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179,838 results

Showing: 6641 - 6660 of 179838 results

  1. LLP252 pEF1a-NLS-scFvGCN4-DNMT3a

    Plasmid
    #100941
    Purpose
    Single chain variable fragment antibody GCN4 fused to DNMT3a, binds SunTag, with 3xTy1 tag
    Depositor
    Ryan Lister
    Publication
    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.
    Insert
    scFvGCN4, sfGFP, GB1, DNMT3a catalytic domain (DNMT3A Synthetic, Human)
    Tags
    1xHA and 3xTy1
    Expression
    Mammalian
    Mutation
    D10A, H840A for dCas9
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100941-plasmid-map-sequence-id-206116

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  6. AAVS1-idCas9-vpr

    Plasmid
    #89985
    Purpose
    inducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locus
    Depositor
    Jie Na
    Publication
    Guo et al Protein Cell. 2017 Jan 23. doi: 10.1007/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-VPR
    Expression
    Mammalian
    Promoter
    TRE-tight
    Available since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89985-plasmid-map-sequence-id-180638

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  14. pSynSRE-Mut-T-Luc

    Plasmid
    #60490
    Purpose
    Contains a mutant version of the WT pSynSRE-T-Luc reporter plasmid. Four point mutations in the promoter SRE elements abolish SREBP-induced luciferase expression as described by Smith et al., 1988
    Depositor
    Timothy Osborne
    Publication
    Smith et al J Biol Chem. 1988 Dec 5;263(34):18480-7.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HMG-CoA synthase promoter (-324/-225) fused to HMG-CoA synthase TATAA (-28/+39) transcription initiator sequence
    Use
    Luciferase
    Tags
    Luciferase reporter
    Expression
    Mammalian
    Mutation
    Four point mutations (G->C or A->C) in the …
    Promoter
    Partial HMG-CoA synthase promoter (-324/-225) fus…
    Available since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60490-plasmid-map-sequence-id-332535

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  19. NLS-dCas9-trCIB1

    Plasmid
    #64119
    Purpose
    Photoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1
    Depositor
    Moritoshi Sato
    Publication
    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-trCIB1 fusion
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    dCas9 (D10A H840A, catalytically inactive), trCIB…
    Promoter
    CMV
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64119-plasmid-map-sequence-id-140078

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  20. pSBbi-RB

    Plasmid
    #60522
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and blasticidin resistance gene
    Depositor
    Eric Kowarz
    Publication
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60522-plasmid-map-sequence-id-97524

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Showing: 6641 - 6660 of 179838 results