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AB-GFP
(Plasmid #72203)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 72203 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28(A+) based GFP folding reporter
  • Backbone manufacturer
    Waldo, et al. PMID 10404163
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    AB42
  • Alt name
    amyloid beta (A4) precursor protein
  • Alt name
    APP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    123
  • GenBank ID
    351
  • Entrez Gene
    APP (a.k.a. AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4)
  • Promoter T7
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer GFP-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AB-GFP was a gift from Michael Hecht (Addgene plasmid # 72203 ; http://n2t.net/addgene:72203 ; RRID:Addgene_72203)
  • For your References section:

    Mutations that reduce aggregation of the Alzheimer's Abeta42 peptide: an unbiased search for the sequence determinants of Abeta amyloidogenesis. Wurth C, Guimard NK, Hecht MH. J Mol Biol. 2002 Jun 21;319(5):1279-90. 10.1016/S0022-2836(02)00399-6 PubMed 12079364