166,034 results

1. pNos-PE2-attB

   Plasmid

   #149549

   Purpose

   Expression of PE2 enzyme under control of the germ cell specific nanos promoter. Can be used to generate transgenic flies with vermillion+ selection.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   PE2
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   nanos (nos)

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-CMV-GCaMP6m

   Plasmid

   #40754

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Insert

   GCaMP6m
   

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-CMV-jGCaMP7f

   Plasmid

   #104483

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Insert

   jGCaMP7f
   

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-PD-1 (Y223F, Y248F)-mGFP

   Plasmid

   #180790

   Purpose

   Expression of human PD-1 (Y223F, Y248F) fused to mEGFP

   Depositor

   Enfu Hui

   Article

   Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   PD-1 (Y223F, Y248F) (PDCD1 Human)
   

   Use

   Lentiviral

   Tags

   mEGFP

   Mutation

   PD-1 (Y223F, Y248F)

   Promoter

   SFFV

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFD152

   Plasmid

   #125546

   Purpose

   derivative of pFD116 with an optimized RBS for E. coli

   Depositor

   David Bikard

   Article

   Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

   Insert

   optimized RBS
   

   Use

   CRISPR

   Promoter

   anhydrotetracycline Ptet promoter

   Available Since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKD239-1g-2

   Plasmid

   #90959

   Purpose

   Expresses S. baltica TtrR and mCherry constitutively and sfGFP under the PttrB185-269 promoter

   Depositor

   Jeffrey Tabor

   Article

   Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

   Inserts

   Tetrathionate response regulator
   Superfolder GFP
   mCherry
   

   Expression

   Bacterial

   Promoter

   Bba_J23100, Bba_J23109, and PttrB185-269

   Available Since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. CROPseq-multi-v2-Puro-BsmBI_entry (pRW1484)

   Plasmid

   #225752

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQ79

   Plasmid

   #175064

   Purpose

   expresses mutant of the huntingtin exon1 in mammalian cells

   Depositor

   Arnold Boersma

   Article

   Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

   Insert

   polyQ79 (HTT Human)
   

   Expression

   Mammalian

   Mutation

   mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

   Promoter

   CMV Promoter

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMK287 (mAID-Hygro)

   Plasmid

   #72825

   Purpose

   C-terminal tagging with mAID using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-Hygro
   

   Use

   CRISPR

   Tags

   mAID

   Expression

   Mammalian

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMF1706

    Plasmid

    #125582

    Purpose

    Expresses peroxisomal roGFP2 (po-roGFP2) in mammalian cells

    Depositor

    Marc Fransen

    Article

    Ivashchenko et al Mol Biol Cell. 2011 May;22(9):1440-51. d

    Insert

    po-roGFP2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-iGlucoSnFR-TS

    Plasmid

    #127952

    Purpose

    Expresses the iGlucoSnFR-TS biosensor for intracellular [glucose], with a fluorescence-lifetime readout, in mammalian cells

    Depositor

    Gary Yellen

    Article

    Diaz-Garcia et al J Neurosci Res. 2019 May 20. doi: 10.100

    Insert

    iGlucoSnFR-TS
    

    Use

    AAV

    Tags

    His6 and Xpress epitope

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. SIN40C.SFFV.Luciferase.IRES.GFP

    Plasmid

    #169308

    Purpose

    Constitutive overexpression of Luciferase cDNA with GFP as a reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Promoter

    SFFV

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. gRNA NIA TLS2

    Plasmid

    #196981

    Purpose

    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS2 mobility signal that confers graft mobility.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AtNIA1 gRNAs fused to TLS2 tags
    

    Expression

    Plant

    Promoter

    pU6-26, pU6-29

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR_TRE3G_EGFP_WPRE

    Plasmid

    #231954

    Purpose

    Lentiviral vector for inducible expression of the green fluorescent protein EGFP (TRE3G promoter)

    Depositor

    Leonardo Morsut

    Insert

    EGFP
    

    Use

    Lentiviral

    Available Since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA6A-EGFR WT

    Plasmid

    #42665

    Depositor

    Mien-Chie Hung

    Article

    Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

    Insert

    EGFR (EGFR Human)
    

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-eGFP-uTEV3

    Plasmid

    #234320

    Purpose

    Mammalian expression of uTEV3 tagged with an eGFP marker

    Depositor

    Mateo Sánchez

    Article

    Silva-Rojas et al Nat Biomed Eng. 2025 Jun 5. doi: 10.1038

    Insert

    eGFP-uTEV3
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Mutations respective to the wild type TEV: S219V …

    Promoter

    CMV

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmCherry-C1 mCherry-NLS

    Plasmid

    #58476

    Purpose

    Expresses a nuclear-targeted mCherry control construct, on a CMV reporter

    Depositor

    Dyche Mullins

    Article

    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

    Insert

    3X NLS with 3X stop codons
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-iCreV

    Plasmid

    #140135

    Purpose

    Can be used to generate AAV virus that will express light-inducible site-specific iCreV recombinase

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Has Service

    AAV PHP.eB and AAV1

    Insert

    iCreV
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-OptoTGFBRs

    Plasmid

    #118942

    Purpose

    Mammalian expression plasmid of OptoTGFBRs (optogenetically-activated TGFb receptors)

    Depositor

    Won Do Heo

    Article

    Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

    Insert

    OptoTGFBRs (Tgfb1, Tgfbr2 Mustard Weed, Mouse)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST-CMV-3xFLAG-gateway-EGFP

    Plasmid

    #122845

    Purpose

    N-terminal 3xFLAG tag and C-terminal EGFP tag. Gateway destination vector for mammalian expression.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3xFLAG and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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