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LentiGuide-Puro-CTRLg2 (CG510)

Plasmid

#139456

Purpose

Lentiviral vector with non-targeting gRNA and puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pPSU20-40-60-80

Plasmid

#173839

Purpose

Coexpresses 20, 40, 60 and 80 kD Penn State ladder proteins

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABPACCBPv3

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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TK4_SH4-1_targeting

Plasmid

#254452

Purpose

TK4 targeting vector containing left and right homology arms for the SH4-1 locus

Depositor

Marius Wernig

Article

Uenaka et al Cell Stem Cell. 2026 Feb 13:S1934-5909(2

Inserts

mycNLS-mScarlet-T2A-mNeonGreen-PEST
puroR-T2A-mycNLS-mTagBFP2

Use

Piggybac

Tags

PEST, T2A-mycNLS-mTagBFP2, and c-myc-NLS

Expression

Mammalian

Promoter

CAG and EF1a

Available Since

April 24, 2026

Availability

Academic Institutions and Nonprofits only

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TK4_CLYBL_targeting

Plasmid

#254455

Purpose

TK4 targeting vector containing left and right homology arms for the CLYBL locus

Depositor

Marius Wernig

Article

Uenaka et al Cell Stem Cell. 2026 Feb 13:S1934-5909(2

Inserts

mycNLS-mScarlet-T2A-mNeonGreen-PEST
puroR-T2A-mycNLS-mTagBFP2

Use

Piggybac

Tags

PEST, T2A-mycNLS-mTagBFP2, and c-myc-NLS

Expression

Mammalian

Promoter

CAG and EF1a

Available Since

April 24, 2026

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVTv2_3xFLAG-SUMO_Eu1-HsZSWIM8-3xHA

Plasmid

#254347

Purpose

Expression of ZSWIM8 for protein purification

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human ZSWIM8 (KIAA0913 Human)

Tags

3xFLAG-SUMO(Eu1) and 3xHA

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pRK5_HsELOB

Plasmid

#254348

Purpose

Expression of ELOB for protein purification

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human Elongin B (ELOB Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pRK5_HsELOC

Plasmid

#254349

Purpose

Expression of ELOC for protein purification

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human Elongin C (ELOC Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pSL8215 (dCas12f–σE (sigmaE) system, entry vector)

Plasmid

#254272

Purpose

Encodes E. coli codon-optimized dCas12f and σE (sigmaE) from Flagellimonas taeanensis driven by constitutive promoters, with BsaI-containing restriction sites for guide RNA cloning

Depositor

Samuel H. Sternberg

Article

Hoffmann et al Nature. 2026 Mar 4. doi: 10.1038/s41586-

Inserts

dCas12f
sigma E

Use

CRISPR

Expression

Bacterial

Promoter

J23105

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pSL7458 (Fta RNA polymerase)

Plasmid

#254269

Purpose

Encodes E. coli codon-optimized rpoA, rpoB, rpoC, and rpoZ from Flagellimonas taeanensis driven by an inducible T7 promoter

Depositor

Samuel H. Sternberg

Article

Hoffmann et al Nature. 2026 Mar 4. doi: 10.1038/s41586-

Inserts

rpoA
rpoB
rpoC
rpoZ

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pHalo2X-actin

Plasmid

#251421

Purpose

Mammalian expression vector for expressing human beta-actin with an N-terminal HaloTag

Depositor

Thomas Friedman, Takushi Miyoshi

Article

Miyoshi et al Nat Commun. 2025 Sep 1;16(1):8149. doi:

Insert

beta-actin (ACTB Human)

Tags

HaloTag

Expression

Mammalian

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-dnGFP-OGT(13)

Plasmid

#247677

Purpose

Expresses full-length OGT fused to a destabilized GFP nanobody

Depositor

Christina Woo

Article

Mody et al Cell Chem Biol. 2025 Dec 18;32(12):1486-

Insert

HA-dnGFP-OGT(13) (OGT Human)

Tags

HA-tag

Expression

Mammalian

Promoter

T7

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-dnGFP-OGT(13, K852A)

Plasmid

#247678

Purpose

Expresses catalytically inactive full-length OGT K852A mutant fused to a destabilized GFP nanobody

Depositor

Christina Woo

Article

Mody et al Cell Chem Biol. 2025 Dec 18;32(12):1486-

Insert

HA-dnGFP-OGT(13, K852A) (OGT Human)

Tags

HA-tag

Expression

Mammalian

Mutation

K852A mutation made in OGT

Promoter

T7

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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(380-bp-TET-DIAL-YB-TATA)-mGL-bGH_pKG3262

Plasmid

#246361

Purpose

380-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of DOX and rtTA and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET-hisTdT

Plasmid

#216674

Purpose

Expresses his-tagged N-terminus truncated TdT in E. coli hosts

Depositor

Po-Min Chiang

Article

Hsueh et al PLoS Biol. 2025 Dec 15;23(12):e3003537.

Insert

terminal deoxynucleotidyl transferase

Tags

his-tag

Expression

Bacterial

Mutation

1-138 truncation

Promoter

T7

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_C-StrepIII

Plasmid

#231300

Purpose

For transiently expressing fusion proteins with C terminal StrepIII tags in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

StrepIII

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pSGEP-shFos_1

Plasmid

#239618

Purpose

shRNA-mediated knockdown of murine Fos

Depositor

Reza Kalhor

Article

Handler et al Cell Rep. 2025 Dec 15;44(12):116701. doi

Insert

shFos_1 (Fos Mouse)

Use

Lentiviral

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pSGEP-shFos_2

Plasmid

#239619

Purpose

shRNA-mediated knockdown of murine Fos

Depositor

Reza Kalhor

Article

Handler et al Cell Rep. 2025 Dec 15;44(12):116701. doi

Insert

shFos_2 (Fos Mouse)

Use

Lentiviral

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pJM220-PhoA-mNeongreen-J23101-mScarletI

Plasmid

#240201

Purpose

MiniTn7 donor plasmid for constructing a ratiometric reporter for P limitation in Pseudomonas synxantha. A strong Anderson promoter (J23101) drives expression of mScarlet-I

Depositor

Richard Murray

Article

Larsson et al ACS Synth Biol. 2024 Jan 19;13(1):384-39

Inserts

mNeongreen
mScarlet-I

Promoter

J23101 and PphoA

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT_CAG-ITPKA-mScarlet-I

Plasmid

#229778

Purpose

Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporter

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

mScarlet-I

Use

Synthetic Biology

Tags

ITPKA

Expression

Mammalian

Promoter

CAG

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(-7)-neg_tag1

Plasmid

#219419

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

GFP(-7)-neg_tag1

Tags

DEEEDD and MGHHHHHGG

Expression

Bacterial

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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