|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35681||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
MutationT18A and S19A
Entrez GeneMYL9 (a.k.a. LC20, MLC-2C, MLC2, MRLC1, MYRL2)
- Promoter CMV
/ Fusion Protein
- EGFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site AgeI (not destroyed)
- 5′ sequencing primer CMV fwd
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
This plasmid is identical to the one published in Beach et al., 2011, except the tet-inducible promoter has been replaced with a constitutive CMV promoter.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEGFP-MRLC T18A,S19A was a gift from Tom Egelhoff (Addgene plasmid # 35681 ; http://n2t.net/addgene:35681 ; RRID:Addgene_35681)
For your References section:Analysis of the role of Ser1/Ser2/Thr9 phosphorylation on myosin II assembly and function in live cells. Beach JR, Licate LS, Crish JF, Egelhoff TT. BMC Cell Biol. 2011 Dec 2;12:52. 10.1186/1471-2121-12-52 PubMed 22136066
Map uploaded by the depositor.