165,744 results

1. AAV-hSyn-ASAP4e-Kv-WPRE

   Plasmid

   #201031

   Purpose

   Expresses somatically enriched ASAP4e in neurons, can be used to package AAV

   Depositor

   Michael Lin

   Article

   Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

   Insert

   ASAP4e-Kv
   

   Use

   AAV

   Available Since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. araBAD-BCD20-ClpP-BCD17-ClpX

   Plasmid

   #153301

   Purpose

   Expresses clpX and clpP in E.Coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

   Inserts

   clpX (clpX Escherichia coli)
   clpP (clpP Escherichia coli)
   

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. SypHer

   Plasmid

   #48250

   Purpose

   pH sensor

   Depositor

   Nicolas Demaurex

   Article

   Poburko et al J Biol Chem. 2011 Apr 1;286(13):11672-84

   Insert

   SypHer
   

   Expression

   Mammalian

   Mutation

   C199S

   Promoter

   CMV

   Available Since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pInducer20-Blast

   Plasmid

   #109334

   Purpose

   pInducer20 with added blasticidin resistance

   Depositor

   Jean Cook

   Article

   Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

   Type

   Empty backbone

   Use

   Lentiviral; Inducible expression

   Expression

   Mammalian

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST_FLAG-HUWE1

   Plasmid

   #187120

   Purpose

   mammalian cell expression of FLAG-HUWE1

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Tags

   1xFLAG

   Expression

   Mammalian

   Available Since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiCRISPR_DKO

   Plasmid

   #183192

   Purpose

   Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

   Depositor

   Lang Li

   Article

   Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6, mU6

   Available Since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP Cited2

   Plasmid

   #112246

   Purpose

   expression of EGFP-Cited2 in mammalian cells

   Depositor

   Simone Di Giovanni

   Insert

   Cited2 (CITED2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEMS-II-dCas13-APO1-YTH

   Plasmid

   #204767

   Purpose

   GEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)

   Depositor

   Kate Meyer

   Article

   Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   dCas13-NLS-APO1-YTH-HA
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   Ef1alpha

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pORANGE LOX Cloning template vector

   Plasmid

   #139651

   Purpose

   Cloning template for ORANGE-CAKE conditional knock-in constructs

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-FLAG-C7orf59

    Plasmid

    #42332

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

    Insert

    C7orf59 (LAMTOR4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV, SP6

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA HA HP1 gamma

    Plasmid

    #24080

    Depositor

    Naoko Tanese

    Article

    Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

    Insert

    HP1 gamma (CBX3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBTK622

    Plasmid

    #180592

    Purpose

    tdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1

    Depositor

    Jeffrey Barrick

    Article

    Suarez et al Nucleic Acids Res. 2020 May 7;48(8):4585

    Inserts

    tdk
    kanR
    

    Use

    Synthetic Biology

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28b_H6-Smt3-AesC-GFP

    Plasmid

    #234486

    Purpose

    Superfolder GFP C-terminally fused to His-Smt3-tagged AesC split intein

    Depositor

    Henning Mootz

    Article

    Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

    Insert

    C-terminal Fragment of the Aes123 PolB1 Split Intein
    

    Tags

    Hexahistidine Tag, Superfolder Green Fluorescent …

    Expression

    Bacterial

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. eSrtA(2A-9) pET29b

    Plasmid

    #75145

    Purpose

    reprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificity

    Depositor

    David Liu

    Article

    Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

    Insert

    reprogrammed sortase A variant
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    S102C A104H E105D K138P K152I N160K K162H T164N K…

    Promoter

    T7

    Available Since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHiUGE smFP-V5 donor ORF2

    Plasmid

    #200382

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CAG-Electra2-P2A-EGFP

    Plasmid

    #184936

    Purpose

    Expresses blue fluorescent protein Electra2 and EGFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Electra2-P2A-EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-Stx17

    Plasmid

    #45911

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    Syntaxin 17 (STX17 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. FLT3-ITD-MSCV-IRES-GFP

    Plasmid

    #184779

    Purpose

    Expresses FLT3-ITD (internal tandem duplication) constitutively active mutant in mammalian cells

    Depositor

    Timothy Ley

    Insert

    FLT3 (FLT3 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Activating internal tandem duplication (ITD) adde…

    Promoter

    MSCV

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDARMO_HUWE1_3xFLAG

    Plasmid

    #187155

    Purpose

    mammalian cell expression of HUWE1-3xFLAG

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDisplay-GRAB_SST1.0

    Plasmid

    #208664

    Purpose

    Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based somatostatin (SST) sensor GRAB_SST1.0
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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