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pEF1α-Cre-2A-mTagBFP2

Plasmid

#207746

Purpose

Expression of the Cre recombinase linked to a mTagBFP2 fluorescent marker

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

Cre-PGK-mTagBFP2

Use

Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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AAV-KrasG13D/sgKras/Cre

Plasmid

#99857

Purpose

Expresses Cre-recombinase, a barcoded HDR template to introduce a G13D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

Depositor

Monte Winslow

Article

Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

Insert

Kras (Kras Mouse)

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_B2519

Plasmid

#91076

Purpose

Module B, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning

Use

CRISPR

Promoter

OsU3

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp15-mNeongreen

Plasmid

#182389

Purpose

Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp15-mNeongreen-His

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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VfAU1-LOV_136

Plasmid

#63632

Purpose

VfAU1-LOV domain (Vaucheria frigida Aureochrome 1) preceded by a restriction site

Depositor

Harald Janovjak

Insert

VfAU1-LOV

Expression

Mammalian

Available Since

April 7, 2015

Availability

Academic Institutions and Nonprofits only

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MTK0_002

Plasmid

#123960

Purpose

Encodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB Destination - 2xHS4 - HygroR

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-Fis1

Plasmid

#182580

Purpose

Expresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

Depositor

Uri Manor

Article

Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

Insert

mCherry-Fis1

Tags

Fis1

Expression

Mammalian

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-BHLHE40(S243A/E248A)

Plasmid

#110155

Purpose

Expression of human BHLHE40 (S243A/E248A) in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

Insert

BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)

Tags

HA

Expression

Mammalian

Mutation

S243A/E248A

Promoter

CMV

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pS548∙CsR

Plasmid

#197859

Purpose

Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

gRNA scaffold - Tc resistance cassette - incP oriT

Use

CRISPR and Synthetic Biology

Promoter

PEM7

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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lenti SYN-FLAG-dCas9-VPR

Plasmid

#114196

Purpose

Lentivirus compatible dCas9-VPR construct driven by the human SYN promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

dCas9-VPR

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

SYN

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pdCas9-sgRNA-RFP

Plasmid

#166005

Purpose

Bacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)

Depositor

Martin Buck

Article

Bradley et al Biotechnol Rep (Amst). 2021 Oct 9;32:e00

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

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LLP339 pGK-dCas9-SunTag

Plasmid

#100943

Purpose

dCas9 vector with SunTagx10, driven by pGK, with 3xHA tag, Puro driven by EF1a

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

dCas9, SunTag array (10xGCN4)

Use

CRISPR

Tags

3xHA

Expression

Mammalian

Mutation

D10A, H840A for dCas9

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP

Plasmid

#158962

Purpose

Cre-dependent Cas9-GFP expression

Depositor

Michael Brand

Article

Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

Inserts

hsp70l promoter
floxed DsRed cassette
Cas9-GFP

Use

CRISPR and Cre/Lox

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-FRT hM4Di-mCherry

Plasmid

#161576

Purpose

Expression of hM4Di-mCherry in a Flp-dependent fashion

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

hM4Di-mCherry

Use

AAV

Mutation

NA

Promoter

hSyn

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pQCXIN-turboRFP

Plasmid

#138275

Purpose

Retroviral expression of turboRFP

Depositor

Michael Grusch

Insert

TurboRFP

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLJM60-FLAG NUFIP1

Plasmid

#110950

Purpose

expresses mammalian FLAG-NUFIP1

Depositor

David Sabatini

Article

Wyant et al Science. 2018 May 18;360(6390):751-758.

Insert

NUFIP1 (NUFIP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a - NDH

Plasmid

#89626

Purpose

Expresses recombinant 6HIS tagged T. cruzi NADP-dependent oxidoreductase in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

NAD(P)-dependent oxidoreductase

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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PH-Akt-GFP

Plasmid

#51465

Purpose

Is a biosensor for PIP3/PI(3,4)P2

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

AKT (aa 1–164)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pB-EF1a-CuO-KRAB-dCas9-BFP-CymR-Puro

Plasmid

#248161

Purpose

Expresses KRAB fused to dCas9 and BFP upon cumate induction

Depositor

Joanna Wysocka

Article

Fueyo et al Nature. 2025 Oct 1. doi: 10.1038/s41586-

Inserts

KRAB-dCas9-HA-tagBFP
HA-CymR-T2A-puro

Use

CRISPR

Tags

HA and tagBFP

Expression

Mammalian

Promoter

Ef1a

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE3G-XRI-FLAG

Plasmid

#250671

Purpose

Timestamp monomer for encoding time along the CytoTape-vivo fiber for mouse brain in vivo applications.

Depositor

Changyang Linghu

Article

Zheng et al Nature. 2026 Jan 26. doi: 10.1038/s41586

Insert

XRI-FLAG

Use

AAV

Expression

Mammalian

Promoter

TRE3G

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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