165,744 results

1. pHH0103_PDCD6_EF-hand

   Plasmid

   #110009

   Purpose

   Protein expression and purification of PDCD6_EF-hand

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   PDCD6_EF-hand (PDCD6 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-PGK- dCas9-SunTag-P2A-HygR

   Plasmid

   #193137

   Purpose

   Expresses dCas9 fused to the SunTag scaffold

   Depositor

   Brad Rosenberg

   Article

   Danziger et al PLoS Pathog. 2022 Apr 14;18(4):e1010464.

   Insert

   dCas9-SunTag-T2A-HygR
   

   Use

   Lentiviral

   Promoter

   PGK

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAHYB-GFP

   Plasmid

   #85074

   Purpose

   Expression of GFP in Pichia pastoris

   Depositor

   Sheng Yang

   Article

   Yang et al Yeast. 2014 Apr;31(4):115-25.

   Insert

   GFP
   

   Expression

   Yeast

   Promoter

   AOX1

   Available Since

   March 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPonA-BI-Gl NORM-LacZA TER- LacZB

   Plasmid

   #86212

   Purpose

   beta Globin NMD construct with and without PTC under Bi-directional Ponasterone A inducible promoter

   Depositor

   Robert Singer

   Article

   Trcek et al Genes Dev. 2013 Mar 1;27(5):541-51. doi:

   Insert

   beta-Globin (HBB, Hbb Mouse, Human)
   

   Use

   Ponasterone a inducible promoter

   Tags

   lacZA or lacZB

   Expression

   Mammalian

   Mutation

   39TER in beta Globin in MCS II

   Promoter

   Ponasterone A inducible

   Available Since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTK168_mCherry-H2B

   Plasmid

   #46363

   Purpose

   visualizes chromosomes

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

   Insert

   H2B (H2BC11 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   D26G, V119I and S125D

   Promoter

   pBABE

   Available Since

   Aug. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCfB2337

   Plasmid

   #67555

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Expression

   Yeast

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAGM65881

   Plasmid

   #153215

   Purpose

   Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3XMEF2-luc

   Plasmid

   #32967

   Purpose

   Reporter gene for MEF2 activity with 3 MEF2 sites upstream of a c-fos minimal promoter

   Depositor

   Ron Prywes

   Insert

   MEF2 binding sites (MEF2A Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Promoter

   MEF2

   Available Since

   Feb. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. GPR55-DuET

   Plasmid

   #213285

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR55 (GPR55 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKM496 (deletions) Zeo

    Plasmid

    #109301

    Purpose

    ORBIT integrating plasmid for deletion of target gene, using zeocin as a selection marker.

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    Bxb1 attB site
    

    Use

    Orbit integrating plasmid

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-ING2

    Plasmid

    #13294

    Depositor

    Curtis Harris

    Article

    Pedeux et al Mol Cell Biol. 2005 Aug . 25(15):6639-48

    Insert

    ING2 (INGX Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

12. LT3REV

    Plasmid

    #111174

    Purpose

    Tet-regulated miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    DsRed and Venus

    Promoter

    T3G

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX302 ID2-V5 puro

    Plasmid

    #83096

    Purpose

    Lentiviral vector for constitutive expression of human ID2 with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    ID2 (ID2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

14. pFB2

    Plasmid

    #195282

    Purpose

    Bicistronic DTR and Puromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    DTR 2A Puro (HBEGF Human, Synthetic)
    

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. TRMT10C (RG9MTD1A-c113)

    Plasmid

    #110248

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    RG9MTD1 (TRMT10C Human)
    

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Promoter

    T7 - lacO

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUDP002

    Plasmid

    #103872

    Purpose

    Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Inserts

    hph
    Spcas9 D147Y P411T
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Yeast

    Mutation

    D147Y P411T

    Promoter

    Arxula adeninivorans TEF1 and Ashbya gossypii (Er…

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Clathrin-mhYFP

    Plasmid

    #186527

    Purpose

    Localization of monomeric hyperfolder YFP fluorescent protein to clathrin

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Monomeric hyperfolder YFP
    

    Tags

    Clathrin

    Expression

    Mammalian

    Mutation

    hfYFP-S147P/L195M/V206K

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. R777-E185 Hs.PLXNB1

    Plasmid

    #70469

    Purpose

    Gateway ORF clone of human PLXNB1 [NM_001130082.2] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PLXNB1 (PLXNB1 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGIP

    Plasmid

    #26777

    Purpose

    Lentiviral vector for stable integration of GFP expression cassette with puromycin selection.

    Depositor

    Linzhao Cheng

    Article

    Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

    Insert

    EF1a-GFP-IRES-Puro (gfp )
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. N1-G-Ca-FLITS

    Plasmid

    #191465

    Purpose

    Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    G-Ca-FLITS
    

    Expression

    Mammalian

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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