C-BERST: defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2.
Gao XD, Tu LC, Mir A, Rodriguez T, Ding Y, Leszyk J, Dekker J, Shaffer SA, Zhu LJ, Wolfe SA, Sontheimer EJ
Nat Methods. 2018 Jun;15(6):433-436. doi: 10.1038/s41592-018-0006-2. Epub 2018 May 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 108570 | pEJS578_DD-dSpyCas9-mCherry-APEX2 | Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors |
| 108649 | pEJS613_pTetR-P2A-BFPnls/sgTelo | Telomere-targeting Spy sgRNA under U6 promoter |
| 108650 | pEJS614_pTetR-P2A-BFPnls/sgNS | Non-specific Spy sgRNA under U6 promoter |
| 108651 | pEJS887_pTetR-P2A-BFPnls/sgAlpha | Alpha satellite repeats targeting Spy sgRNA under U6 promoter |