Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

161,641 results

Showing: 6741 - 6760 of 161641 results

  1. pHSN6A01

    Plasmid
    #50586
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-VP64
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Expression
    Mutation
    dCas9 = defective in DNA cleavage; 4×minimal VP16…
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50586-plasmid-map-sequence-id-258465

    Enlarge

  3. CYP2C19

    Plasmid
    #69606
    Purpose
    For protein expression of CYP2C19
    Depositor
    Joyce Goldstein
    Insert
    CYP2C19 (CYP2C19 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    deletion of AA1-8 and mutation I331V
    Promoter
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    69606-plasmid-map-sequence-id-193146

    Enlarge

  5. pCre-P2A-mCherry

    Plasmid
    #212105
    Purpose
    Expression of Cre and mCherry in mammalian cells
    Depositor
    Daekee Lee
    Insert
    Cre recombinase and mCherry
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    212105-plasmid-map-sequence-id-419563

    Enlarge

  10. pAK_lentiguide_DR30_Puro

    Plasmid
    #134839
    Purpose
    Lentiviral expression CasRx guideRNA
    Depositor
    Michael Yaffe
    Insert
    CasRx guideRNA backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    Nov. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    134839-plasmid-map-sequence-id-265965

    Enlarge

  14. Sniper2L

    Plasmid
    #193856
    Purpose
    Expresses human codon-optimized Sniper2L and blasticidin resistance: EFS promoter-Sniper2L-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/
    Insert
    Sniper2L
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    E1007L
    Promoter
    EFS
    Available since
    March 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    193856-plasmid-map-sequence-id-382455

    Enlarge

  15. pAK_DR30_EF1a_CasRx_Hygro

    Plasmid
    #134841
    Purpose
    Lentiviral all in one expression of CasRx and guideRNA
    Depositor
    Michael Yaffe
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Tags
    T2A Hygromycin
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    134841-plasmid-map-sequence-id-311015

    Enlarge

  18. NL4-3 mCherry Luciferase

    Plasmid
    #44965
    Purpose
    HIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.
    Depositor
    Warner Greene
    Article
    Lassen et al PLoS One. 2012;7(1):e30176. doi: 10.1371
    Insert
    mCherry, Luciferase, IRES
    Use
    Lentiviral
    Tags
    Luciferase, IRES and mCherry with D212G
    Expression
    Mutation
    S34C mutation in Nef
    Promoter
    Available since
    Oct. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    44965-plasmid-map-sequence-id-352462

    Enlarge

  19. TFORF3361

    Plasmid
    #144837
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MSC (MSC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    144837-plasmid-map-sequence-id-391555

    Enlarge

Showing: 6741 - 6760 of 161641 results