Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi).
Cleto S, Jensen JV, Wendisch VF, Lu TK
ACS Synth Biol. 2016 Feb 16. PubMed Article
Cleto S, Jensen JV, Wendisch VF, Lu TK
ACS Synth Biol. 2016 Feb 16. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 74062 | pZ8-T_dCas9 | pZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanR |
| 74063 | pZ8-P_dCas9 | pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanR |
| 74064 | pZ8-Ptac | IPTG inducible version of pZ8-1 plasmid, to which lacIq was added, KanR |
| 74065 | pZ8-Prp | pZ8-1 with the PprpD2 propionate-inducible promoter instead of Ptac, KanR |
| 74066 | pAL-pgi_T | pAL374 plasmid carrying the pgi (T) sgRNA, targeting the template strand of pgi, SpecR |
| 74067 | pAL-pgi_NT | pAL374 plasmid carrying the pgi (NT) sgRNA, targeting the non-template strand of pgi, SpecR |
| 74068 | pAL-pck_T | pAL374 plasmid carrying the pck (T) sgRNA targeting, the template strand of pck, SpecR |
| 74069 | pAL-pck_NT | pAL374 plasmid carrying the pck (NT) sgRNA, targeting the non-template strand of pck, SpecR |
| 74070 | pAL-pyk_T | pAL374 plasmid carrying the pyk (T) sgRNA targeting the template strand of pyk, SpecR |
| 74072 | pAL-pyk_NT | pAL374 plasmid carrying the pyk (NT) sgRNA targeting the non-template strand of pyk, SpecR |
| 74073 | pRIM3-rfp | GentR version of pRIM2 carrying rfp |
| 74075 | pAL-rfp_T | pAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecR |
| 79642 | Corynebacterium glutamicum::RFP | Expresses RFP from the chromosome of Corynebacterium glutamicum ATCC 13032 (ptac promoter unrepressed; no LacI present) |