165,347 results

1. pCBC-MT1T2

   Plasmid

   #50593

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Insert

   T1, gRNA scaffold, OsU3t plus, TaU3p, T2
   

   Use

   CRISPR; Pcr template

   Expression

   Plant

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAM-AAV-hSyn1-EGFP

   Plasmid

   #153205

   Purpose

   Human synapsin-1 (hSyn1) promoter-mediates gene expression in retinal neurons with fluorescent reporter

   Depositor

   Yang Hu

   Article

   Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Available Since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV p16 INK4A

   Plasmid

   #10916

   Depositor

   Bob Weinberg

   Article

   Medema et al Proc Natl Acad Sci U S A. 1995 Jul 3. 92

   Insert

   p16 INK4 (CDKN2A Human)
   

   Expression

   Mammalian

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCX460

   Plasmid

   #229215

   Purpose

   CRISPR array plasmid for MAP1B (Spacers 25-48)

   Depositor

   Jennifer Doudna

   Article

   Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

   Insert

   CRISPR array plasmid for MAP1B (Spacers 25-48)
   

   Expression

   Mammalian

   Available Since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAV-hSynI-rtTAV16

   Plasmid

   #102367

   Purpose

   AAV vector to drive TET-ON activator expression from human synapsin I promoter

   Depositor

   Tetsuo Yamamori

   Article

   Watakabe et al Front Neural Circuits. 2014 Sep 17;8:110

   Insert

   rtTAV16
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   rtTA variant reported in Gene Therapy (2006) 13, …

   Promoter

   human synapsin I promoter

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGST-PSP-hTG

   Plasmid

   #85470

   Purpose

   bacterial expression of human tissue transglutaminase

   Depositor

   Ray Truant, Jeffrey Keillor

   Article

   Roy et al Protein Expr Purif. 2013 Jan;87(1):41-6.

   Insert

   hTG2 (TGM2 Human)
   

   Tags

   GST and PSP

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Rabbit GFP Cav 2.2 pMT2

   Plasmid

   #58737

   Purpose

   expression of GFP tagged rabbit Cav2.2 in mammalian cells

   Depositor

   Annette Dolphin

   Article

   Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

   Insert

   Cav2.2 (CACNA1B Rabbit)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET30b-7+-SrtA

   Plasmid

   #105602

   Purpose

   expressed 7+ sortase A variant in E.coli

   Depositor

   Stephanie Dougan

   Article

   Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

   Insert

   S.aureus Sortase A, 7+ mutant
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAG1160 mCherry-FIREtag

   Plasmid

   #205164

   Purpose

   Expresses mCherry -FIREtag in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   mCherry-FIREtag
   

   Expression

   Mammalian

   Available Since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRSET-MagLOV

    Plasmid

    #219957

    Purpose

    Bacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2

    Depositor

    Andrew York

    Article

    Frank Hayward et al (2023) doi: 10.5281/zenodo.8137174

    Insert

    MagLOV
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    AsLOV2 C450P L496V Q513K G528K D540M

    Promoter

    T7

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Lenti-LucOS

    Plasmid

    #22777

    Depositor

    Tyler Jacks

    Insert

    OVA-SIY
    

    Use

    Cre/Lox and Lentiviral

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAF0451 Si74_attP-mCherry donor

    Plasmid

    #193470

    Purpose

    Promoterless mcherry donor for Si74

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Si74_attP
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TFORF1917

    Plasmid

    #143554

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RUNX2 (RUNX2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYLCRISPR/Cas9P35S-B

    Plasmid

    #66190

    Purpose

    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    cauliflower mosaic virus 35S promoter (P35S)

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SIRT5 Flag

    Plasmid

    #13816

    Purpose

    Mammalian expression of human SIRT5 with flag tag

    Depositor

    Eric Verdin

    Article

    North et al Mol Cell. 2003 Feb . 11(2):437-44.

    Insert

    SIRT5 (SIRT5 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVX-EF1a-GFP-LaminA-IRES-Hygromycin

    Plasmid

    #134867

    Purpose

    Expresses EGFP-tagged nuclear envelope marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Lamin A (LMNA Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP-C3-Exo70

    Plasmid

    #53761

    Purpose

    pEGFP-C3 plasmid that expresses Exo70

    Depositor

    Channing Der

    Article

    Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

    Insert

    Exo70 (EXOC7 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Flag-FOXP3

    Plasmid

    #153147

    Purpose

    Expression of Flag tagged human FOXP3 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXP3 (FOXP3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. sFlt1-e15a V5

    Plasmid

    #86045

    Purpose

    human Soluble Flt1 isoform e15a (terminates in exon 15a)

    Depositor

    Christie Thomas

    Article

    Thomas et al J Clin Endocrinol Metab. 2009 Jul;94(7):

    Insert

    human soluble Flt1 isoform e15a (FLT1 Human)
    

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    isoform e15a (terminates in exon 15a)

    Promoter

    CMV

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLv-HSA-uDys/eGFP

    Plasmid

    #26810

    Depositor

    Jeffrey Chamberlain

    Article

    Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

    Insert

    dystrophin (DMD Human)
    

    Use

    Lentiviral; Lentiviral plasmid backbone from l. n…

    Tags

    eGFP

    Mutation

    deleted R4-R23 deleted exons 71-78

    Available Since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge