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V406 4HA-CKIe(K38R)
(Plasmid #13725)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13725 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 13725-D50 50 μg of DNA in Tris buffer $495
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DNA 13725-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCEP4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 10200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    casein kinase 1 epsilon
  • Alt name
    CKIe
  • Alt name
    CK1e
  • Alt name
    casein kinase i epsilon
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Mutation
    kinase-inactive due to K38R mutation
  • GenBank ID
    L37043
  • Entrez Gene
    CSNK1E (a.k.a. CKIe, CKIepsilon, HCKIE)
  • Tag / Fusion Protein
    • 4 HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site SfiI (not destroyed)
  • 5′ sequencing primer pCEP forward
  • 3′ sequencing primer EBV Reverse
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    V406 4HA-CKIe(K38R) was a gift from David Virshup (Addgene plasmid # 13725 ; http://n2t.net/addgene:13725 ; RRID:Addgene_13725)

  • For your References section:

    Regulation of casein kinase I epsilon and casein kinase I delta by an in vivo futile phosphorylation cycle. Rivers A, Gietzen KF, Vielhaber E, Virshup DM. J Biol Chem. 1998 Jun 26. 273(26):15980-4. 10.1074/jbc.273.26.15980 PubMed 9632646
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