|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||74062||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Modifications to backboneAdded LacIq repressor
Vector typeBacterial Expression, CRISPR, Synthetic Biology
Growth in Bacteria
Copy numberLow Copy
- Promoter ptac
- Cloning method Gibson Cloning
- 5′ sequencing primer CGACATCATAACGGTTCTGGC (Common Sequencing Primers)
Plasmid used to repress genes in Corynebacterium glutamicum as described in Cleto et al., 2016. Note that the species of dCas9 in this plasmid is S. pyogenes.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pZ8-T_dCas9 was a gift from Timothy Lu (Addgene plasmid # 74062 ; http://n2t.net/addgene:74062 ; RRID:Addgene_74062)
For your References section:Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). Cleto S, Jensen JV, Wendisch VF, Lu TK. ACS Synth Biol. 2016 Feb 16. 10.1021/acssynbio.5b00216 PubMed 26829286