New England Biolabs Cell-Imaging Plasmid Collection
New England Biolabs offers non-toxic protein labeling systems based on SNAP-, CLIP-, ACP-, and MCP-tags for visualization of proteins inside living or fixed cells. The plasmids associated with this technology are available at Addgene for distribution to the research community.
- Allows for visualization of multiple proteins simultaneously with dual labeling when used with two substrates
- Can be used in live or fixed cells, and for internal or surface proteins
- Single constructs are compatible with multiple applications (different fluorophores, biotin, bead purification, blocking agents)
- ACP- and MCP-tags are used exclusively for surface proteins and use enzymatic binding to attach substrate
- Control plasmids with a recognizable sub-cellular localization (membrane, nucleus, etc.) are also available
- Non-toxic to living cells
SNAP-tag is based on the human O6-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein, and is suitable for both cell-permeant and non-cell-permeant proteins.
CLIP-tag is identical in function to SNAP-tag but reacts with benzylcytosine rather than benzylguanine derivatives to allow for dual labeling and visualization of complementary protein function or localization.
ACP and MCP tags
ACP- and MCP-tags are small protein tags based on the acyl carrier protein. Substrates are derivatives of Coenzyme A (CoA). These tags are particularly well suited for labeling cell-surface proteins, and are useful for labeling secreted proteins with disulfide bridges such as antibodies. MCP-tag is a mutant of ACP-tag, and is labeled by SFP Synthase but not by ACP Synthase, thereby permitting selective simultaneous labeling in a sample. Please see the note below for information on substrates and synthases needed for use of these tags.
|101137||pSNAP-tag (T7) Vector||SNAP-tag||Empty backbone for bacterial expression under T7 control|
|101135||pSNAP-tag (m) Vector||SNAP-tag||Empty backbone for stable and transient mammalian expression|
|101133||pSNAP-CaaX Control Plasmid||SNAP-tag||Control for endomembrane (CaaX box) localization|
|101129||pSNAPf-Cox8A Control Plasmid||SNAP-tag||Control for mitochondrial localization|
|101124||pSNAPf-H2B Control Plasmid||SNAP-tag||Control for nuclear localization|
|101123||pSNAPf-ADRβ2 Control Plasmid||SNAP-tag||Control for cell surface localization|
|101136||pCLIP-tag (m) Vector||CLIP-tag||Empty backbone for stable and transient mammalian expression|
|101134||pCLIPf-H2B Control Plasmid||CLIP-tag||Control for nuclear localization|
|101130||pCLIPf-Cox8A Control Plasmid||CLIP-tag||Control for mitochondrial localization|
|101125||pCLIPf-NK1R Control Plasmid||CLIP-tag||Control for cell surface localization|
|101127||pMCP-tag (m) Vector||ACP/MCP-tag||Empty backbone for transient mammalian expression|
|101126||pACP-tag (m)- 2 Vector||ACP/MCP-tag||Empty backbone for stable and transient mammalian expression|
|101132||pMCP-GPI Control Plasmid||ACP/MCP-tag||Control for cell surface localization|
|101131||pACP-GPI Control Plasmid||ACP/MCP-tag||Control for cell surface localization|
|101128||pACP-ADRβ2 Control Plasmid||ACP/MCP-tag||Control for cell surface localization|
SNAP and CLIP tagsSNAP and CLIP tag FAQs (252.2 KB)
ACP and MCP tags
Please note: New England Biolabs (NEB) has recently discontinued the ACP-tag/MCP-tag product line from their product portfolio, including the CoA-derivative substrates and the ACP and SFP Synthases. Users may still generate their own fluorescent or non-fluorescent CoA substrates by purchasing coenzyme A disodium salt from a commercial supplier and conjugating it to the label of their choice according to the methods described by George et al. 2004. The ACP and SFP Synthases (AcpS and Sfp, respectively) may be expressed and purified as described previously in Stachelhaus et al. 1998
- George N, Pick H, Vogel H, Johnsson N, Johnsson K. 2004. Specific labeling of cell surface proteins with chemically diverse compounds. J Am Chem Soc. 126(29):8896-7. PMID:15264811
- Stachelhaus T, Mootz HD, Bergendahl V, Marahiel MA.1998. Peptide bond formation in nonribosomal peptide biosynthesis. Catalytic role of the condensation domain. J Biol Chem. 273(35):22773-81. PMID:9712910
- Vivero-Pol L, George N, Krumm H, Johnsson K, Johnsson N. 2005. Multicolor imaging of cell surface proteins. J Am Chem Soc. 127(37):12770-1. PMID:16159249