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Enhanced Repressor Domain for TAL Effector (SID4X)
(Plasmid #43882)

Full plasmid sequence is not available for this item.



Item Catalog # Description Quantity Price (USD)
Plasmid 43882 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2710

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    SID4X Domain
  • Alt name
    SID domain
  • Alt name
    Enhanced Repressor Domain for TAL Effector (SID4X)
  • Alt name
    mSin Interaction Domain
  • Insert Size (bp)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XbaI (destroyed during cloning)
  • 5′ sequencing primer pUC57 Forward (GTAAAACGACGGCCAGTG)
  • 3′ sequencing primer pUC57 Reverse (GGAAACAGCTATGACCATG)
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This enhanced SID domain (SID4X domain) is to be used with TALE effectors to create TALE repressors, as described in Cong et al. Nature Communications. This new domain replaces the previous SID domain reported in the original article.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Enhanced Repressor Domain for TAL Effector (SID4X) was a gift from Feng Zhang (Addgene plasmid # 43882 ; ; RRID:Addgene_43882)
  • For your References section:

    Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Cong L, Zhou R, Kuo YC, Cunniff M, Zhang F. Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. 10.1038/ncomms1962 PubMed 22828628