77294

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAG3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAG3 (PRKAG3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. IF-GFP-ATRX

    Plasmid

    #45444

    Purpose

    ATRX expression vector

    Depositor

    Michael Dyer

    Article

    Valle-Garcia et al Springerplus. 2014 May 2;3:222. doi: 10.

    Insert

    ATRX (ATRX Mouse, Human)
    

    Use

    Tags

    GFP and HA

    Expression

    Bacterial and Mammalian

    Mutation

    isoform 2, missing codon E124

    Promoter

    PCMV

    Available since

    May 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hChATp-tdTomato

    Plasmid

    #197564

    Purpose

    Expresses tdTomato under a human choline acetyltransferase (hChAT) promoter fragment

    Depositor

    Casey Maguire

    Article

    Santoscoy et al Mol Ther Methods Clin Dev. 2023 May 9;29

    Insert

    tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human ChAT promoter

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX6P1-N-FLAG

    Plasmid

    #119759

    Purpose

    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal FLAG tag.

    Depositor

    Andrew Jackson, Martin Reijns

    Type

    Empty backbone

    Use

    Tags

    FLAG and GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLminP_Luc2P_RE54

    Plasmid

    #90399

    Purpose

    C/EBPa - CEBPA gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    STAT1

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAS088

    Plasmid

    #211502

    Purpose

    AAV genome backbone for AAV-Perturb-seq with direct gRNA capture and nuclei sorting based on eGFP.

    Depositor

    Randall Platt

    Article

    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

    Type

    Empty backbone

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSG5 PPAR alpha

    Plasmid

    #22751

    Depositor

    Bruce Spiegelman

    Insert

    PPARalpha (Ppara Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge