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G5-CD40

Plasmid

#121166

Purpose

pMP71 vector expressing GFP - P2A - G5 - myc - CD40

Depositor

Gabriel Victora

Publication

Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

Insert

G5-CD40

Use

Retroviral

Tags

GFP and myc

Expression

Mammalian

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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EF1a_mCherry_P2A_Hygro_Barcode

Plasmid

#120426

Purpose

Barcoded lentiviral vector to express mCherry in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

mCherry

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCCLc-MND-A0201-SABR-Backbone

Plasmid

#119050

Purpose

3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.

Depositor

David Baltimore

Publication

Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

MND

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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HR_G3BP1-V5-APEX2-GFP

Plasmid

#105284

Purpose

homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus

Depositor

Eugene Yeo

Publication

Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

Insert

G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 Human, soybean)

Use

Human homologous recombination donor

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLNCX2-mEGFP-TSG101

Plasmid

#116925

Purpose

expresses mEGFP-TSG101 in mammalian cells

Depositor

Sanford Simon

Publication

Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

Insert

TSG101 (TSG101 Human)

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHR-hSyn-EGFP

Plasmid

#114215

Purpose

Lentiviral vector (control) that targets expression of EGFP to neurons

Depositor

Xue Han

Publication

Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

Insert

Enhanced Green Fluorescent Protein

Use

Lentiviral and Mouse Targeting

Tags

None

Expression

Mammalian

Mutation

None

Promoter

human synapsin (hSyn)

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pMK231 (AAVS1 CMV-MCS-PURO)

Plasmid

#105924

Purpose

Donor for integration at the human AAVS1 locus

Depositor

Masato Kanemaki

Publication

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEJS614_pTetR-P2A-BFPnls/sgNS

Plasmid

#108650

Purpose

Non-specific Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Publication

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Non-specific sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLentiPGK Hygro DEST H2B-mRuby2

Plasmid

#90236

Purpose

Lentiviral vector to express H2B-mRuby2 under PGK promoter (With Hygromycin Resistance)

Depositor

Markus Covert

Publication

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

H2B

Use

Lentiviral

Tags

mRuby2

Expression

Mammalian

Promoter

PGK

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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SunTagng22aa

Plasmid

#106438

Purpose

Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.

Depositor

Steven Jacobsen

Publication

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CKIIa-stGtACR2-FusionRed

Plasmid

#105669

Purpose

Expresses a soma-targeted GtACR2 in mammalian neurons under the CaMKIIa promoter

Depositor

Ofer Yizhar

Publication

Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

Has service

AAV1 and AAV Retrograde

Insert

stGtACR2

Use

AAV

Tags

FusionRed and Kv2.1

Expression

Mammalian

Promoter

CaMKIIa

Available since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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MBP-hnRNPA2_FL_WT

Plasmid

#98662

Purpose

Expresses MBP-tagged full length hnRNPA2

Depositor

Nicolas Fawzi

Publication

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

6xHis-MBP

Expression

Bacterial

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pXPR_206

Plasmid

#96920

Purpose

for SaCas9 knockout, lentiviral expression of S. aureus Cas9 (SauCas9) and gRNA scaffold

Depositor

John Doench, David Root

Publication

Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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EGFP-Rab8a-wt

Plasmid

#86075

Purpose

A mammalian expression plasmid encoding Rab8a wild type with N-terminus EGFP.

Depositor

Lei Lu

Publication

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hRab8A (RAB8A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCMV-DeAct-GS1

Plasmid

#89445

Purpose

Expresses EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells

Depositor

Brad Zuchero

Publication

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DeAct-GS1 (GSN Human)

Tags

EGFP

Expression

Mammalian

Mutation

Truncation spanning cytoplasmic Gsn amino acids 5…

Promoter

CMV

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pT7-NSP3SA11

Plasmid

#89170

Purpose

Express Rotavirus SA11 NSP3 in mammalian cells

Depositor

Takeshi Kobayashi

Publication

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rotavirus SA11 NSP3

Expression

Mammalian

Promoter

T7 promotor

Available since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET-BiFC

Plasmid

#87856

Purpose

pETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.

Depositor

Dan Mulvihill

Publication

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Inserts

Encodes for MCS & C-term half of mVenus
Encodes for MCS & N-term half of mVenus

Tags

BiFC C-terminal fragment

Expression

Bacterial

Promoter

T7

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDNA3_UnaG-Flag-Sec61B

Plasmid

#83413

Purpose

Expressed N-term tagged UnaG on Sec61B

Depositor

Hyun-Woo Rhee

Publication

Park et al ACS Chem Biol. 2016 May 27.

Insert

UnaG-Sec61B (SEC61B Synthetic, Human)

Expression

Mammalian

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBK-VRER-BE3

Plasmid

#85173

Purpose

Expresses VRER-BE3 in mammalian cells

Depositor

David Liu

Publication

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VRER-BE3

Expression

Mammalian

Promoter

CMV

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pBK-YE1-BE3

Plasmid

#85174

Purpose

Expresses YE1-BE3 in mammalian cells

Depositor

David Liu

Publication

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

YE1-BE3

Expression

Mammalian

Promoter

CMV

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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