165,744 results

1. pCMV-PTEN

   Plasmid

   #28298

   Depositor

   Maria-Magdalena Georgescu

   Article

   Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

   Insert

   PTEN (PTEN Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Sept. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. TET-pLKO.1 PURO shID2 #1

   Plasmid

   #83086

   Purpose

   Lentiviral shRNA vector for inducible knockdown of human ID2

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   shID2 (ID2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

3. MAC_N_CDK8

   Plasmid

   #111685

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   CDK8 (CDK8 Human)
   

   Expression

   Mammalian

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. FASN

   Plasmid

   #38793

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   FASN (FASN Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

5. T7-3e5

   Plasmid

   #106990

   Purpose

   T7 transcription template for 3e5

   Depositor

   Melissa Moore

   Article

   Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

   Insert

   3e5
   

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag‐mTim3

   Plasmid

   #49208

   Purpose

   expresses Flag tagged mouse Tim3

   Depositor

   Lawrence Kane

   Article

   Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

   Insert

   Tim3 (Havcr2 Mouse)
   

   Tags

   FLAG and signal sequence

   Expression

   Mammalian

   Mutation

   no Tim3 signal sequence

   Promoter

   EF1a

   Available Since

   Dec. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCfB2224

   Plasmid

   #67547

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XI-2 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Expression

   Yeast

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX304 CMV OMM-FKBP-V5-sTurboID (N)

   Plasmid

   #153006

   Purpose

   Expresses split-TurboID N-terminal fragment targeted to outer mitochondrial membrane

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   OMM (Tom20)-sTurboID (N) (L73)
   

   Use

   Lentiviral

   Tags

   FKBP and V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCardinal-Vimentin-7

   Plasmid

   #56178

   Purpose

   Localization: Intermediate Filaments, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   Vimentin (VIM Human)
   

   Tags

   mCardinal

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pHN922

    Plasmid

    #178975

    Purpose

    Expression of GST-His8-GFP-nanobody

    Depositor

    Kazuhiro Abe

    Insert

    GST-His8-GFP-nanobody
    

    Use

    Affinity Reagent/ Antibody

    Tags

    GST, His8

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCfB2194

    Plasmid

    #67545

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae X-4 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Expression

    Yeast

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TRUPATH Triple Ga15

    Plasmid

    #196060

    Purpose

    Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlpha15-RLuc8 (GNA15 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. CROP_C9_Puro_HDAC3

    Plasmid

    #183299

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets HDAC3 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    HDAC3
    

    Use

    Lentiviral

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-TRAIL

    Plasmid

    #10953

    Depositor

    Bingliang Fang

    Article

    Kagawa et al Cancer Res. 2001 Apr 15. 61(8):3330-8.

    Insert

    Tumor necrosis factor alpha related apoptosis inducing ligand (TNFSF10 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBB572

    Plasmid

    #27397

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

    Insert

    ibpA, ibpB (ibpB, ibpA E.coli)
    

    Expression

    Bacterial

    Available Since

    March 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. psiCHECK2-miR-142-3p

    Plasmid

    #135716

    Purpose

    Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-142-3p)

    Depositor

    Florian Karreth

    Insert

    miR-142-3p reverse complement
    

    Expression

    Mammalian

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSEVAT7K1A

    Plasmid

    #58302

    Purpose

    GeneGuard vector cassette: thyA, ColE2 COR, Kid toxin; with ampR as cargo.

    Depositor

    Tom Ellis

    Article

    Wright et al ACS Synth Biol. 2014 May 13.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFAST-BAC-Cdc20wt (2193)

    Plasmid

    #39850

    Depositor

    Jonathon Pines

    Article

    Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

    Insert

    Cdc20 (CDC20 Human)
    

    Use

    Baculovirus expression

    Tags

    His

    Available Since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA5/FRT/TO(Neo)-Venus-Flag-WT wobbled cdc20 (1991)

    Plasmid

    #61848

    Purpose

    for making stable RPE cells expressing WT cdc20

    Depositor

    Jonathon Pines

    Insert

    cdc20 (CDC20 Human)
    

    Tags

    Flag and Venus

    Expression

    Mammalian

    Mutation

    wobbled

    Promoter

    CMV

    Available Since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pTF-ahpC-rfp

    Plasmid

    #153035

    Purpose

    For CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    CRISPR array
    

    Expression

    Bacterial

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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