161,641 results

1. pET51b-His-TEV-SNAP-tag_fast

   Plasmid

   #167271

   Purpose

   Overexpression of the fast variant (E30R) of SNAP-tag in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   SNAPf-tag
   

   Use

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   E30R

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLJC5-LAMP1-RFP-3xHA

   Plasmid

   #102932

   Purpose

   Express a lysosomal Tag

   Depositor

   David Sabatini

   Article

   Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

   Insert

   LAMP1
   

   Use

   Lentiviral

   Tags

   RFP-HA

   Expression

   Mutation

   Promoter

   ubc

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. BPK3079

   Plasmid

   #78741

   Purpose

   Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   AsCpf1 crRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCROPseq-VEX-optimized-scaffold

   Plasmid

   #203321

   Purpose

   Modified CROPseq vector for efficient CRISPR screens in hematopoiesis

   Depositor

   Vijay Sankaran

   Article

   Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDAS12069_U6-pegRNA-mCherry

   Plasmid

   #177180

   Purpose

   pegRNA cloning plasmid with mCherry marker

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-6xGLY-Strep-tagII-kanMX6

   Plasmid

   #20757

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti CMVtight eGFP Neo (w784-1)

   Plasmid

   #26586

   Purpose

   2nd generation lentiviral vector

   Depositor

   Eric Campeau

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Lentiviral; Tet-on advanced vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiCROP-PuroR-P2A-GFP-U6

   Plasmid

   #200029

   Purpose

   Lentiviral CROP-seq vector backbone with PuroR-P2A-GFP for cloning DNA Ticker Tapes and U6 for pegRNAs

   Depositor

   Jay Shendure

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   PuroR-P2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. MGAT-SEP-mRuby3

   Plasmid

   #200937

   Purpose

   cis/medial golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

   Depositor

   Nir Hacohen

   Article

   Liu et al Science. 2023 Aug 4;381(6657):508-514. d

   Inserts

   MGAT2 (MGAT2 Human)
   SEP-mRuby3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGMC00025 (aka pCW-dCas9-Blast)

    Plasmid

    #195316

    Purpose

    Dox inducible lentiviral dead Cas9

    Depositor

    Raj Chari

    Insert

    dead Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    D10A; H840A

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-EIN-FTO(32-505)

    Plasmid

    #186466

    Purpose

    Expresses human RNA demythelase protein FTO 32-505 amino acid sequence with N-terminal fusion with the N-domain of EI ( e.coli protein)

    Depositor

    Vincenzo Venditti

    Article

    Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055

    Insert

    Fat mass obesity associated protein (FTO Human)
    

    Use

    Tags

    6 Histidine-EIN (N-terminal domain of E.coli Enzy…

    Expression

    Bacterial

    Mutation

    amino acid 32-505

    Promoter

    T7

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. B-GRAM-H

    Plasmid

    #211706

    Purpose

    Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187L mutation (B-GRAM-H) in mammalian cells

    Depositor

    Yasunori Saheki

    Article

    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

    Insert

    B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    

    Use

    Tags

    ddFP-B3

    Expression

    Mammalian

    Mutation

    Changed Glycine 187 to Leucine

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. psiCheck2-SV40p-3xFLAG-HSVTKp-mCherry

    Plasmid

    #182377

    Purpose

    Dual expression construct encoding 3xFLAG and mCherry from separate promoters

    Depositor

    Chunghun Lim

    Article

    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

    Inserts

    3xFlag
    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    HSV TK promoter and SV40 promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-CMV-6xHis-SUMO1

    Plasmid

    #164939

    Purpose

    Expresses 6xHis tagged human Small Ubiquitin Like Modifier 1 (SUMO1) wild type cDNA

    Depositor

    Ji Luo

    Article

    Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Small Ubiquitin Like Modifier 1 (SUMO1 Human)
    

    Use

    Lentiviral

    Tags

    6XHIS

    Expression

    Mutation

    Promoter

    CMV

    Available since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMP71-tCD19-mGFP

    Plasmid

    #174609

    Purpose

    expresses membrane-associated (palmitoylated) EGFP and extracellular and transmembrane domains of murine CD19

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    CD19 extracellular and transmembrane domain (Cd19 Mouse)
    

    Use

    Tags

    palmitoylated EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. PGK-H2BmCherry

    Plasmid

    #21217

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    Phosphoglycerate kinase promoter (PGK1 Human)
    

    Use

    Lentiviral

    Tags

    H2B-mCherry

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRvCAHS3-CAHS3-mEGFP

    Plasmid

    #205006

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the RvCAHS3-mEGFP gene positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    RvCAHS3-mEGFP with 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
    

    Use

    Tardigrade expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. VN-alpha Synuclein

    Plasmid

    #89470

    Purpose

    expresses human alpha Synuclein fused to the N-terminal part of Venus

    Depositor

    Bradley Hyman, Tiago Outeiro

    Article

    Outeiro et al PLoS One. 2008 Apr 2;3(4):e1867. doi: 10

    Insert

    SNCA (SNCA Human)
    

    Use

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. EasyFusion T2A-H2B-TagBFP

    Plasmid

    #113086

    Purpose

    General donor vector with T2A-H2B-TagBFP coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    T2A-H2B-TagBFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRvTUB-mEGFP-NLS

    Plasmid

    #205011

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the tubulin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP-NLS flanked by 1kbp upstream and downstream of the tubulin gene from R. varieornatus
    

    Use

    Tardigrade expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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