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UbC-TRE-mSOD2-tTS-IRES-EGFP
(Plasmid #12390)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12390 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    UbC-TRE-tTS-IRES-EGFP
  • Backbone size w/o insert (bp) 7700
  • Vector type
    Mammalian Expression, RNAi
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mSOD2 shRNA
  • Alt name
    Sod2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    110
  • Entrez Gene
    Sod2 (a.k.a. MnSOD, Sod-2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See Additional Documents link above for cloning protocol, map, and annotated sequence from the author.

Note that there is a EcoRI and a KpnI site in the polylinker region. You will need to cut the plasmid with BamHI and religate to eliminate these sites before you could EcoRI and KpnI for cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UbC-TRE-mSOD2-tTS-IRES-EGFP was a gift from Zuoshang Xu (Addgene plasmid # 12390 ; http://n2t.net/addgene:12390 ; RRID:Addgene_12390)
  • For your References section:

    Multiple shRNAs expressed by an inducible pol II promoter can knock down the expression of multiple target genes. Xia XG, Zhou H, Xu Z. Biotechniques. 2006 Jul . 41(1):64-8. 10.2144/000112198 PubMed 16869515