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pSNAPf-ADRβ2 Control Plasmid
(Plasmid #101123)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 101123 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSNAP
  • Backbone size w/o insert (bp) 5925
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418) ; Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Beta-2-Adrenergic Receptor
  • Alt name
    ADRβ2
  • Alt name
    ADRB2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1239
  • Entrez Gene
    ADRB2 (a.k.a. ADRB2R, ADRBR, B2AR, BAR, BETA2AR)
  • Promoter CMV
  • Tag / Fusion Protein
    • SNAP-tag (SNAPf) (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SbfI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer GACGCAAATGGGCGGTAG (CMV Forward Primer)
  • 3′ sequencing primer AGGGAGTACTCACCCCAACA (ST1 Reverse Primer)
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Originally cloned at New England Biolabs.The ADRBeta2 insert is a C-terminal insert. The signal peptide fused to the N-terminus of SNAPf is based on the 5HT3A serotonin receptor and is 72 nt.

This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at [email protected]. For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSNAPf-ADRβ2 Control Plasmid was a gift from New England Biolabs & Ana Egana (Addgene plasmid # 101123 ; http://n2t.net/addgene:101123 ; RRID:Addgene_101123)