162,536 results

1. pME Actin Vhh mCherry (JDW 1240)

   Plasmid

   #224499

   Purpose

   Gateway compatible middle entry clone containing an Actin nanobody fused to mCherry (For visualizing actin, actin chromobody)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-mCherry
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. 24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)

   Plasmid

   #128604

   Purpose

   Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Inserts

   24xMoonTag
   kinesin family member 18b (KIF18B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Pcmv

   Available since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV6-AC EJ7-GFP

   Plasmid

   #113617

   Purpose

   Reporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFP

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   EJ7-GFP variant of EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-tTRKRAB-red

   Plasmid

   #12250

   Depositor

   Didier Trono

   Article

   Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

   Insert

   TetR-KRAB fusion and dsRED
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   dsRed added to backbone.

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.CMV.LacZ.bGH

   Plasmid

   #105531

   Purpose

   AAV expression of LacZ from CMV promoter

   Depositor

   James M. Wilson

   Has Service

   AAV5 and AAV8

   Insert

   LacZ
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. dCas13d-dsRBD-APEX2

   Plasmid

   #154939

   Purpose

   TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP

   Depositor

   Alice Ting

   Article

   Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

   Insert

   TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-P3-Alb-mScarlet

   Plasmid

   #183461

   Purpose

   To produce AAV with the P3 promoter to express Alb(mouse)-mScarlet fusion protein

   Depositor

   Hajime Hirase

   Article

   Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

   Insert

   Alb (Alb Mouse)
   

   Use

   AAV

   Tags

   mScarlet

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpliceExpress

   Plasmid

   #32485

   Depositor

   Stefan Stamm

   Article

   Kishore et al Gene. 2008 Dec 31;427(1-2):104-10. Epub

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pK170.AAV-TRE-Cre-WPRE (Supernova)

   Plasmid

   #85040

   Purpose

   For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   nlsCre-WPRE
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEJS1096 Dual-sgRNA.Design 1

    Plasmid

    #159538

    Purpose

    Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

    Depositor

    Erik Sontheimer

    Article

    Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

    Insert

    Nme2Cas9 nuclease with two guide RNA cassettes with promoters
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-ShadowG

    Plasmid

    #104620

    Purpose

    Expresses Shadow G in mammalian cell

    Depositor

    Hideji Murakoshi

    Article

    Murakoshi et al Sci Rep. 2015 Oct 15;5:15334. doi: 10.10

    Insert

    ShadowG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 5, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. GFP-UBR5

    Plasmid

    #52050

    Purpose

    CMV driven mammalian expression of UBR5 with an N-termianl GFP fusion

    Depositor

    Darren Saunders

    Article

    Gudjonsson et al Cell. 2012 Aug 17;150(4):697-709. doi: 1

    Insert

    UBR5 (UBR5 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-anti-GFP(LaG2) PAGER(TF)

    Plasmid

    #229996

    Purpose

    Expresses anti-GFP PAGER(TF) (high sensitivity) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    IL2SP-Aro6-LaG2-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    V360A/R361A on KORD

    Promoter

    CMV

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTlpA-Wasabi

    Plasmid

    #85455

    Purpose

    mWasabi reporter gene gated by wild type TlpA

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TlpA
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D341A

    Promoter

    TlpA and TlpA, LacI

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2

    Plasmid

    #167296

    Purpose

    Plasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFP

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    BAX (BAX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLNCX2 ER:ras

    Plasmid

    #67844

    Purpose

    4-hydroxytamoxifen inducible ER:ras fusion protein

    Depositor

    Masashi Narita

    Article

    Young et al Genes Dev. 2009 Apr 1;23(7):798-803. doi

    Insert

    ER1a H-RasG12V fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G525R renders the HBD largely insensitive to 17ß‐…

    Promoter

    CMV

    Available since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. HA-Rab7A

    Plasmid

    #131417

    Purpose

    Expresses HA-tagged human Rab7A in mammalian cells

    Depositor

    FENG-QIAN Li, Ken-Ichi Takemaru

    Insert

    human Rab7A
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-myc-Atg7

    Plasmid

    #24921

    Depositor

    Toren Finkel

    Article

    Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

    Insert

    Atg7 (ATG7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lyn-FKBP-FKBP-CFP (LF2C)

    Plasmid

    #20149

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    Lyn-FKBP-FKBP-CFP (FKBP1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See depositor comments below.

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. EBFP2-N1

    Plasmid

    #54595

    Purpose

    Localization: N1 Cloning Vector, Excitation: 383, Emission: 448

    Depositor

    Michael Davidson

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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