164,223 results

1. pHBS1502 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaCTD]

   Plasmid

   #133326

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of NTD in full-length TDP43

   Promoter

   Available Since

   Nov. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-Rab11-DN (S25N)

   Plasmid

   #101046

   Purpose

   Expresses HA-tagged human Rab11-DN (S25N) in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Article

   Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

   Insert

   RAB11 (RAB11A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S25N

   Promoter

   CMV

   Available Since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCasY1

   Plasmid

   #87687

   Purpose

   p15A plasmid expressing CasY1

   Depositor

   Jennifer Doudna

   Article

   Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

   Insert

   CasY1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-Ef1a-InteinC-CreC

   Plasmid

   #187615

   Purpose

   AAV vector encoding C-terminal portion of split-Intein-mediated split-Cre

   Depositor

   Il Hwan Kim

   Article

   Kim et al Cell Rep. 2022 Jun 7;39(10):110906. doi:

   Insert

   InteinC-CreC
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCI-neo Flag HAUSP

   Plasmid

   #16655

   Depositor

   Bert Vogelstein

   Article

   Cummins et al Cell Cycle. 2004 Jun . 3(6):689-92.

   Insert

   HAUSP (USP7 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pF1-Hs Cdk1/cyclin B1/Cks1

   Plasmid

   #177329

   Purpose

   Co-expression of the human active Cdk1/cyclin B1/Cks1 kinase complex

   Depositor

   Andreas Boland

   Article

   Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

   Inserts

   Cyclin-dependent kinase 1 (CDK1 Human)
   G2/mitotic-specific cyclin-B1 (CCNB1 Human)
   Cyclin-dependent kinases regulatory subunit 1 (CKS1B Human)
   

   Use

   Tags

   2 x Strep-tag and 8 x His

   Expression

   Insect

   Mutation

   Promoter

   p10 and polh

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSD141B

   Plasmid

   #218139

   Purpose

   Lentiviral vector expressing evolved degron SD56 fused to eGFP with mCherry control.

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   SD56-eGFP-IRES2-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUCM-CLYBL-hNIL

   Plasmid

   #105841

   Purpose

   Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron

   Depositor

   Michael Ward

   Article

   Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

   Inserts

   Neurogenin 2 (NEUROG2 Human)
   ISL LIM Homeobox 1 (ISL1 Human)
   LIM Homeobox 3 (LHX3 Human)
   mCherry
   rtTA3G
   

   Use

   CRISPR and TALEN

   Tags

   Expression

   Mutation

   Promoter

   CAG, EF-1alpha, and TRE3G

   Available Since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-OsTIR1(F74G)

   Plasmid

   #140730

   Purpose

   OsTIR1(F74G)-P2A-mAID-EGFP-NES

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   OsTIR1(F74G)-P2A-mAID-EGFP-NES
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-GFP-TFE3pA

    Plasmid

    #120201

    Purpose

    Human TFE3 poly(A) site reporter

    Depositor

    David Bartel

    Article

    Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

    Insert

    TFE3 poly(A) site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYZ038

    Plasmid

    #200227

    Purpose

    universal template to build tRNA-gRNA array for multiplex editing CREEPY

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    tRNA-gRNA scaffold
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Flag-FOXP3

    Plasmid

    #153147

    Purpose

    Expression of Flag tagged human FOXP3 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXP3 (FOXP3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PTK2 gRNA (BRDN0001146703)

    Plasmid

    #75544

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PTK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PTK2 (PTK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-EFS-MCP-HALOnls

    Plasmid

    #121937

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    MCP-HALOnls
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pgk-ATG-frt

    Plasmid

    #20734

    Depositor

    Rudolf Jaenisch

    Article

    Beard et al Genesis. 2006 Jan . 44(1):23-8.

    Insert

    pgk-ATG-frt-SAdpA-tetO
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. LeGO-G2

    Plasmid

    #25917

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    SFFV promoter, eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBP-split-intein-Gal4[21-881]-C-int

    Plasmid

    #199202

    Purpose

    Gateway destination vector for enhancer-driven split-intein Gal4[21-881]C-int expression

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    split-intein-Gal4[21-881]-C-int
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET15b-His-IkappaBalpha(FL)

    Plasmid

    #44738

    Depositor

    Gourisankar Ghosh

    Insert

    IkappaBalpga (Nfkbia Mouse)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

19. PTK2 gRNA (BRDN0001148426)

    Plasmid

    #75545

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PTK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PTK2 (PTK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE2_EF1a_TetOn_IRES_mCherry

    Plasmid

    #204155

    Purpose

    Lentivirus backbone expressing rtTA linked to mCherry

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    Tet-On 3G
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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