159,117 results

1. M30: pHR-SFFVp- HNRNPA1C::mCherry::SspB

   Plasmid

   #122668

   Purpose

   C terminal domain of hnRNPA1 (186-320) tagged with SspB and mCherry.

   Depositor

   Clifford Brangwynne

   Article

   Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

   Insert

   HNRNPA1 (HNRNPA1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry-SspB

   Expression

   Mammalian

   Mutation

   Contains only amino acids 186-230

   Promoter

   SFFV

   Available since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PS-CFP2-TOMM20-N-10

   Plasmid

   #57250

   Purpose

   Localization: Outer Mitochondrial Membrane, Excitation: 400 / 490, Emission: 468 / 511

   Depositor

   Michael Davidson

   Insert

   TOMM20 (TOMM20 Human)
   

   Use

   Tags

   PS-CFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 13, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pG1K

   Plasmid

   #71741

   Purpose

   Modular shuttle vector for Geobacillus and E. coli

   Depositor

   Tom Ellis

   Article

   Reeve et al ACS Synth Biol. 2016 Jul 1.

   Type

   Empty backbone

   Use

   Synthetic Biology; Shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMVHA SUZ12

   Plasmid

   #24232

   Purpose

   Mammalian expression of human SUZ12 with HA tag

   Depositor

   Kristian Helin

   Article

   Pasini et al EMBO J. 2004 Oct 13. 23(20):4061-71.

   Insert

   SUZ12 (SUZ12 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. M13KO7-ΔM13-ΔgeneIII-ΔgeneVI

   Plasmid

   #80840

   Purpose

   Modified helper phage

   Depositor

   Mark Isalan

   Article

   Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

   Inserts

   M13 gene II
   M13 gene X
   M13 gene V
   M13 gene VII
   M13 gene IX
   M13 gene VIII
   M13 gene I
   M13 gene XI
   M13 gene IV
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD-mWatermelon

   Plasmid

   #190625

   Purpose

   Bacterial expression of mWatermelon GFP

   Depositor

   Robert Campbell

   Article

   Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

   Insert

   mWatermelon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-GFP1-10

   Plasmid

   #78591

   Purpose

   Express mCherry-GFP1-10 in mammalian cells

   Depositor

   Yihong Ye

   Article

   Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

   Insert

   GFP 1-10
   

   Use

   Tags

   mCherry2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-CAG-hCas3

   Plasmid

   #134920

   Purpose

   Components for genome editing in mammalian cells with pCAG-All-in-one-hCascade and pBS-U6-crRNA-targeted.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   Cas3 with bpNLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. CCR5-DuET

   Plasmid

   #213201

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CCR5 (CCR5 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-Sport6-CD63-pHluorin

    Plasmid

    #130901

    Purpose

    Expression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHluorin (CD63 Human, Aequorea victoria)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    pHluorin inserted between Gln-36 and Leu-37 in ex…

    Promoter

    CMV

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Rat NCX1.1 (pcDNA3.1+)

    Plasmid

    #75190

    Purpose

    Mammalian expression of SLC8A1

    Depositor

    Jonathan Lytton

    Article

    Lee et al J Biol Chem. 1994 May 27;269(21):14849-5

    Insert

    SLC8A1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. DR274

    Plasmid

    #42250

    Purpose

    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR; Zebrafish expression

    Tags

    Expression

    Worm

    Mutation

    Promoter

    T7

    Available since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. PCMV4-3 HA/IkB-alpha(SS32,36AA)

    Plasmid

    #24143

    Depositor

    Warner Greene

    Article

    Sun et al Mol Cell Biol. 1996 Mar . 16(3):1058-65.

    Insert

    IkB alpha (NFKBIA Human)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    S32A S36A

    Promoter

    Available since

    March 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)

    Plasmid

    #140005

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. MIP 227 CoMiP 4in1 wo shRNA p53

    Plasmid

    #63727

    Purpose

    To express Oct4, Klf4, Sox2, and c-Myc. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).

    Depositor

    Joseph Wu, Mark Kay

    Article

    Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103

    Insert

    Mini-intronic-plasmid intron (POU5F1, KLF4, SOX2, MYC Human)
    

    Use

    Tags

    IRES-dtomato

    Expression

    Mammalian

    Mutation

    codon-optimised

    Promoter

    SFFV

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXs-c-Myc

    Plasmid

    #13375

    Purpose

    Retroviral expression of mouse c-Myc

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    myelocytomatosis oncogene (Myc Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEX-A-U6-gRNA

    Plasmid

    #65626

    Purpose

    Empty vector for the expression U6 driven gRNA

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    U6-gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCh-Sec61 beta

    Plasmid

    #49155

    Purpose

    expression of Sec61 beta fused to mCherry, used as a general ER marker

    Depositor

    Gia Voeltz

    Article

    Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

    Insert

    Sec61 beta (SEC61B Human)
    

    Use

    Tags

    mCherry*

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTXB1.Tat(48-57)-Cre recombinase

    Plasmid

    #165042

    Purpose

    Expression of Tat(48-57) conjugated Cre recombinase for protein purification (Intein-chitin binding domain fussion)

    Depositor

    Dokyun Na

    Article

    Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

    Insert

    Cre recombinase
    

    Use

    Tags

    Mxe Intein-Chitin binding domain and Tat(48-57)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET23b-15F11-HA-mEGFP-6xHis

    Plasmid

    #129593

    Purpose

    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    

    Use

    Tags

    6xHis-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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