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169,217 results

Showing: 6981 - 7000 of 169217 results

  3. pDD162 (Peft-3::Cas9 + Empty sgRNA)

    Plasmid
    #47549
    Purpose
    Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm
    Inserts
    Cas9
    Empty sgRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Worm
    Mutation
    Codon optimized and with synthetic introns for C.…
    Promoter
    U6 and eef-1A.1 (eft-3)
    Available Since
    Sept. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    47549-plasmid-map-sequence-id-228099

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  5. pJB158

    Plasmid
    #248751
    Purpose
    PopuA+20xTetO biosensor cicuit in pNBU2 integrative Bacteroides plasmid
    Depositor
    Carolina Tropini
    Insert
    PBfP1E6-RBS1PLP-mScarletI3-rrnBT2-20xTetO-T7Te-rrnBT1-PopuA-TetR-rrnBT2-pTetR56-RBS1PLP-mGL-rrnBT2
    Expression
    Bacterial
    Available Since
    Jan. 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    248751-plasmid-map-sequence-id-496751

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  6. pEM351

    Plasmid
    #248763
    Purpose
    80xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate Assembly
    Depositor
    Carolina Tropini
    Insert
    T7Te-rrnBT1-80xTetO
    Expression
    Bacterial
    Available Since
    Jan. 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    248763-plasmid-map-sequence-id-496748

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  10. pEM414

    Plasmid
    #248766
    Purpose
    20xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate Assembly
    Depositor
    Carolina Tropini
    Insert
    T7Te-rrnBT1-20xTetO
    Expression
    Bacterial
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248766-plasmid-map-sequence-id-496747

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  11. tetO-HyperTRIBE-adar_deaminase-V5-t2a-mCherry

    Plasmid
    #241314
    Purpose
    inducible mouse Adar Deaminase domain (HyperTRIBE) for expression in mammalian cells
    Depositor
    Stavros Lomvardas
    Insert
    Adar deaminase domain (Adar Mouse)
    Tags
    V5
    Expression
    Mammalian
    Mutation
    E957Q
    Promoter
    tetO and CMV
    Available Since
    Oct. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    241314-plasmid-map-sequence-id-478721

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  16. TFORF1113

    Plasmid
    #143928
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RCOR3 (RCOR3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    143928-plasmid-map-sequence-id-471142

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  17. EV-A71 isolate 4643-TW98 2A precursor protease with C110A and four aa at N-term to create a VP1-2A junction in active site

    Plasmid
    #228633
    Purpose
    Bacterial expression of the A71 2A protease with ITTL at the N-terminal and a C110A mutation used for crystallization in P 6 1 (PDB 9FGO)
    Depositor
    ASAP Discovery, Frank von Delft
    Insert
    Enterovirus Coxsackievirus A71 2A protease
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    C110A
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228633-plasmid-map-sequence-id-464103

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  18. PPE18-ALFA

    Plasmid
    #231247
    Purpose
    Expresses a C-terminally tagged PPE18 (ALFA tag)
    Depositor
    Bryan Bryson
    Insert
    PPE18-ALFA (PPE18 Mycobacterium tuberculosis)
    Tags
    ALFA Tag
    Expression
    Bacterial
    Promoter
    hsp60
    Available Since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231247-plasmid-map-sequence-id-463614

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  20. pET28-mEGFP(A206K)-TwStrep

    Plasmid
    #208622
    Purpose
    for E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) clone
    Depositor
    Quanfu Ma
    Insert
    mEGFP(A206K)
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    (compared with pEGFP-C1, protein level) A206K
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208622-plasmid-map-sequence-id-415916

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Showing: 6981 - 7000 of 169217 results