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Rc/CMV cyclin D1 HA
(Plasmid #8948)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 8948 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5542
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    cyclin D1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    CCND1 (a.k.a. BCL1, D11S287E, PRAD1, U21B31)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A PCR product was digested with StuI and BspEI and the resulting ~250 bp fragment was used to replace the corresponding wildtype fragment. This resulted in the addition of the HA epitope after the final amino acid of cyclin D1 and reiteration of the final two cyclind D1 residues (DI) after the tag. In addition, 80 bp of the 3' UTS is deleted. For parent plasmid information, see Author's Map link for Rc/CMV cyclin D1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Rc/CMV cyclin D1 HA was a gift from Philip Hinds (Addgene plasmid # 8948 ; ; RRID:Addgene_8948)
  • For your References section:

    Multiple functions of D-type cyclins can antagonize pRb-mediated suppression of proliferation. Baker GL, Landis MW, Hinds PW. Cell Cycle. 2005 Feb . 4(2):330-8. 10.4161/cc.4.2.1485 PubMed 15684604