Corynebacterium glutamicum::RFP
(Bacterial strain #79642)

• Purpose: Expresses RFP from the chromosome of Corynebacterium glutamicum ATCC 13032 (ptac promoter unrepressed; no LacI present)
• Depositing Lab: Timothy Lu
• Publication: Cleto et al ACS Synth Biol. 2016 Feb 16.

Backbone

• Vector backbone: NA

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): This is a strain
• Growth instructions: Slow growing; may require longer incubation/growth time
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: NA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

If LacI is added to the cell (e.g., in a plasmid), the expression of RFP will be somewhat repressed (unless if IPTG is added), as there is a lacO between the promoter and the gene's ATG. Attempts to remove this lacO were unsuccessful - we suspect the higher RFP expression might lead to cell death (in the cloning strain, E. coli DH5alpha).

Please note this strain has a higher %GC than E. coli, so PCRing (and sequencing) may be difficult.