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V406 4HA-CKIe(K38R)
(Plasmid #13725)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13725 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCEP4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 10200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    casein kinase 1 epsilon
  • Alt name
    CKIe
  • Alt name
    CK1e
  • Alt name
    casein kinase i epsilon
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Mutation
    kinase-inactive due to K38R mutation
  • GenBank ID
    L37043
  • Entrez Gene
    CSNK1E (a.k.a. CKIe, CKIepsilon, HCKIE)
  • Tag / Fusion Protein
    • 4 HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site SfiI (not destroyed)
  • 5′ sequencing primer pCEP forward
  • 3′ sequencing primer EBV Reverse
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    V406 4HA-CKIe(K38R) was a gift from David Virshup (Addgene plasmid # 13725 ; http://n2t.net/addgene:13725 ; RRID:Addgene_13725)
  • For your References section:

    Regulation of casein kinase I epsilon and casein kinase I delta by an in vivo futile phosphorylation cycle. Rivers A, Gietzen KF, Vielhaber E, Virshup DM. J Biol Chem. 1998 Jun 26. 273(26):15980-4. 10.1074/jbc.273.26.15980 PubMed 9632646