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pEGFP-N1-FLAG

Plasmid

#60360

Purpose

Plasmid for cloning and expression of FLAG-GFP tagged proteins (C-terminal tag). Confers resistance to G418.

Depositor

Sébastien Britton, Patrick Calsou

Article

Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

EGFP and FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcs2+spCas9 ABE-Umax-nanos

Plasmid

#222142

Purpose

Adenine base editor with modified TadA variant for germline A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9 with nanos UTR

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Promoter

SP6

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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mPapaya-pBAD

Plasmid

#54838

Purpose

Localization: Protein Expression Vector , Excitation: 530, Emission: 541

Depositor

Robert Campbell, Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Type

Empty backbone

Tags

6xHis and mPapaya

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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pET21a-MTF-His

Plasmid

#124133

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Methionyl-tRNA formyltransferase (fmt Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMTTH+H1.4

Plasmid

#157796

Purpose

Expression of human linker histone H1.4

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H1.4 (H1-4 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27B

Plasmid

#89447

Purpose

Expression of GFP-tagged human Rab27B in mammalian cells

Depositor

Wendy Westbroek

Article

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Insert

human Rab27B (RAB27B Human)

Tags

eGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGS-BacA-21222-SIN3A-3xFLAG

Plasmid

#109213

Purpose

Encodes human full-length SIN3A with a C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Jayaprakash et al Mol Biotechnol. 2021 Jan;63(1):53-62. do

Insert

human full-length SIN3A (SIN3A Human)

Tags

3xFLAG

Expression

Insect

Promoter

Polyhedrin

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CRE-CytoTape-V5

Plasmid

#239430

Purpose

CytoTape signal monomer for recording phosphorylated CREB protein activity

Depositor

Changyang Linghu

Article

Zheng et al Nature. 2026 Jan 26. doi: 10.1038/s41586

Insert

CytoTape-V5

Use

AAV

Tags

V5-dMBP

Expression

Mammalian

Promoter

CRE promoter

Available Since

Nov. 12, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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murine TCR OTII-2A.pMIG II

Plasmid

#52112

Purpose

retroviral expression of both TCRalpha and TCRbeta using a single vector

Depositor

Dario Vignali

Article

Holst et al Nat Protoc. 2006;1(1):406-17.

Inserts

T cell receptor alpha chain (Tcra Mouse)
T cell receptor beta chain (Tcrb Mouse)

Use

Retroviral

Tags

P2A (from porcine teschovirus-1)

Expression

Mammalian

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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lenti SYN-dCas9-KRAB-MeCP2

Plasmid

#155365

Purpose

Expresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoter

Depositor

Jeremy Day

Article

Duke et al Front Genome Ed. 2020 Sep 15;2:9. doi: 1

Insert

dCas9-KRAB-MeCP2

Use

CRISPR and Lentiviral

Tags

3x FLAG and 7x His

Expression

Mammalian

Promoter

SYN

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pMK232 (CMV-OsTIR1-PURO)

Plasmid

#72834

Purpose

Expresses OsTIR1 under the control of the CMV promoter

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

AAVS1-CMV-OsTIR1-PURO-AAVS1

Expression

Mammalian

Available Since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CamKII 0.4.Cre.SV40

Plasmid

#105558

Purpose

AAV expression of Cre from CamKII promoter

Depositor

James M. Wilson

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

Cre

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CamKII

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBITE-CD19-CD3

Plasmid

#190677

Purpose

Encodes a modularized form of HD37-OKT3 bispecific antibody

Depositor

Scott McComb

Article

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Modularized-HD37-modularized-OKT3-P2A-NeonGreen

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

EFS

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-PTEN

Plasmid

#13039

Depositor

Alonzo Ross

Article

Liu et al J Cell Biochem. 2005 Oct 1. 96(2):221-34

Insert

PTEN (PTEN Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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gag-MCP-pol

Plasmid

#211370

Purpose

Packaging plasmid for v3 PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MMLVgag-MCP-pol

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRL1342

Plasmid

#70691

Purpose

RSF1010-based vector with extensive polylinker, and resistance to Cm and Em. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.

Depositor

Peter Wolk

Article

Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.

Type

Empty backbone

Use

Bacterial shuttle vector

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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mEmerald-Desmin-C-18

Plasmid

#54059

Purpose

Localization: Intermediate Filaments, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Desmin (DES Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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PEP101E

Plasmid

#97387

Purpose

binary construct of cytosolic sfGFP1-10 for plant

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Expression

Plant

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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TFORF2344

Plasmid

#142932

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MITF (MITF Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMK382

Plasmid

#214373

Purpose

Tet-OsTIR1(F74G) (AAVS1 insertion donor)

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

Tet-OsTIR1(F74G)

Expression

Mammalian

Promoter

Tet ON 3G promoter

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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