|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13332||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameDNA ligase IV
SpeciesH. sapiens (human)
Entrez GeneLIG4 (a.k.a. LIG4S)
/ Fusion Protein
- His (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Full length DNA ligase IV was fused to a polyhistidine tag at the C-terminus and subcloned into pET28a.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDR119 was a gift from Martin Gellert (Addgene plasmid # 13332 ; http://n2t.net/addgene:13332 ; RRID:Addgene_13332)
For your References section:DNA binding of Xrcc4 protein is associated with V(D)J recombination but not with stimulation of DNA ligase IV activity. Modesti M, Hesse JE, Gellert M. EMBO J. 1999 Apr 1. 18(7):2008-18. 10.1093/emboj/18.7.2008 PubMed 10202163