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pDR119
(Plasmid #13332)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13332 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DNA ligase IV
  • Species
    H. sapiens (human)
  • Entrez Gene
    LIG4 (a.k.a. LIG4S)
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

Full length DNA ligase IV was fused to a polyhistidine tag at the C-terminus and subcloned into pET28a.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDR119 was a gift from Martin Gellert (Addgene plasmid # 13332 ; http://n2t.net/addgene:13332 ; RRID:Addgene_13332)
  • For your References section:

    DNA binding of Xrcc4 protein is associated with V(D)J recombination but not with stimulation of DNA ligase IV activity. Modesti M, Hesse JE, Gellert M. EMBO J. 1999 Apr 1. 18(7):2008-18. 10.1093/emboj/18.7.2008 PubMed 10202163