173,019 results

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p15aC-GFP

Plasmid

#107862

Purpose

Control plasmid expressing GFP

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Insert

GFP

Tags

myc

Expression

Bacterial

Promoter

proD

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pQE80L-IL2 C125S

Plasmid

#104348

Purpose

Expresses human IL-2 (C125S)

Depositor

Alexander McLellan

Insert

Human IL-2 (C125S) (IL2 Human)

Tags

MRGSHHHHHGS-tag

Expression

Bacterial

Mutation

C125S mutation (TGT -> TCC)

Promoter

T5

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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BASU RaPID plasmid

Plasmid

#107250

Purpose

Expresses the BASU RaPID labeling protein

Depositor

Paul Khavari

Article

Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

Insert

BASU

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-puro-CMV-VC3AI

Plasmid

#78907

Purpose

Indicator of caspase-3-like protease activity

Depositor

Binghui Li

Article

Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

Insert

VC3AI

Use

Lentiviral

Tags

HA and Myc

Mutation

S320A, deletion of AA1-200

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pTS395_PhCMV-SB100

Plasmid

#169633

Purpose

Expression vector encoding PhCMV driven SB100 sleeping beauty transposase (PhCMV-SB100-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven SB100

Expression

Mammalian

Promoter

PhCMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pFB2C

Plasmid

#195285

Purpose

Bicistronic HSV-TK and Hygromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A Hygro

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0002

Plasmid

#179835

Purpose

Binary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoter

Depositor

Xiaohan Yang

Article

Yuan et al Hortic Res. 2021 Nov 1;8(1):234. doi: 10

Type

Empty backbone

Use

Binary vector for plant transformation

Expression

Plant

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pG106K

Plasmid

#191377

Purpose

Bacteroides - Escherichia coli shuttle vector with ErmF and Kan selection marker

Depositor

Janina Lewis

Article

Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

Type

Empty backbone

Use

Pg106

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pHBS1503 [IBB-GFP-mCherry3E]-[BFP-TDP43 WT]

Plasmid

#133327

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

None

Available Since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pSB-CMV-MCS-Puro dr_cPLA2-mKate2

Plasmid

#163844

Purpose

Used as a driver for the readout of cPLA2 activation in live cells with an mKate2 tag.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Insert

cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Tags

mKate2

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pWPI-TBX2

Plasmid

#185526

Purpose

Constitutive overexpression of human TBX2

Depositor

Sean Palecek, Eric Shusta

Article

Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

Insert

TBX2 (TBX2 Human)

Use

Lentiviral

Promoter

EF-1a

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pInt_attP1_LCS_kanR

Plasmid

#205300

Purpose

Integrating plasmid for ORBIT in E. coli. Contains library cloning site (LCS), which has transcriptional terminators to insulate reporter constructs.

Depositor

Scott Saunders

Article

Saunders et al bioRxiv 2023

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pREP-8xARE-GFP-SV40-BFP

Plasmid

#134910

Purpose

NRF2 reporter plasmid with GFP under control of 8 ARE elements and constitutively transcribed BFP

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Inserts

8xARE- minimal promoter - GFP
TagBFP

Expression

Mammalian

Promoter

8xARE - minimal promoter and SV40

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE EF1α dCas9-KRAB

Plasmid

#50919

Purpose

Constitutive dCas9-KRAB 2nd generation lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and KRAB

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1alpha

Available Since

March 28, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-MARCer-Blast

Plasmid

#160550

Purpose

labeling hypoxic cells

Depositor

Marc Vooijs

Article

Vermeer et al Dis Model Mech. 2020 Jul 30;13(7). pii:

Insert

MARCer (HIF1A Human)

Use

Lentiviral

Tags

eGFP-Cre

Promoter

CMV

Available Since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pmEmerald-ERGIC-53

Plasmid

#170717

Purpose

Expresses mEmerald-tagged human ERGIC-53 at high level in mammalian cells.

Depositor

Ke Xu

Article

Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

Insert

ERGIC-53 (LMAN1 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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PEmax_AAVS1_knockin_HDR_donor

Plasmid

#223000

Purpose

HDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PEmax

Use

Hdr donor

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

EF1α

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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PGK mScarlet-LC3B

Plasmid

#200083

Purpose

Mammalian expression of fluorescently-tagged marker for autophagic vesicles

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

Map1lc3b (Map1lc3b Rat)

Tags

mScarlet

Expression

Mammalian

Promoter

PGK

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-ChR2-WPREpA

Plasmid

#84471

Purpose

pAAV-RAM-ChR2

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Has Service

AAV8

Insert

Channelrhodopsin 2

Use

AAV

Tags

EYFP

Mutation

H134R

Promoter

TRE

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pET21-His6dNLP

Plasmid

#105498

Purpose

Expression of N-terminally 6His tagged Drosophila nucleoplasmin-like protein

Depositor

James Kadonaga

Article

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

His6dNLP

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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