Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi).
Cleto S, Jensen JV, Wendisch VF, Lu TK
ACS Synth Biol. 2016 Feb 16.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 74062 | pZ8-T_dCas9 | pZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanR |
| 74063 | pZ8-P_dCas9 | pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanR |
| 74064 | pZ8-Ptac | IPTG inducible version of pZ8-1 plasmid, to which lacIq was added, KanR |
| 74065 | pZ8-Prp | pZ8-1 with the PprpD2 propionate-inducible promoter instead of Ptac, KanR |
| 74066 | pAL-pgi_T | pAL374 plasmid carrying the pgi (T) sgRNA, targeting the template strand of pgi, SpecR |
| 74067 | pAL-pgi_NT | pAL374 plasmid carrying the pgi (NT) sgRNA, targeting the non-template strand of pgi, SpecR |
| 74068 | pAL-pck_T | pAL374 plasmid carrying the pck (T) sgRNA targeting, the template strand of pck, SpecR |
| 74069 | pAL-pck_NT | pAL374 plasmid carrying the pck (NT) sgRNA, targeting the non-template strand of pck, SpecR |
| 74070 | pAL-pyk_T | pAL374 plasmid carrying the pyk (T) sgRNA targeting the template strand of pyk, SpecR |
| 74072 | pAL-pyk_NT | pAL374 plasmid carrying the pyk (NT) sgRNA targeting the non-template strand of pyk, SpecR |
| 74073 | pRIM3-rfp | GentR version of pRIM2 carrying rfp |
| 74075 | pAL-rfp_T | pAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecR |
| 79642 | Corynebacterium glutamicum::RFP | Expresses RFP from the chromosome of Corynebacterium glutamicum ATCC 13032 (ptac promoter unrepressed; no LacI present) |