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Addgene
Modular design of synthetic receptors for programmed gene regulation in cell therapies.
Zhu I, Liu R, Garcia JM, Hyrenius-Wittsten A, Piraner DI, Alavi J, Israni DV, Liu B, Khalil AS, Roybal KT
Cell. 2022 Apr 14;185(8):1431-1443.e16. doi: 10.1016/j.cell.2022.03.023. PubMed Article

Plasmids from Article

ID Plasmid Purpose
188336pHR_PGK_SNIPR_hsNotch1 LNRLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
188338pHR_PGK_SNIPR_hsNotch3 LNRLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch3-ECD, a Notch3-TMD, a Notch3-JMD, and a Gal4VP64 transcriptional factor
188340pHR_PGK_SNIPR_synRoboLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Robo1-ECD, a Robo1-TMD, a Robo1-JMD, and a Gal4VP64 transcriptional factor
188341pHR_PGK_SNIPR_synRobo/NotchLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Robo1-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
188342pHR_PGK_SNIPR_FnIII-NotchLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a FnIII-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
188343pHR_PGK_SNIPR_(GGS)3_SNIPRLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)3, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
188344pHR_PGK_SNIPR_(GGS)6_SNIPRLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)6, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
188345pHR_PGK_SNIPR_(GGS)9_SNIPRLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)9, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
188346pHR_PGK_SNIPR_(GGS)12_SNIPRLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)12, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
188347pHR_PGK_SNIPR_(GGS)15_SNIPRLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)15, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
188348pHR_PGK_SNIPR_(GGS)18_SNIPRLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)18, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
188349pHR_PGK_SNIPR_ADAM17 siteLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a ADAM17site, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
188350pHR_PGK_SNIPR_FAP siteLentiviral vector - constitutive expression of an myctag SNIPR with a FAPsite, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
188351pHR_PGK_SNIPR_FAP site with scFvLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a FAPsite, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
188352pHR_PGK_SNIPR_3xFLAG-tag tetherLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a 3X-FLAG-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
188353pHR_PGK_SNIPR_Notch1 del-NRRLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-del-NRR-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
188354pHR_PGK_SNIPR_Notch2 del-NRRLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch2-del-NRR-ECD, a Notch2-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor
188355pHR_PGK_SNIPR_Notch3 del-NRRLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch3-del-NRR-ECD, a Notch3-TMD, a Notch3-JMD, and a Gal4VP64 transcriptional factor
188357pHR_PGK_SNIPR_CD8alpha SNIPRLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
188358pHR_PGK_SNIPR_CD28 SNIPRLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD28-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
188359pHR_PGK_SNIPR_IgG4 SNIPRLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a IgG4-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
188360pHR_PGK_SNIPR_OX40 SNIPRLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a OX40-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
188362pHR_PGK_SNIPR_CD8alpha variant 2Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
188363pHR_PGK_SNIPR_CD8alpha variant 3Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant3-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
188364pHR_PGK_SNIPR_CD8alpha variant 4Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant4-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
188365pHR_PGK_SNIPR_CD8alpha variant 2, G318ALentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD_G318A, a Notch1-JMD, and a Gal4VP64 transcriptional factor
188366pHR_PGK_SNIPR_CD8alpha variant 2, V319ALentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD_V319A, a Notch1-JMD, and a Gal4VP64 transcriptional factor
188374pHR_PGK_SNIPR_hsNotch1 LNR, Notch2 JMDLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor
188375pHR_PGK_SNIPR_Hinge Notch SNIPR (Notch2 JMD)Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor
188376pHR_PGK_ALPPL2 synNotchLentiviral vector - constitutive expression of an anti-ALPPL2 synNotch with a Gal4VP64 transcriptional factor
188377pHR_PGK_SNIPR_ALPPL2 Hinge Notch SNIPRLentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
188379pHR_PGK_SNIPR_Hinge Notch SNIPR (NRG1 JMD)Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a NRG1-JMD, and a Gal4VP64 transcriptional factor
188380pHR_PGK_SNIPR_Hinge Notch SNIPR (LaG17)Lentiviral vector - constitutive expression of an anti-GFP SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor
188383pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (Pax6)Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Pax6(DBD)-p65(428-551) transcriptional factor
188384pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (HNF1A)Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor
188385pHR_PGK_SNIPR_Hinge Notch SNIPR (HNF1A, ALPPL2 scFv)Lentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor
188386pHR_Gal4UAS_tBFP_PGK_mCitrineLentiviral vector - tBFP reporter for Gal4-based SNIPRs with a constitutive mCitrine;
188387pHR_HNF1A-RE_tBFP_PGK_mCitrineLentiviral vector - tBFP reporter for HNF1A-based SNIPRs with a constitutive mCitrine;
188388pHR_Pax6-RE_tBFP_PGK_mCitrineLentiviral vector - tBFP reporter for Pax6-based SNIPRs with a constitutive mCitrine

Antibodies from Article