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Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling forces as a multi-arm ensemble.
Okumura M, Natsume T, Kanemaki MT, Kiyomitsu T
Elife. 2018 May 31;7. pii: 36559. doi: 10.7554/eLife.36559.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
105924pMK231 (AAVS1 CMV-MCS-PURO)Donor for integration at the human AAVS1 locus
105927hROSA26 CRISPR-pX330hROSA26 targeting CRISPR plasmid
114686pTK511Express Mem-BFP-iLID
114687pTK372sgRNA targeting NuMA's C-terminal locus
114688pTK421RFP-Nano tagging at NuMA's C-terminal locus
114689pTK308sgRNA targeting DHC's C-terminal locus
114690pTK565SNAP-tagging at DHC's C-terminal locus
114691pTK525sgRNA for p150's C-terminal locus
114692pTK587SNAP-tagging at p150's C-terminal locus
114693pTK371sgRNA for DHC's N-terminal locus
114694pTK426Nano-mCherry tagging at DHC's N-terminal locus
114695pTK583SNAP-tagging at NuMA's C-terminal locus
114696pTK600SNAP-tagging at DHC's C-terminal locus (BSD)
114697pTK630Express RFP-Nano from Rosa 26 locus
114698pTK715Express NuMA(1-2115)-RFP-Nano from Rosa 26 locus
114699pTK713Express NuMA(1-2115ΔNLS)-RFP-Nano from Rosa 26 locus
114700pTK652Express NuMA(1-1700)-RFP-Nano from Rosa 26 locus
114701pTK631Express NuMA(1-705)-RFP-Nano from Rosa 26 locus
114702pTK660Express NuMA(1-705)4A-RFP-Nano from Rosa 26 locus
114703pTK643Express NuMA(214-705)-RFP-Nano from Rosa 26 locus
114704pTK664Express NuMA(1-505)-RFP-Nano from Rosa 26 locus
114705pTK641Express NuMA(1-413)-RFP-Nano from Rosa 26 locus
114706pTK642Express NuMA(1-213)-RFP-Nano from Rosa 26 locus
114707pTK678Express NuMA(1-1895)-RFP-Nano from Rosa 26 locus
114708pTK711Express NuMA(1-1985)-RFP-Nano from Rosa 26 locus
114710pTK729Express NuMA(1-705+1700-2115ΔNLS)-RFP-Nano from Rosa 26 locus
114711pTK645Express NuMA(1700-2115)-RFP-Nano from Rosa 26 locus
114712pTK746Express NuMA(1-2115ΔNLS)5A-3-RFP-Nano from Rosa 26 locus
114713pTK388mAID-mClover-3FLAG tagging at DHC's C-terminal locus
114714pTK398mAID-mClover-3FLAG tagging at NuMA's C-terminal locus
114715pTK473sgRNA for LGN's N-terminal locus
114716pTK586SNAP-tagging at LGN's N-terminal locus (BSD)
114717pTK503Express mCherry-NuMA(WT) from Rosa 26 locus
114718pTK750Express mCherry-NuMA(5A-3) from Rosa 26 locus

Antibodies from Article