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pAAV_TALE-TF(VP64)-BB_V3
(Plasmid #42581)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 42581 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV
  • Total vector size (bp) 6119
  • Vector type
    Mammalian Expression, AAV ; Adeno-associated Virus ()

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    Backbone contains ccdb gene, serve as a negative selection cassette. Please use ccdb resistant strain for maintenance of the plasmid.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TALE-TF(VP64)-BB
  • Alt name
    TALE Backbone
  • Alt name
    TALE VP64
  • Insert Size (bp)
    2015
  • Promoter CAG hybrid
  • Tag / Fusion Protein
    • 3XFlag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI/BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CAG-forward
  • 3′ sequencing primer BGH-reverse
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This is a new collection of constructs described in Cong et al. Nature Communications and also an updated TALE Tool Box containing Adeno-associated Virus (AAV) TALE Backbone constructs that allow packaging of TAL transcription factors (TALE-TF) and TALE nucleases (TALEN) into AAV. All constructs are from Feng Zhang lab at Broad Institute/MIT.

IMPORTANT: please note when using the new AAV TALE Backbones, the last 0.5 (half) repeat is not encoded in the backbone vector but instead need to be supplemented as a separate piece. Please refer to our website at http://taleffector.genome-engineering.org/ for more information on these new backbone vectors in the 'Protocols - Reagents' section under 'Special Note for New Adeno-associated Virus (AAV) TALE-TF and TALEN Backbones'.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV_TALE-TF(VP64)-BB_V3 was a gift from Feng Zhang (Addgene plasmid # 42581 ; http://n2t.net/addgene:42581 ; RRID:Addgene_42581)
  • For your References section:

    Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Cong L, Zhou R, Kuo YC, Cunniff M, Zhang F. Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. 10.1038/ncomms1962 PubMed 22828628