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(Plasmid #108651)


Item Catalog # Description Quantity Price (USD)
Plasmid 108651 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 8000
  • Total vector size (bp) 8610
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Alpha satellite repeats targeting sgRNA
  • Species
  • Insert Size (bp)
  • Promoter U6
  • Tag / Fusion Protein
    • No

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • Promoter hPGK

Cloning Information for Gene/Insert 2

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pTetR-P2A-BFPnls-sgRNA1-2XBroccoli-C3-6mer was a gift from David Grunwald Lab (published in Ma et al J. Cell Biol. 2016).

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

CRISPR-Cas9 nuclear dynamics and target recognition in living cells. Ma, H., Tu, L.-C., Naseri, A., Huisman, M., Zhang, S., Grunwald, D., and Pederson, T. (2016). CRISPR-Cas9 nuclear dynamics and target recognition in living cells. The Journal of Cell Biology 214, 529–537.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEJS887_pTetR-P2A-BFPnls/sgAlpha was a gift from Erik Sontheimer (Addgene plasmid # 108651 ; ; RRID:Addgene_108651)
  • For your References section:

    C-BERST: defining subnuclear proteomic landscapes at genomic elements with dCas9–APEX2. Gao , Xin D., Li-Chun Tu, Aamir Mir, Tomás Rodriguez, Yuehe Ding, John Leszyk, Job Dekker, Scott A. Shaffer, Lihua Julie Zhu, Scot A. Wolfe & Erik J. Sontheimer. Nature Methods (2018) 10.1038/s41592-018-0006-2