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pHR-Traditional CAR (anti-CD19)

Plasmid

#192115

Purpose

Lentiviral delivery of Traditional CAR(anti-CD19)

Depositor

Wilson Wong

Article

Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

Insert

pHR-pEF1a-Traditional CAR (anti-CD19scFV_CD28_41BB_CD3z)

Use

Lentiviral

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-MSCV-hCD38-EF1α-GFP+Puro

Plasmid

#134936

Purpose

expression human CD38 in mammalian cells

Depositor

Jianjun Zhao

Article

Hu et al Cancer Res. 2020 Mar 19. pii: 0008-5472.

Insert

CD38 (CD38 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLX307-VHL(Y98H)-3F-BirA

Plasmid

#220148

Purpose

Expresses VHL(Y98H)-BirA as a control for VHL substrate identification by E-STUB

Depositor

William Sellers

Article

Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

Insert

von Hippel-Lindau tumor suppressor (VHL Human)

Use

Lentiviral

Tags

3F-BirA

Expression

Mammalian

Mutation

changed tyrosine 98 to histidine; codon optimized

Promoter

EF-1a

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC11

Plasmid

#231659

Purpose

Expresses human ZDHHC11 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC11 (ZDHHC11 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti PGK Puro DEST (w529-2)

Plasmid

#19068

Purpose

3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-cmyc

Plasmid

#16011

Purpose

mammalian expression of human cmyc

Depositor

Wafik El-Deiry

Article

Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

Insert

cmyc (MYC Human)

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pAG261-His-greenFAST

Plasmid

#160349

Purpose

Expresses His-tagged greenFAST in bacteria

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

His-greenFAST

Expression

Bacterial

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP_mNG2(11)_Clathrin light chain

Plasmid

#82608

Purpose

Expresses mNG2(11) tagged clathrin light chain in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

mNG2(11)_Clathrin light chain

Expression

Mammalian

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-IRAlus-Lin28

Plasmid

#92347

Purpose

Plasmid for expression of GFP gene with a Lin28 gene derived IR Alu element containing 3'UTR.

Depositor

Gordon Carmichael, Ling-Ling Chen

Article

Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

Insert

Lin28 gene derived IR Alu element containing 3'UTR (LIN28A Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-dxCas9(3.7)-VPR-EF1a-TagRFP-2A-tet3G

Plasmid

#167937

Purpose

Inducible CRISPRa expression

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

dxCas9(3.7)-VPR

Use

Lentiviral

Promoter

tetON

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Rab9a

Plasmid

#169071

Purpose

Express human Rab9a in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rab9a (RAB9A Human)

Tags

acGFP1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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XRE-H2B-eGFP

Plasmid

#182294

Purpose

Fluorescent reporter for AhR activity

Depositor

Severine Degrelle, Thierry Fournier

Article

Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

Insert

Xenobiotic Response Element (CYP1A1 Human)

Tags

eGFP

Expression

Mammalian

Promoter

XRE

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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LCV2_EGFP

Plasmid

#155098

Purpose

Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and EGFP expression

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-EA-evoAPOBEC1max-32NLS_NG-BlastR

Plasmid

#232722

Purpose

C-to-T base editor

Depositor

David Liu

Article

Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

Insert

evoAPOBEC1max H47E+S48A-32NLS linker-nCas9(NG)-UGI-UGI

Expression

Mammalian

Mutation

within evoAPOBEC1: H47E+S48A; within Cas9 D10A

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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p2T-U6-sgCTG

Plasmid

#232723

Purpose

SpCas9 guide RNA targeting CAG repeats for C-to-T base editing

Depositor

David Liu

Article

Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

Insert

SpCas9 gRNA targeting CAG repeats

Expression

Mammalian

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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TRUPATH Triple Gai3

Plasmid

#196050

Purpose

Encodes a G alpha subunit (GNAl3) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

Depositor

Justin English

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Inserts

GAlphai3-RLuc8 (GNAI3 Human)
GGamma9-GFP2
GBeta3 (GNB3 Human)

Use

Luciferase

Tags

GFP2 and GSAG linker

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-1

Plasmid

#163127

Purpose

pLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Tags

Ubiquitin

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Pink Flamindo-p2a-bPAC

Plasmid

#202716

Purpose

Mammalian co-expression of soluble Pink Flamindo, a fluorescent cAMP indicator and soluble bPAC, a photoactivatable adenylyl cyclase

Depositor

Adam Cohen

Article

Xiang et al bioRxiv 2023

Inserts

Pink Flamindo
bPAC

Use

Lentiviral

Tags

10xHis and myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4

Plasmid

#192807

Purpose

Stable expression of 3xFLAG-NCOA4 in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pX330S-2-PITCh

Plasmid

#63670

Purpose

Expresses Cas9 nuclease and the PITCh-gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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